Chlorine in PDB 4nwh: Lysozyme Under 30 Bar Pressure of Xenon

All present enzymatic activity of Lysozyme Under 30 Bar Pressure of Xenon:
3.2.1.17;

The structure of Lysozyme Under 30 Bar Pressure of Xenon, PDB code: 4nwh was solved by N.Colloc'h, T.Prange, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.65
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	79.306, 79.306, 38.072, 90.00, 90.00, 90.00
R / Rfree (%)	17.3 / 20.8

The structure of Lysozyme Under 30 Bar Pressure of Xenon also contains other interesting chemical elements:

Xenon	(Xe)	2 atoms
Sodium	(Na)	1 atom

The binding sites of Chlorine atom in the Lysozyme Under 30 Bar Pressure of Xenon (pdb code 4nwh). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 3 binding sites of Chlorine where determined in the Lysozyme Under 30 Bar Pressure of Xenon, PDB code: 4nwh:
Jump to Chlorine binding site number: 1; 2; 3;

Chlorine binding site 1 out of 3 in the Lysozyme Under 30 Bar Pressure of Xenon


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Lysozyme Under 30 Bar Pressure of Xenon within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl204 b:25.9 occ:1.00 ND2 A:ASN113 3.2 14.1 1.0 CB A:ASN113 3.6 14.0 1.0 CG A:ASN113 3.9 14.3 1.0 CG1 A:VAL109 3.9 17.4 1.0 O A:VAL109 4.0 13.6 1.0 CA A:ALA110 4.1 12.6 1.0 C A:VAL109 4.3 13.6 1.0 N A:ALA110 4.4 12.8 1.0 CD A:ARG114 4.5 15.6 1.0 CG A:ARG114 4.6 15.8 1.0 CB A:ALA110 4.7 13.9 1.0 CA A:ASN113 4.9 13.9 1.0 CB A:VAL109 5.0 16.3 1.0

Chlorine binding site 2 out of 3 in the Lysozyme Under 30 Bar Pressure of Xenon


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Lysozyme Under 30 Bar Pressure of Xenon within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl205 b:32.3 occ:1.00 OG A:SER24 3.1 19.0 1.0 N A:GLY26 3.1 12.7 1.0 CB A:SER24 3.5 16.6 1.0 CA A:GLY26 3.5 12.0 1.0 CA A:GLN121 3.7 23.3 1.0 CD1 A:ILE124 3.8 22.4 1.0 N A:GLN121 4.1 21.7 1.0 N A:LEU25 4.2 14.3 1.0 CB A:GLN121 4.2 27.6 1.0 CG1 A:ILE124 4.3 21.0 1.0 C A:LEU25 4.3 13.2 1.0 C A:SER24 4.3 14.7 1.0 O A:VAL120 4.3 19.7 1.0 CG A:GLN121 4.3 33.7 1.0 C A:VAL120 4.4 21.3 1.0 CA A:SER24 4.5 14.8 1.0 CD A:GLN121 4.5 39.7 1.0 CG2 A:VAL120 4.5 21.4 1.0 CA A:LEU25 4.6 14.2 1.0 NE2 A:GLN121 4.6 41.5 1.0 C A:GLY26 4.6 11.1 1.0 O A:SER24 4.7 13.3 1.0 N A:ASN27 4.8 10.9 1.0 CB A:LEU25 4.9 15.7 1.0 C A:GLN121 4.9 21.3 1.0

Chlorine binding site 3 out of 3 in the Lysozyme Under 30 Bar Pressure of Xenon


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Lysozyme Under 30 Bar Pressure of Xenon within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl206 b:29.7 occ:1.00 OG1 A:THR43 2.7 13.8 1.0 O A:GLN41 2.7 13.3 1.0 N A:THR43 3.2 11.6 1.0 CB A:THR43 3.6 12.9 1.0 CG2 A:THR43 3.7 12.7 1.0 C A:ALA42 3.8 11.3 1.0 C A:GLN41 3.8 12.1 1.0 CA A:THR43 3.9 12.5 1.0 CA A:ALA42 4.0 11.3 1.0 N A:ALA42 4.4 10.9 1.0 O A:THR43 4.4 15.6 1.0 C A:THR43 4.6 13.1 1.0 O A:ALA42 4.7 11.3 1.0

J.H.Abraini, G.Marassio, H.N.David, B.Vallone, T.Prange, N.Colloc'h. Crystallographic Studies with Xenon and Nitrous Oxide Provide Evidence For Protein-Dependent Processes in the Mechanisms of General Anesthesia Anesthesiology 2014.
ISSN: ESSN 1528-1175
PubMed: 25211169
DOI: 10.1097/ALN.0000000000000435