Chlorine in PDB 4lmj: Glic Liganded-Closed-Channel Conformation, Mutant T25'A

The structure of Glic Liganded-Closed-Channel Conformation, Mutant T25'A, PDB code: 4lmj was solved by C.Grosman, G.Gonzalez-Gutierrez, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.04 / 3.44
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	181.120, 134.320, 159.890, 90.00, 101.96, 90.00
R / Rfree (%)	22.7 / 25.5

The structure of Glic Liganded-Closed-Channel Conformation, Mutant T25'A also contains other interesting chemical elements:

Sodium

(Na)

5 atoms

The binding sites of Chlorine atom in the Glic Liganded-Closed-Channel Conformation, Mutant T25'A (pdb code 4lmj). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 5 binding sites of Chlorine where determined in the Glic Liganded-Closed-Channel Conformation, Mutant T25'A, PDB code: 4lmj:
Jump to Chlorine binding site number: 1; 2; 3; 4; 5;

Chlorine binding site 1 out of 5 in the Glic Liganded-Closed-Channel Conformation, Mutant T25'A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Glic Liganded-Closed-Channel Conformation, Mutant T25'A within 5.0Å range: probe atom residue distance (Å) B Occ E:Cl401 b:67.5 occ:1.00 OG E:SER219 3.4 53.5 1.0 CA E:SER219 3.5 50.0 1.0 N E:TYR220 3.5 47.4 1.0 CB E:SER219 3.8 48.7 1.0 C E:SER219 3.8 51.5 1.0 N E:GLU221 4.0 52.4 1.0 CB E:GLU221 4.5 56.3 1.0 CA E:TYR220 4.5 47.1 1.0 N E:SER219 4.7 48.8 1.0 O E:THR218 4.7 57.4 1.0 CB E:TYR220 4.8 52.2 1.0 C E:TYR220 4.8 49.9 1.0 O E:SER219 4.8 55.3 1.0 CA E:GLU221 4.9 51.2 1.0

Chlorine binding site 2 out of 5 in the Glic Liganded-Closed-Channel Conformation, Mutant T25'A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Glic Liganded-Closed-Channel Conformation, Mutant T25'A within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl402 b:66.5 occ:1.00 N B:TYR220 3.3 43.9 1.0 OG B:SER219 3.4 62.6 1.0 CA B:SER219 3.5 51.1 1.0 C B:SER219 3.7 47.3 1.0 N B:GLU221 3.7 44.0 1.0 CB B:SER219 3.9 55.6 1.0 CA B:TYR220 4.2 46.3 1.0 CB B:GLU221 4.3 53.9 1.0 CB B:TYR220 4.5 46.6 1.0 C B:TYR220 4.5 48.5 1.0 O B:SER219 4.7 49.2 1.0 CA B:GLU221 4.7 44.6 1.0 O B:THR218 4.7 54.8 1.0 N B:SER219 4.8 46.4 1.0

Chlorine binding site 3 out of 5 in the Glic Liganded-Closed-Channel Conformation, Mutant T25'A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Glic Liganded-Closed-Channel Conformation, Mutant T25'A within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl402 b:65.0 occ:1.00 N C:TYR220 3.0 43.1 1.0 CA C:SER219 3.6 43.0 1.0 C C:SER219 3.7 42.7 1.0 CB C:TYR220 3.9 48.6 1.0 CA C:TYR220 4.0 43.1 1.0 O C:THR218 4.0 59.9 1.0 N C:GLU221 4.2 51.2 1.0 OG C:SER219 4.2 55.0 1.0 CB C:SER219 4.4 50.4 1.0 N C:SER219 4.6 47.2 1.0 C C:TYR220 4.6 44.6 1.0 C C:THR218 4.7 55.5 1.0 O C:SER219 4.9 45.8 1.0

Chlorine binding site 4 out of 5 in the Glic Liganded-Closed-Channel Conformation, Mutant T25'A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Glic Liganded-Closed-Channel Conformation, Mutant T25'A within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl402 b:63.6 occ:1.00 N A:TYR220 3.3 46.0 1.0 N A:GLU221 3.8 43.9 1.0 CA A:SER219 4.0 52.5 1.0 C A:SER219 4.0 52.4 1.0 OG A:SER219 4.0 48.3 1.0 CA A:TYR220 4.1 46.7 1.0 CB A:TYR220 4.1 49.8 1.0 CB A:GLU221 4.4 63.9 1.0 C A:TYR220 4.5 43.5 1.0 CB A:SER219 4.5 46.1 1.0 CA A:GLU221 4.7 51.2 1.0 O A:THR218 4.9 55.7 1.0 O A:SER219 5.0 56.5 1.0

Chlorine binding site 5 out of 5 in the Glic Liganded-Closed-Channel Conformation, Mutant T25'A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 5 of Glic Liganded-Closed-Channel Conformation, Mutant T25'A within 5.0Å range: probe atom residue distance (Å) B Occ D:Cl402 b:63.6 occ:1.00 N D:TYR220 3.0 40.2 1.0 CA D:SER219 3.5 50.5 1.0 C D:SER219 3.6 48.7 1.0 OG D:SER219 3.7 49.8 1.0 N D:GLU221 3.7 47.5 1.0 CA D:TYR220 4.0 43.3 1.0 CB D:SER219 4.0 45.8 1.0 CB D:TYR220 4.1 52.3 1.0 C D:TYR220 4.4 49.4 1.0 O D:THR218 4.4 57.2 1.0 CB D:GLU221 4.5 54.7 1.0 O D:SER219 4.6 50.0 1.0 N D:SER219 4.7 47.5 1.0 CA D:GLU221 4.8 49.9 1.0 C D:THR218 5.0 49.6 1.0

G.Gonzalez-Gutierrez, L.G.Cuello, S.K.Nair, C.Grosman. Gating of the Proton-Gated Ion Channel From Gloeobacter Violaceus at pH 4 As Revealed By X-Ray Crystallography. Proc.Natl.Acad.Sci.Usa V. 110 18716 2013.
ISSN: ISSN 0027-8424
PubMed: 24167270
DOI: 10.1073/PNAS.1313156110