Chlorine in PDB 4klm: Dna Polymerase Beta Matched Product Complex with MG2+, 11 H

All present enzymatic activity of Dna Polymerase Beta Matched Product Complex with MG2+, 11 H:
2.7.7.7;

The structure of Dna Polymerase Beta Matched Product Complex with MG2+, 11 H, PDB code: 4klm was solved by B.D.Freudenthal, W.A.Beard, D.D.Shock, S.H.Wilson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	23.83 / 1.75
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	50.588, 79.760, 55.338, 90.00, 107.70, 90.00
R / Rfree (%)	18.8 / 23.9

The structure of Dna Polymerase Beta Matched Product Complex with MG2+, 11 H also contains other interesting chemical elements:

Magnesium	(Mg)	1 atom
Sodium	(Na)	3 atoms

The binding sites of Chlorine atom in the Dna Polymerase Beta Matched Product Complex with MG2+, 11 H (pdb code 4klm). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Dna Polymerase Beta Matched Product Complex with MG2+, 11 H, PDB code: 4klm:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Dna Polymerase Beta Matched Product Complex with MG2+, 11 H


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Dna Polymerase Beta Matched Product Complex with MG2+, 11 H within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl406 b:30.0 occ:1.00 N A:HIS34 3.3 25.4 1.0 C A:ALA32 3.5 26.3 1.0 CB A:HIS34 3.5 32.2 1.0 CB A:ALA32 3.5 31.2 1.0 CA A:ALA32 3.6 33.8 1.0 N A:LYS35 3.6 28.9 1.0 N A:ILE33 3.8 26.1 1.0 CA A:HIS34 3.8 25.4 1.0 O A:ALA32 3.9 27.9 1.0 CD2 A:HIS34 3.9 42.6 1.0 CG A:HIS34 4.1 36.1 1.0 C A:HIS34 4.2 28.2 1.0 CG A:LYS35 4.2 26.6 1.0 C A:ILE33 4.3 29.6 1.0 O A:HOH576 4.5 38.0 1.0 CB A:LYS35 4.6 22.9 1.0 CA A:ILE33 4.6 33.2 1.0 CA A:LYS35 4.7 27.8 1.0 O6 D:DG1 4.8 30.6 1.0

Chlorine binding site 2 out of 2 in the Dna Polymerase Beta Matched Product Complex with MG2+, 11 H


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Dna Polymerase Beta Matched Product Complex with MG2+, 11 H within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl407 b:30.0 occ:1.00 NH2 A:ARG299 2.9 25.2 0.6 O A:HOH672 3.0 33.9 1.0 OG1 A:THR297 3.2 24.1 1.0 NE A:ARG299 3.3 24.9 0.6 ND2 A:ASN294 3.4 22.2 1.0 CZ A:ARG299 3.5 29.5 0.6 CG2 A:THR297 3.8 22.9 1.0 CB A:TYR296 4.0 21.7 1.0 OD1 A:ASN294 4.0 24.1 1.0 CZ A:ARG299 4.0 33.4 0.4 NH2 A:ARG299 4.1 27.2 0.4 CB A:THR297 4.1 25.2 1.0 NE A:ARG299 4.1 26.8 0.4 CG A:ASN294 4.1 22.6 1.0 CD2 A:TYR296 4.2 20.8 1.0 NH1 A:ARG299 4.5 25.3 0.4 CG A:TYR296 4.5 19.2 1.0 CD A:ARG299 4.6 27.6 0.6 NH1 A:ARG299 4.7 28.5 0.6 O A:HOH661 4.8 45.4 1.0 CD A:ARG299 4.8 27.8 0.4 N A:THR297 4.8 20.9 1.0 O A:HOH600 4.8 26.4 1.0 O T:HOH131 4.8 34.6 1.0

B.D.Freudenthal, W.A.Beard, D.D.Shock, S.H.Wilson. Observing A Dna Polymerase Choose Right From Wrong. Cell(Cambridge,Mass.) V. 154 157 2013.
ISSN: ISSN 0092-8674
PubMed: 23827680
DOI: 10.1016/J.CELL.2013.05.048