Chlorine in PDB 5a8n: Crystal Structure of the Native Form of Beta-Glucanase SDGLUC5_26A From Saccharophagus Degradans

All present enzymatic activity of Crystal Structure of the Native Form of Beta-Glucanase SDGLUC5_26A From Saccharophagus Degradans:
3.2.1.73;

The structure of Crystal Structure of the Native Form of Beta-Glucanase SDGLUC5_26A From Saccharophagus Degradans, PDB code: 5a8n was solved by G.Sulzenbacher, M.Lafond, T.Freyd, B.Henrissat, R.M.Coutinho, J.G.Berrin, M.L.Garron, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	32.91 / 2.05
Space group	P 41 3 2
Cell size a, b, c (Å), α, β, γ (°)	143.341, 143.341, 143.341, 90.00, 90.00, 90.00
R / Rfree (%)	15.1 / 18

The structure of Crystal Structure of the Native Form of Beta-Glucanase SDGLUC5_26A From Saccharophagus Degradans also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Chlorine atom in the Crystal Structure of the Native Form of Beta-Glucanase SDGLUC5_26A From Saccharophagus Degradans (pdb code 5a8n). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of the Native Form of Beta-Glucanase SDGLUC5_26A From Saccharophagus Degradans, PDB code: 5a8n:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Crystal Structure of the Native Form of Beta-Glucanase SDGLUC5_26A From Saccharophagus Degradans


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of the Native Form of Beta-Glucanase SDGLUC5_26A From Saccharophagus Degradans within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1365 b:28.4 occ:1.00 O A:HOH2183 3.1 27.6 1.0 O A:HOH2195 3.1 37.8 1.0 N A:ASN231 3.2 25.2 1.0 NE A:ARG200 3.2 28.0 1.0 N A:THR192 3.3 24.6 1.0 CA A:THR191 3.6 25.1 1.0 OG1 A:THR191 3.7 25.1 1.0 CA A:ASN231 3.8 26.2 1.0 CB A:PHE230 3.8 27.5 1.0 OG1 A:THR192 3.8 24.9 1.0 NH2 A:ARG200 3.9 30.0 1.0 C A:THR191 4.0 25.1 1.0 CZ A:ARG200 4.0 28.7 1.0 CD A:ARG200 4.1 29.9 1.0 CB A:THR191 4.2 24.7 1.0 C A:PHE230 4.3 25.9 1.0 CA A:PHE230 4.4 25.8 1.0 CA A:THR192 4.4 24.2 1.0 CG A:PHE230 4.5 27.4 1.0 CB A:THR192 4.6 25.1 1.0 CB A:ASN231 4.7 26.6 1.0 N A:THR191 4.7 25.1 1.0 N A:MET193 4.7 25.2 1.0 O A:PRO190 4.7 24.0 1.0 CB A:ARG200 4.8 29.4 1.0 CG2 A:THR192 4.8 24.9 1.0 CD1 A:PHE230 4.9 28.0 1.0 N A:TRP232 5.0 25.3 1.0 C A:ASN231 5.0 25.9 1.0

Chlorine binding site 2 out of 2 in the Crystal Structure of the Native Form of Beta-Glucanase SDGLUC5_26A From Saccharophagus Degradans


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of the Native Form of Beta-Glucanase SDGLUC5_26A From Saccharophagus Degradans within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1366 b:62.4 occ:1.00 N A:ILE304 3.2 31.7 1.0 CG1 A:ILE304 3.7 37.4 1.0 CA A:HIS303 3.7 31.8 1.0 CB A:ILE304 3.9 35.3 1.0 C A:HIS303 3.9 31.6 1.0 CB A:HIS303 4.0 32.0 1.0 CA A:ILE304 4.1 32.1 1.0 CD1 A:ILE304 4.2 38.9 1.0 CE A:LYS307 4.4 37.5 1.0 NZ A:LYS307 4.6 40.5 1.0 O A:ILE304 4.7 30.7 1.0 C A:ILE304 5.0 30.7 1.0

M.Lafond, G.Sulzenbacher, T.Freyd, B.Henrissat, J.G.Berrin, M.L.Garron. The Quaternary Structure of A Glycoside Hydrolase Dictates Specificity Towards Beta-Glucans J.Biol.Chem. V. 291 7183 2016.
ISSN: ISSN 0021-9258
PubMed: 26755730
DOI: 10.1074/JBC.M115.695999