Chlorine in PDB 4ntx: Structure of Acid-Sensing Ion Channel in Complex with Snake Toxin and Amiloride

The structure of Structure of Acid-Sensing Ion Channel in Complex with Snake Toxin and Amiloride, PDB code: 4ntx was solved by I.Baconguis, C.J.Bohlen, A.Goehring, D.Julius, E.Gouaux, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.00 / 2.27
Space group	H 3
Cell size a, b, c (Å), α, β, γ (°)	151.560, 151.560, 123.200, 90.00, 90.00, 120.00
R / Rfree (%)	20.6 / 23.3

The structure of Structure of Acid-Sensing Ion Channel in Complex with Snake Toxin and Amiloride also contains other interesting chemical elements:

Sodium

(Na)

3 atoms

The binding sites of Chlorine atom in the Structure of Acid-Sensing Ion Channel in Complex with Snake Toxin and Amiloride (pdb code 4ntx). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the Structure of Acid-Sensing Ion Channel in Complex with Snake Toxin and Amiloride, PDB code: 4ntx:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in the Structure of Acid-Sensing Ion Channel in Complex with Snake Toxin and Amiloride


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Structure of Acid-Sensing Ion Channel in Complex with Snake Toxin and Amiloride within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl503 b:59.9 occ:1.00 OE2 A:GLU314 3.2 48.0 1.0 NE A:ARG310 3.3 39.7 1.0 NH1 A:ARG310 3.4 40.4 1.0 CG A:GLU314 3.7 42.9 1.0 CZ A:ARG310 3.8 45.5 1.0 CD A:GLU314 3.9 45.7 1.0 CB A:ARG310 4.2 41.1 1.0 CG2 A:VAL353 4.3 49.6 1.0 CG A:ARG310 4.4 44.7 1.0 CD A:ARG310 4.4 41.5 1.0 CG A:LEU352 4.4 48.9 1.0 CA A:VAL353 4.5 51.0 1.0 O A:ARG310 4.5 42.1 1.0 CD2 A:LEU352 4.5 44.6 1.0 O A:LEU352 4.8 55.3 1.0 N A:VAL353 4.8 50.7 1.0 CA A:ARG310 4.8 43.3 1.0 C A:LEU352 4.9 49.8 1.0 CB A:CYS360 4.9 52.8 1.0 CB A:VAL353 4.9 50.3 1.0

Chlorine binding site 2 out of 4 in the Structure of Acid-Sensing Ion Channel in Complex with Snake Toxin and Amiloride


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Structure of Acid-Sensing Ion Channel in Complex with Snake Toxin and Amiloride within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl507 b:0.3 occ:1.00 CL1 A:AMR507 0.0 0.3 1.0 C5 A:AMR507 1.7 0.5 1.0 N1 A:AMR507 2.7 0.7 1.0 C4 A:AMR507 2.7 0.7 1.0 N2 A:AMR507 3.0 100.0 1.0 CA A:GLY432 3.7 80.9 1.0 C1 A:AMR507 3.9 0.1 1.0 N3 A:AMR507 4.0 0.3 1.0 O A:GLY432 4.4 82.6 1.0 C A:GLY432 4.4 81.3 1.0 C2 A:AMR507 4.5 1.0 1.0 N A:GLY432 4.8 79.1 1.0

Chlorine binding site 3 out of 4 in the Structure of Acid-Sensing Ion Channel in Complex with Snake Toxin and Amiloride


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Structure of Acid-Sensing Ion Channel in Complex with Snake Toxin and Amiloride within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl508 b:86.3 occ:1.00 CL1 A:AMR508 0.0 86.3 1.0 C5 A:AMR508 1.7 68.5 1.0 C4 A:AMR508 2.7 65.0 1.0 N1 A:AMR508 2.7 69.9 1.0 N2 A:AMR508 2.9 65.2 1.0 O7 A:P6G506 3.1 69.7 1.0 C6 A:P6G506 3.3 62.7 1.0 C8 A:P6G506 3.4 70.5 1.0 O4 A:P6G506 3.5 58.9 1.0 C9 A:P6G506 3.7 67.5 1.0 N3 A:AMR508 3.9 67.8 1.0 C1 A:AMR508 3.9 66.0 1.0 C5 A:P6G506 4.0 53.8 1.0 O1 A:P6G506 4.4 50.9 1.0 C2 A:AMR508 4.4 68.5 1.0 N6 A:AMR509 4.6 66.7 1.0 C3 A:P6G506 4.6 51.9 1.0 C2 A:P6G506 4.8 49.1 1.0

Chlorine binding site 4 out of 4 in the Structure of Acid-Sensing Ion Channel in Complex with Snake Toxin and Amiloride


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Structure of Acid-Sensing Ion Channel in Complex with Snake Toxin and Amiloride within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl509 b:75.1 occ:1.00 CL1 A:AMR509 0.0 75.1 1.0 C5 A:AMR509 1.7 74.9 1.0 N1 A:AMR509 2.6 70.8 1.0 C4 A:AMR509 2.7 75.7 1.0 N2 A:AMR509 3.0 70.2 1.0 O A:GLU236 3.3 54.2 1.0 C A:GLU236 3.4 48.3 1.0 CA A:THR237 3.5 45.4 1.0 N A:THR237 3.5 49.0 1.0 C A:THR237 3.6 45.3 1.0 O A:THR237 3.9 47.6 1.0 N6 A:AMR508 3.9 74.3 1.0 C1 A:AMR509 3.9 74.2 1.0 CB A:GLU236 4.0 48.3 1.0 OD1 A:ASP238 4.0 50.1 1.0 N3 A:AMR509 4.0 78.1 1.0 N A:ASP238 4.1 42.0 1.0 C6 A:AMR508 4.1 74.3 1.0 N5 A:AMR508 4.2 70.4 1.0 CA A:GLU236 4.3 47.3 1.0 C2 A:AMR509 4.5 78.0 1.0 N7 A:AMR508 4.9 78.7 1.0 CA A:ASP238 4.9 43.5 1.0 CB A:THR237 5.0 45.3 1.0 N5 A:AMR509 5.0 69.9 1.0

I.Baconguis, C.J.Bohlen, A.Goehring, D.Julius, E.Gouaux. X-Ray Structure of Acid-Sensing Ion Channel 1-Snake Toxin Complex Reveals Open State of A Na(+)-Selective Channel. Cell(Cambridge,Mass.) V. 156 717 2014.
ISSN: ISSN 0092-8674
PubMed: 24507937
DOI: 10.1016/J.CELL.2014.01.011