Chlorine in PDB 4r33: X-Ray Structure of the Tryptophan Lyase Nosl with Tryptophan and S- Adenosyl-L-Homocysteine Bound

The structure of X-Ray Structure of the Tryptophan Lyase Nosl with Tryptophan and S- Adenosyl-L-Homocysteine Bound, PDB code: 4r33 was solved by Y.Nicolet, L.Zeppieri, P.Amara, J.-C.Fontecilla-Camps, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.23 / 1.78
Space group	P 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	94.720, 47.230, 114.360, 90.00, 108.71, 90.00
R / Rfree (%)	15.9 / 18.1

The structure of X-Ray Structure of the Tryptophan Lyase Nosl with Tryptophan and S- Adenosyl-L-Homocysteine Bound also contains other interesting chemical elements:

Potassium	(K)	2 atoms
Iron	(Fe)	8 atoms
Sodium	(Na)	1 atom

The binding sites of Chlorine atom in the X-Ray Structure of the Tryptophan Lyase Nosl with Tryptophan and S- Adenosyl-L-Homocysteine Bound (pdb code 4r33). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the X-Ray Structure of the Tryptophan Lyase Nosl with Tryptophan and S- Adenosyl-L-Homocysteine Bound, PDB code: 4r33:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the X-Ray Structure of the Tryptophan Lyase Nosl with Tryptophan and S- Adenosyl-L-Homocysteine Bound


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of X-Ray Structure of the Tryptophan Lyase Nosl with Tryptophan and S- Adenosyl-L-Homocysteine Bound within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl509 b:63.5 occ:0.89 O B:HOH854 2.3 52.8 1.0 O B:LEU167 3.0 23.7 1.0 NA B:NA507 3.0 36.8 1.0 CD2 B:LEU167 3.9 23.0 1.0 C B:LEU167 4.0 19.2 1.0 CE1 B:TYR129 4.1 23.0 1.0 CZ B:TYR129 4.1 23.2 1.0 O B:HOH1042 4.2 51.8 1.0 CA B:LEU167 4.2 18.2 1.0 NE2 B:HIS130 4.3 25.0 1.0 CB B:LEU167 4.3 18.4 1.0 CD1 B:TYR129 4.4 19.8 1.0 CE2 B:TYR129 4.4 20.9 1.0 OH B:TYR129 4.5 24.3 1.0 CD2 B:TYR129 4.7 18.1 1.0 CG B:TYR129 4.7 15.9 1.0 CE1 B:HIS130 4.7 25.9 1.0 CG B:LEU167 4.8 24.3 1.0 OE1 B:GLU126 4.9 45.2 1.0 O B:HOH932 4.9 47.2 1.0

Chlorine binding site 2 out of 2 in the X-Ray Structure of the Tryptophan Lyase Nosl with Tryptophan and S- Adenosyl-L-Homocysteine Bound


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of X-Ray Structure of the Tryptophan Lyase Nosl with Tryptophan and S- Adenosyl-L-Homocysteine Bound within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl510 b:57.5 occ:1.00 O B:HOH1000 2.7 45.4 1.0 OE2 B:GLU48 3.3 39.1 1.0 OE1 B:GLU48 3.5 41.4 1.0 CD B:GLU48 3.8 38.5 1.0 N B:ASP33 3.8 36.7 1.0 CB B:ASP33 4.0 41.6 1.0 O B:ASP33 4.2 37.0 1.0 CA B:ASP33 4.4 39.2 1.0 CG2 B:VAL37 4.5 31.0 1.0 CB B:ALA36 4.6 41.9 1.0 O B:HOH920 4.7 50.2 1.0 C B:ASP33 4.7 37.0 1.0 CA B:VAL32 4.8 33.4 1.0 C B:VAL32 4.8 36.7 1.0 CG1 B:VAL32 4.8 31.1 1.0 CB B:ALA47 4.9 45.3 1.0 N B:ALA47 4.9 42.4 1.0

Y.Nicolet, L.Zeppieri, P.Amara, J.C.Fontecilla-Camps. Crystal Structure of Tryptophan Lyase (Nosl): Evidence For Radical Formation at the Amino Group of Tryptophan. Angew.Chem.Int.Ed.Engl. V. 53 11840 2014.
ISSN: ISSN 1433-7851
PubMed: 25196319
DOI: 10.1002/ANIE.201407320