Chlorine in PDB 4uhm: Characterization of A Novel Transaminase From Pseudomonas Sp. Strain Aac

All present enzymatic activity of Characterization of A Novel Transaminase From Pseudomonas Sp. Strain Aac:
2.6.1.18;

The structure of Characterization of A Novel Transaminase From Pseudomonas Sp. Strain Aac, PDB code: 4uhm was solved by M.Wilding, T.S.Peat, J.Newman, C.Scott, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	89.47 / 1.33
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	62.877, 119.971, 134.310, 90.00, 90.00, 90.00
R / Rfree (%)	10.7 / 12.644

The structure of Characterization of A Novel Transaminase From Pseudomonas Sp. Strain Aac also contains other interesting chemical elements:

Magnesium

(Mg)

2 atoms

The binding sites of Chlorine atom in the Characterization of A Novel Transaminase From Pseudomonas Sp. Strain Aac (pdb code 4uhm). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Characterization of A Novel Transaminase From Pseudomonas Sp. Strain Aac, PDB code: 4uhm:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Characterization of A Novel Transaminase From Pseudomonas Sp. Strain Aac


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Characterization of A Novel Transaminase From Pseudomonas Sp. Strain Aac within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1459 b:27.9 occ:0.80 OG A:SER231 2.6 14.0 0.3 O A:HOH2276 2.7 22.4 0.5 O A:HOH2263 2.8 35.4 1.0 ND2 A:ASN169 3.0 8.1 1.0 CD1 A:ILE166 3.6 9.4 1.0 CD1 A:TYR153 3.7 6.7 1.0 CE1 A:TYR153 3.7 6.7 1.0 O A:HOH2542 3.8 35.8 1.0 OD1 A:ASN169 3.8 9.9 1.0 CG A:ASN169 3.8 7.7 1.0 CB A:SER231 3.9 10.3 0.7 CB A:SER231 3.9 10.8 0.3 O A:HOH2545 3.9 26.7 1.0 CG A:TYR153 4.2 6.1 1.0 CZ A:TYR153 4.3 6.8 1.0 O A:HOH2365 4.4 15.6 1.0 OG A:SER231 4.5 13.0 0.7 O A:SER231 4.7 9.7 0.7 CZ A:PHE24 4.8 9.8 1.0 O A:SER231 4.8 9.5 0.3 CD2 A:TYR153 4.8 6.8 1.0 CE2 A:TYR153 4.8 7.4 1.0 CG1 A:ILE166 4.9 8.3 1.0 CG2 A:ILE166 4.9 8.1 1.0 OH A:TYR153 4.9 9.5 1.0 C A:SER231 4.9 8.3 0.7 CB A:TYR153 4.9 6.1 1.0 C A:SER231 4.9 8.7 0.3 CA A:SER231 5.0 8.9 0.7 CB A:ILE166 5.0 7.5 1.0

Chlorine binding site 2 out of 2 in the Characterization of A Novel Transaminase From Pseudomonas Sp. Strain Aac


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Characterization of A Novel Transaminase From Pseudomonas Sp. Strain Aac within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1460 b:13.7 occ:0.70 O A:HOH2370 3.1 11.9 1.0 O A:EOH1452 3.1 20.5 0.8 C1 A:EOH1452 3.1 15.0 0.8 N A:LEU242 3.2 6.6 1.0 O A:HOH2368 3.2 12.9 1.0 O A:PRO238 3.4 8.4 1.0 CB A:LEU242 3.6 7.3 1.0 CG A:LEU242 3.6 7.5 1.0 N A:TYR241 3.7 7.0 1.0 CB A:PRO237 3.8 8.7 1.0 CD1 A:LEU242 3.9 8.1 1.0 CG A:PRO237 4.0 8.4 1.0 CA A:LEU242 4.0 7.2 1.0 N A:GLY240 4.1 8.3 1.0 CD1 A:PHE277 4.1 11.1 1.0 CB A:TYR241 4.1 6.2 1.0 C A:TYR241 4.2 6.5 1.0 CA A:TYR241 4.2 6.3 1.0 C2 A:EOH1452 4.3 13.9 0.8 C A:GLY240 4.4 7.3 1.0 C A:PRO238 4.4 8.3 1.0 O A:PRO237 4.5 8.2 1.0 CG A:PHE277 4.6 9.9 1.0 CA A:GLY240 4.7 8.2 1.0 CB A:PHE277 4.7 9.3 1.0 C A:LYS239 4.7 9.1 0.5 C A:LYS239 4.7 9.2 0.5 C A:PRO237 4.7 7.9 1.0 CE1 A:PHE277 4.7 11.7 1.0 N A:GLN243 4.8 7.2 0.5 CA A:LYS239 4.8 9.4 0.5 N A:GLN243 4.8 7.1 0.5 CA A:LYS239 4.8 9.0 0.5 CA A:PRO237 4.9 7.7 1.0 C A:LEU242 5.0 7.0 1.0 CD2 A:TYR241 5.0 6.3 1.0

M.Wilding, T.S.Peat, J.Newman, C.Scott. A Beta-Alanine Catabolism Pathway Containing A Highly Promiscuous Omega-Transaminase in the 12-Aminododecanate- Degrading Pseudomonas Sp. Strain Aac. Appl.Environ.Microbiol. V. 82 3846 2016.
ISSN: ISSN 0099-2240
PubMed: 27107110
DOI: 10.1128/AEM.00665-16