Chlorine in PDB 4tmz: Translation Initiation Factor EIF5B (517-858) From C. Thermophilum, Bound to Gtpgammas and Potassium

The structure of Translation Initiation Factor EIF5B (517-858) From C. Thermophilum, Bound to Gtpgammas and Potassium, PDB code: 4tmz was solved by B.Kuhle, F.Ficner, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.68 / 2.28
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	116.130, 116.130, 120.270, 90.00, 90.00, 90.00
R / Rfree (%)	20 / 23.9

The structure of Translation Initiation Factor EIF5B (517-858) From C. Thermophilum, Bound to Gtpgammas and Potassium also contains other interesting chemical elements:

Magnesium	(Mg)	2 atoms
Potassium	(K)	2 atoms

The binding sites of Chlorine atom in the Translation Initiation Factor EIF5B (517-858) From C. Thermophilum, Bound to Gtpgammas and Potassium (pdb code 4tmz). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Translation Initiation Factor EIF5B (517-858) From C. Thermophilum, Bound to Gtpgammas and Potassium, PDB code: 4tmz:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Translation Initiation Factor EIF5B (517-858) From C. Thermophilum, Bound to Gtpgammas and Potassium


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Translation Initiation Factor EIF5B (517-858) From C. Thermophilum, Bound to Gtpgammas and Potassium within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl905 b:76.1 occ:1.00 NH1 B:ARG737 3.2 52.9 0.5 N B:PHE643 3.3 37.2 1.0 O B:HOH1102 3.6 58.1 1.0 O B:PHE643 3.6 46.5 1.0 CD2 B:PHE643 3.7 36.4 1.0 CA B:PRO642 3.7 42.3 1.0 NH2 B:ARG737 3.8 54.3 0.5 CZ B:ARG737 3.9 54.4 0.5 CE2 B:PHE643 4.0 42.5 1.0 C B:PRO642 4.0 42.1 1.0 CA B:PHE643 4.3 39.4 1.0 O B:THR641 4.4 41.3 1.0 C B:PHE643 4.4 45.8 1.0 CG B:PHE643 4.4 34.4 1.0 O B:HOH1037 4.5 47.0 1.0 CG2 B:VAL710 4.6 46.5 1.0 CB B:PRO642 4.6 45.4 1.0 CZ B:PHE643 4.8 42.2 1.0 N B:PRO642 4.8 45.9 1.0 CB B:PHE643 4.9 35.7 1.0 C B:THR641 5.0 44.1 1.0

Chlorine binding site 2 out of 2 in the Translation Initiation Factor EIF5B (517-858) From C. Thermophilum, Bound to Gtpgammas and Potassium


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Translation Initiation Factor EIF5B (517-858) From C. Thermophilum, Bound to Gtpgammas and Potassium within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl906 b:71.8 occ:1.00 N B:GLU804 3.3 52.7 1.0 CD B:ARG803 3.5 57.2 0.5 CD B:ARG803 3.7 57.5 0.5 CG2 B:VAL548 3.7 38.0 1.0 CB B:GLU804 3.8 58.6 1.0 CG1 B:ILE807 3.9 86.3 1.0 CA B:ARG803 3.9 49.1 0.5 CA B:ARG803 3.9 49.1 0.5 CG2 B:THR546 4.0 48.1 1.0 C B:ARG803 4.1 47.0 0.5 C B:ARG803 4.1 47.0 0.5 CB B:ARG803 4.1 50.6 0.5 CA B:GLU804 4.2 55.5 1.0 CG B:GLU804 4.2 65.6 1.0 CB B:ARG803 4.2 50.4 0.5 CB B:ILE807 4.2 86.8 1.0 CB B:THR546 4.3 50.9 1.0 OG1 B:THR546 4.4 47.1 1.0 CG B:ARG803 4.5 53.5 0.5 CG2 B:ILE807 4.5 92.2 1.0 NE B:ARG803 4.5 62.2 0.5 NE B:ARG544 4.5 38.8 1.0 CG B:ARG803 4.6 53.5 0.5 CD B:ARG544 4.7 40.4 1.0 NE B:ARG803 4.8 62.4 0.5 O B:HOH1046 4.8 58.9 1.0 O B:MET802 4.9 57.8 1.0 O B:GLU804 5.0 61.5 1.0

B.Kuhle, R.Ficner. A Monovalent Cation Acts As Structural and Catalytic Cofactor in Translational Gtpases. Embo J. 2014.
ISSN: ESSN 1460-2075
PubMed: 25225612
DOI: 10.15252/EMBJ.201488517