Chlorine in PDB 5a8q: Crystal Structure Beta-Glucanase SDGLUC5_26A From Saccharophagus Degradans in Complex with Tetrasaccharide A Obtained By Soaking

Enzymatic activity of Crystal Structure Beta-Glucanase SDGLUC5_26A From Saccharophagus Degradans in Complex with Tetrasaccharide A Obtained By Soaking

All present enzymatic activity of Crystal Structure Beta-Glucanase SDGLUC5_26A From Saccharophagus Degradans in Complex with Tetrasaccharide A Obtained By Soaking:
3.2.1.73;

Protein crystallography data

The structure of Crystal Structure Beta-Glucanase SDGLUC5_26A From Saccharophagus Degradans in Complex with Tetrasaccharide A Obtained By Soaking, PDB code: 5a8q was solved by G.Sulzenbacher, M.Lafond, T.Freyd, B.Henrissat, R.M.Coutinho, J.G.Berrin, M.L.Garron, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 47.95 / 1.90
Space group P 41 3 2
Cell size a, b, c (Å), α, β, γ (°) 143.702, 143.702, 143.702, 90.00, 90.00, 90.00
R / Rfree (%) 13.8 / 16.1

Other elements in 5a8q:

The structure of Crystal Structure Beta-Glucanase SDGLUC5_26A From Saccharophagus Degradans in Complex with Tetrasaccharide A Obtained By Soaking also contains other interesting chemical elements:

Magnesium (Mg) 1 atom

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Crystal Structure Beta-Glucanase SDGLUC5_26A From Saccharophagus Degradans in Complex with Tetrasaccharide A Obtained By Soaking (pdb code 5a8q). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure Beta-Glucanase SDGLUC5_26A From Saccharophagus Degradans in Complex with Tetrasaccharide A Obtained By Soaking, PDB code: 5a8q:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in 5a8q

Go back to Chlorine Binding Sites List in 5a8q
Chlorine binding site 1 out of 2 in the Crystal Structure Beta-Glucanase SDGLUC5_26A From Saccharophagus Degradans in Complex with Tetrasaccharide A Obtained By Soaking


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure Beta-Glucanase SDGLUC5_26A From Saccharophagus Degradans in Complex with Tetrasaccharide A Obtained By Soaking within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl1375

b:19.1
occ:1.00
O A:HOH2270 3.2 30.5 1.0
O A:HOH2257 3.2 21.0 1.0
N A:ASN231 3.2 16.0 1.0
NE A:ARG200 3.2 18.1 1.0
N A:THR192 3.3 14.1 1.0
CA A:THR191 3.6 14.2 1.0
OG1 A:THR191 3.7 14.8 1.0
CB A:PHE230 3.7 16.4 1.0
CA A:ASN231 3.8 16.9 1.0
OG1 A:THR192 3.8 14.2 1.0
NH2 A:ARG200 3.9 19.3 1.0
C A:THR191 4.0 14.4 1.0
CZ A:ARG200 4.0 18.9 1.0
CD A:ARG200 4.0 18.0 1.0
CB A:THR191 4.2 14.7 1.0
C A:PHE230 4.2 16.1 1.0
CA A:PHE230 4.4 15.8 1.0
CA A:THR192 4.4 14.3 1.0
CG A:PHE230 4.4 16.0 1.0
CB A:THR192 4.5 14.2 1.0
CB A:ASN231 4.6 17.6 1.0
N A:MET193 4.7 15.7 1.0
N A:THR191 4.7 14.2 1.0
CB A:ARG200 4.7 17.1 1.0
O A:PRO190 4.7 13.9 1.0
CG2 A:THR192 4.8 14.1 1.0
CD1 A:PHE230 4.9 15.9 1.0
C A:ASN231 5.0 16.5 1.0
C A:THR192 5.0 15.0 1.0

Chlorine binding site 2 out of 2 in 5a8q

Go back to Chlorine Binding Sites List in 5a8q
Chlorine binding site 2 out of 2 in the Crystal Structure Beta-Glucanase SDGLUC5_26A From Saccharophagus Degradans in Complex with Tetrasaccharide A Obtained By Soaking


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure Beta-Glucanase SDGLUC5_26A From Saccharophagus Degradans in Complex with Tetrasaccharide A Obtained By Soaking within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl1376

b:48.6
occ:1.00
N A:ILE304 3.3 20.5 1.0
O A:HOH2201 3.4 43.5 1.0
CA A:HIS303 3.7 20.9 1.0
CB A:HIS303 3.9 21.5 1.0
CG1 A:ILE304 4.0 22.1 1.0
C A:HIS303 4.0 20.7 1.0
CB A:ILE304 4.1 21.0 1.0
CD1 A:ILE304 4.2 23.5 1.0
NZ A:LYS307 4.2 30.4 1.0
CA A:ILE304 4.3 19.8 1.0
CE A:LYS307 4.5 28.6 1.0
O A:HOH2365 4.7 36.4 1.0
N A:HIS303 4.9 20.8 1.0
O A:ALA302 4.9 20.7 1.0

Reference:

M.Lafond, G.Sulzenbacher, T.Freyd, B.Henrissat, J.G.Berrin, M.L.Garron. The Quaternary Structure of A Glycoside Hydrolase Dictates Specificity Towards Beta-Glucans J.Biol.Chem. V. 291 7183 2016.
ISSN: ISSN 0021-9258
PubMed: 26755730
DOI: 10.1074/JBC.M115.695999
Page generated: Sat Dec 12 11:29:51 2020

Last articles

Zn in 8WB0
Zn in 8WAX
Zn in 8WAU
Zn in 8WAZ
Zn in 8WAY
Zn in 8WAV
Zn in 8WAW
Zn in 8WAT
Zn in 8W7M
Zn in 8WD3
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy