Chlorine in PDB 4zow: Crystal Structure of E. Coli Multidrug Transporter Mdfa in Complex with Chloramphenicol

The structure of Crystal Structure of E. Coli Multidrug Transporter Mdfa in Complex with Chloramphenicol, PDB code: 4zow was solved by X.C.Zhang, J.Heng, Y.Zhao, X.Wang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.09 / 2.45
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	93.852, 63.449, 99.726, 90.00, 106.09, 90.00
R / Rfree (%)	20.4 / 23.5

The binding sites of Chlorine atom in the Crystal Structure of E. Coli Multidrug Transporter Mdfa in Complex with Chloramphenicol (pdb code 4zow). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of E. Coli Multidrug Transporter Mdfa in Complex with Chloramphenicol, PDB code: 4zow:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Crystal Structure of E. Coli Multidrug Transporter Mdfa in Complex with Chloramphenicol


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of E. Coli Multidrug Transporter Mdfa in Complex with Chloramphenicol within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl500 b:93.9 occ:1.00 CL1 A:CLM500 0.0 93.9 1.0 C1 A:CLM500 1.8 76.1 1.0 C2 A:CLM500 2.7 77.0 1.0 N2 A:CLM500 2.9 66.7 1.0 CL2 A:CLM500 2.9 74.8 1.0 CD1 A:TYR30 3.3 45.2 1.0 CE1 A:TYR30 3.7 51.5 1.0 CD2 A:LEU119 3.7 49.7 1.0 O2 A:CLM500 3.9 70.6 1.0 CA A:TYR30 4.1 38.9 1.0 CD1 A:LEU119 4.1 45.8 1.0 O A:THR29 4.1 39.1 1.0 CG A:TYR30 4.1 54.4 1.0 CE2 A:TYR61 4.3 40.0 1.0 CG A:LEU119 4.3 59.8 1.0 O5 A:CLM500 4.3 62.1 1.0 C3 A:CLM500 4.3 57.5 1.0 CB A:ASN33 4.4 33.8 1.0 CD2 A:LEU62 4.5 39.3 1.0 O4 A:CLM500 4.6 56.0 1.0 CB A:TYR30 4.6 44.2 1.0 O A:TYR30 4.6 43.9 1.0 CZ A:TYR30 4.7 64.5 1.0 CD2 A:TYR61 4.7 38.5 1.0 C A:THR29 4.8 40.6 1.0 N A:TYR30 4.8 33.7 1.0 C A:TYR30 4.9 40.7 1.0 C4 A:CLM500 5.0 65.3 1.0

Chlorine binding site 2 out of 2 in the Crystal Structure of E. Coli Multidrug Transporter Mdfa in Complex with Chloramphenicol


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of E. Coli Multidrug Transporter Mdfa in Complex with Chloramphenicol within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl500 b:74.8 occ:1.00 CL2 A:CLM500 0.0 74.8 1.0 C1 A:CLM500 1.8 76.1 1.0 C2 A:CLM500 2.7 77.0 1.0 CL1 A:CLM500 2.9 93.9 1.0 O2 A:CLM500 3.0 70.6 1.0 CE1 A:TYR30 3.8 51.5 1.0 CD2 A:LEU119 3.8 49.7 1.0 N2 A:CLM500 3.9 66.7 1.0 CZ A:TYR30 4.2 64.5 1.0 OH A:TYR30 4.2 61.4 1.0 CG A:LEU119 4.3 59.8 1.0 CD1 A:TYR30 4.4 45.2 1.0 CE A:MET358 4.5 51.4 1.0 CD2 A:LEU62 4.6 39.3 1.0 CD1 A:LEU119 5.0 45.8 1.0

J.Heng, Y.Zhao, M.Liu, Y.Liu, J.Fan, X.Wang, Y.Zhao, X.C.Zhang. Substrate-Bound Structure of the E. Coli Multidrug Resistance Transporter Mdfa Cell Res. V. 25 1060 2015.
ISSN: ISSN 1001-0602
PubMed: 26238402
DOI: 10.1038/CR.2015.94