Chlorine in PDB 4zf4: Crystal Structure of Green Fluorescent Protein (Gfp); S65T, Y66(CL1Y), H148D; Circular Permutant (50-51)

The structure of Crystal Structure of Green Fluorescent Protein (Gfp); S65T, Y66(CL1Y), H148D; Circular Permutant (50-51), PDB code: 4zf4 was solved by L.M.Oltrogge, S.G.Boxer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	36.34 / 1.82
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	51.840, 68.590, 60.880, 90.00, 100.50, 90.00
R / Rfree (%)	19.8 / 24.6

The binding sites of Chlorine atom in the Crystal Structure of Green Fluorescent Protein (Gfp); S65T, Y66(CL1Y), H148D; Circular Permutant (50-51) (pdb code 4zf4). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of Green Fluorescent Protein (Gfp); S65T, Y66(CL1Y), H148D; Circular Permutant (50-51), PDB code: 4zf4:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Crystal Structure of Green Fluorescent Protein (Gfp); S65T, Y66(CL1Y), H148D; Circular Permutant (50-51)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of Green Fluorescent Protein (Gfp); S65T, Y66(CL1Y), H148D; Circular Permutant (50-51) within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl15 b:27.7 occ:1.00 CL1 A:4NT15 0.0 27.7 1.0 CE1 A:4NT15 1.8 19.5 1.0 OG1 A:THR155 2.7 18.4 1.0 CD1 A:4NT15 2.7 19.5 1.0 CZ A:4NT15 2.8 20.1 1.0 OH A:4NT15 3.0 23.7 1.0 CD A:GLU172 3.4 22.6 1.0 O A:HOH319 3.5 14.6 1.0 OE2 A:GLU172 3.5 24.8 1.0 OE1 A:GLU172 3.6 19.8 1.0 CE2 A:PHE95 3.6 18.4 1.0 CB A:THR153 3.8 14.8 1.0 OG1 A:THR153 3.9 20.3 1.0 CG A:GLU172 3.9 19.3 1.0 CB A:THR155 4.0 15.2 1.0 CD2 A:PHE95 4.0 19.2 1.0 CE2 A:4NT15 4.1 17.7 1.0 CG1 A:VAL11 4.1 17.1 1.0 CG2 A:4NT15 4.1 18.8 1.0 CG2 A:THR153 4.3 18.5 1.0 CG2 A:THR155 4.4 17.7 1.0 CZ A:PHE95 4.6 17.8 1.0 CD2 A:4NT15 4.6 19.5 1.0 OG1 A:4NT15 4.9 16.8 1.0

Chlorine binding site 2 out of 2 in the Crystal Structure of Green Fluorescent Protein (Gfp); S65T, Y66(CL1Y), H148D; Circular Permutant (50-51)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of Green Fluorescent Protein (Gfp); S65T, Y66(CL1Y), H148D; Circular Permutant (50-51) within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl15 b:30.5 occ:1.00 CL1 B:4NT15 0.0 30.5 1.0 CE1 B:4NT15 1.7 22.9 1.0 CZ B:4NT15 2.7 19.9 1.0 CD1 B:4NT15 2.8 20.5 1.0 OG1 B:THR155 2.9 20.4 1.0 OH B:4NT15 2.9 22.2 1.0 O B:HOH312 3.5 19.7 1.0 CD B:GLU172 3.5 22.5 1.0 CE2 B:PHE95 3.6 20.6 1.0 OE2 B:GLU172 3.7 24.7 1.0 OE1 B:GLU172 3.7 20.3 1.0 OG1 B:THR153 3.8 22.1 1.0 CD2 B:PHE95 3.9 19.8 1.0 CB B:THR153 3.9 18.1 1.0 CG B:GLU172 4.0 21.5 1.0 CE2 B:4NT15 4.1 21.4 1.0 CB B:THR155 4.1 18.0 1.0 CG2 B:4NT15 4.2 20.8 1.0 CG1 B:VAL11 4.2 17.6 1.0 CG2 B:THR153 4.4 19.9 1.0 CZ B:PHE95 4.5 21.0 1.0 CG2 B:THR155 4.6 17.1 1.0 CD2 B:4NT15 4.6 21.6 1.0 OG1 B:4NT15 4.9 19.9 1.0 CG B:PHE95 5.0 21.6 1.0

L.M.Oltrogge, S.G.Boxer. Short Hydrogen Bonds and Proton Delocalization in Green Fluorescent Protein (Gfp). Acs Cent.Sci. V. 1 148 2015.
ISSN: ESSN 2374-7951
PubMed: 27162964
DOI: 10.1021/ACSCENTSCI.5B00160