Chlorine in PDB 4tmt: Translation Initiation Factor EIF5B (517-858) Mutant D533A From C. Thermophilum, Bound to Gtpgammas

The structure of Translation Initiation Factor EIF5B (517-858) Mutant D533A From C. Thermophilum, Bound to Gtpgammas, PDB code: 4tmt was solved by B.Kuhle, F.Ficner, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.37 / 1.58
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	55.410, 115.910, 66.090, 90.00, 101.37, 90.00
R / Rfree (%)	16.9 / 19.4

The structure of Translation Initiation Factor EIF5B (517-858) Mutant D533A From C. Thermophilum, Bound to Gtpgammas also contains other interesting chemical elements:

Magnesium

(Mg)

2 atoms

The binding sites of Chlorine atom in the Translation Initiation Factor EIF5B (517-858) Mutant D533A From C. Thermophilum, Bound to Gtpgammas (pdb code 4tmt). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Translation Initiation Factor EIF5B (517-858) Mutant D533A From C. Thermophilum, Bound to Gtpgammas, PDB code: 4tmt:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Translation Initiation Factor EIF5B (517-858) Mutant D533A From C. Thermophilum, Bound to Gtpgammas


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Translation Initiation Factor EIF5B (517-858) Mutant D533A From C. Thermophilum, Bound to Gtpgammas within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl908 b:45.5 occ:1.00 O A:HOH1267 2.6 41.8 1.0 N A:PHE643 3.1 12.7 1.0 NH1 A:ARG737 3.1 34.3 1.0 O A:PHE643 3.4 15.1 1.0 NH2 A:ARG737 3.5 32.4 1.0 CA A:PRO642 3.6 14.9 1.0 CZ A:ARG737 3.8 30.9 1.0 CD2 A:PHE643 3.9 15.6 1.0 C A:PRO642 3.9 15.2 1.0 CA A:PHE643 4.2 12.0 1.0 CE2 A:PHE643 4.2 17.3 1.0 O A:THR641 4.2 17.5 1.0 C A:PHE643 4.2 12.9 1.0 O A:HOH1092 4.2 32.7 1.0 CG A:PHE643 4.4 14.0 1.0 CB A:PRO642 4.5 16.4 1.0 O A:HOH1071 4.6 21.2 1.0 O A:HOH1307 4.6 41.8 1.0 N A:PRO642 4.7 15.0 1.0 CB A:PHE643 4.8 13.9 1.0 CG2 A:VAL710 4.9 23.5 1.0 C A:THR641 4.9 15.3 1.0 O A:HOH1063 4.9 45.2 1.0

Chlorine binding site 2 out of 2 in the Translation Initiation Factor EIF5B (517-858) Mutant D533A From C. Thermophilum, Bound to Gtpgammas


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Translation Initiation Factor EIF5B (517-858) Mutant D533A From C. Thermophilum, Bound to Gtpgammas within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl911 b:48.0 occ:1.00 NZ B:LYS573 3.1 23.0 1.0 O B:ARG544 3.4 21.3 1.0 O B:ILE543 3.6 19.2 1.0 CG B:GLN545 3.7 27.9 1.0 CE B:LYS573 3.8 20.5 1.0 C B:ARG544 3.9 18.8 1.0 CE B:MET802 3.9 39.4 1.0 CD B:GLN545 4.0 37.4 1.0 CB B:GLN545 4.2 24.1 1.0 OE1 B:GLN545 4.4 39.0 1.0 CA B:ARG544 4.4 15.1 1.0 SD B:MET802 4.4 32.9 1.0 O B:HOH1276 4.5 24.6 1.0 N B:GLN545 4.5 19.2 1.0 NE2 B:GLN545 4.5 41.7 1.0 C B:ILE543 4.7 18.3 1.0 O A:HOH1346 4.8 42.2 0.5 O B:HOH1356 4.8 51.2 1.0 O B:HOH1277 5.0 25.2 1.0

B.Kuhle, R.Ficner. A Monovalent Cation Acts As Structural and Catalytic Cofactor in Translational Gtpases. Embo J. 2014.
ISSN: ESSN 1460-2075
PubMed: 25225612
DOI: 10.15252/EMBJ.201488517