Chlorine in PDB 4mez: Crystal Structure of M68L/M69T Double Mutant Tem-1

All present enzymatic activity of Crystal Structure of M68L/M69T Double Mutant Tem-1:
3.5.2.6;

The structure of Crystal Structure of M68L/M69T Double Mutant Tem-1, PDB code: 4mez was solved by J.Park, S.Gobeil, J.N.Pelletier, A.M.Berghuis, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	73.26 / 2.05
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	34.333, 55.071, 77.681, 70.83, 81.22, 71.84
R / Rfree (%)	19.2 / 24.8

The binding sites of Chlorine atom in the Crystal Structure of M68L/M69T Double Mutant Tem-1 (pdb code 4mez). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of M68L/M69T Double Mutant Tem-1, PDB code: 4mez:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Crystal Structure of M68L/M69T Double Mutant Tem-1


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of M68L/M69T Double Mutant Tem-1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl301 b:41.8 occ:1.00 O A:HOH427 3.6 11.9 1.0 NH1 A:ARG93 3.7 10.6 1.0 NH2 A:ARG93 3.8 10.4 1.0 CG2 A:THR141 4.1 8.2 1.0 CZ A:ARG93 4.2 11.4 1.0 CA A:THR141 4.2 9.5 1.0 CG2 A:THR140 4.5 7.7 1.0 N A:THR141 4.6 8.6 1.0 CB A:THR141 4.6 9.3 1.0 O A:HOH463 4.7 11.6 1.0 CD1 A:ILE95 4.8 8.9 1.0 C A:THR140 4.9 8.6 1.0 O A:THR140 4.9 9.2 1.0

Chlorine binding site 2 out of 2 in the Crystal Structure of M68L/M69T Double Mutant Tem-1


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of M68L/M69T Double Mutant Tem-1 within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl301 b:41.1 occ:1.00 NH1 B:ARG93 3.5 9.7 1.0 O B:HOH492 3.5 12.4 1.0 NH2 B:ARG93 3.9 10.4 1.0 CG2 B:THR141 4.1 7.6 1.0 CZ B:ARG93 4.2 10.2 1.0 O B:HOH535 4.3 22.0 1.0 O B:HOH491 4.4 12.4 1.0 CA B:THR141 4.5 8.9 1.0 CG2 B:THR140 4.6 8.0 1.0 CD1 B:ILE95 4.7 7.3 1.0 CB B:THR141 4.7 8.7 1.0 N B:THR141 4.8 8.6 1.0 CG1 B:ILE95 5.0 7.8 1.0

S.Gobeil, J.Park, A.M.Berghuis, J.N.Pelletier. Structure/Function Correlation of Tem-1/Pse-4 Beta-Lactamase Chimeras and Their Deconvoluted Mutant To Be Published.