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Chlorine in PDB 5amc: Crystal Structure of the Angiotensin-1 Converting Enzyme N- Domain in Complex with Amyloid-Beta Fluorogenic Fragment 4-10

Enzymatic activity of Crystal Structure of the Angiotensin-1 Converting Enzyme N- Domain in Complex with Amyloid-Beta Fluorogenic Fragment 4-10

All present enzymatic activity of Crystal Structure of the Angiotensin-1 Converting Enzyme N- Domain in Complex with Amyloid-Beta Fluorogenic Fragment 4-10:
3.4.15.1;

Protein crystallography data

The structure of Crystal Structure of the Angiotensin-1 Converting Enzyme N- Domain in Complex with Amyloid-Beta Fluorogenic Fragment 4-10, PDB code: 5amc was solved by G.Masuyer, K.M.Larmuth, R.G.Douglas, E.D.Sturrock, K.R.Acharya, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 74.53 / 1.65
Space group P 1
Cell size a, b, c (Å), α, β, γ (°) 73.029, 76.470, 83.158, 88.78, 64.25, 75.64
R / Rfree (%) 20.772 / 24.169

Other elements in 5amc:

The structure of Crystal Structure of the Angiotensin-1 Converting Enzyme N- Domain in Complex with Amyloid-Beta Fluorogenic Fragment 4-10 also contains other interesting chemical elements:

Zinc (Zn) 2 atoms

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Crystal Structure of the Angiotensin-1 Converting Enzyme N- Domain in Complex with Amyloid-Beta Fluorogenic Fragment 4-10 (pdb code 5amc). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of the Angiotensin-1 Converting Enzyme N- Domain in Complex with Amyloid-Beta Fluorogenic Fragment 4-10, PDB code: 5amc:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in 5amc

Go back to Chlorine Binding Sites List in 5amc
Chlorine binding site 1 out of 2 in the Crystal Structure of the Angiotensin-1 Converting Enzyme N- Domain in Complex with Amyloid-Beta Fluorogenic Fragment 4-10


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of the Angiotensin-1 Converting Enzyme N- Domain in Complex with Amyloid-Beta Fluorogenic Fragment 4-10 within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl1611

b:19.7
occ:1.00
OH A:TYR202 3.0 18.6 1.0
O A:HOH2201 3.1 18.9 1.0
NE A:ARG500 3.2 18.3 1.0
NH2 A:ARG500 3.4 18.0 1.0
CB A:ARG500 3.6 15.9 1.0
CB A:PRO385 3.7 18.8 1.0
CB A:PRO497 3.7 18.1 1.0
CE2 A:TYR202 3.7 20.6 1.0
N A:ARG500 3.8 15.8 1.0
CG A:PRO385 3.8 19.2 1.0
CZ A:ARG500 3.8 19.3 1.0
CZ A:TYR202 3.8 19.4 1.0
CZ3 A:TRP201 3.8 30.1 1.0
CE3 A:TRP201 3.9 29.7 1.0
CG2 A:ILE499 3.9 16.9 1.0
CA A:ARG500 4.1 15.8 1.0
CG A:ARG500 4.2 16.6 1.0
CD A:ARG500 4.3 18.0 1.0
CG A:PRO497 4.5 18.2 1.0
N A:ILE499 4.7 17.3 1.0
C A:ILE499 4.8 16.5 1.0
CD A:PRO385 4.8 19.6 1.0
C A:PRO497 4.8 18.1 1.0
O A:HOH2381 4.8 19.1 1.0
CA A:PRO497 4.9 18.2 1.0

Chlorine binding site 2 out of 2 in 5amc

Go back to Chlorine Binding Sites List in 5amc
Chlorine binding site 2 out of 2 in the Crystal Structure of the Angiotensin-1 Converting Enzyme N- Domain in Complex with Amyloid-Beta Fluorogenic Fragment 4-10


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of the Angiotensin-1 Converting Enzyme N- Domain in Complex with Amyloid-Beta Fluorogenic Fragment 4-10 within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cl1612

b:23.2
occ:1.00
O B:HOH2152 3.0 29.4 1.0
OH B:TYR202 3.1 24.2 1.0
NE B:ARG500 3.2 21.6 1.0
NH2 B:ARG500 3.5 21.8 1.0
CB B:ARG500 3.6 20.0 1.0
CB B:PRO497 3.7 20.5 1.0
N B:ARG500 3.7 18.1 1.0
CE1 B:TYR202 3.7 26.5 1.0
CB B:PRO385 3.8 24.8 1.0
CZ B:TYR202 3.8 25.9 1.0
CZ B:ARG500 3.8 21.7 1.0
CG2 B:ILE499 3.9 19.4 1.0
CZ3 B:TRP201 3.9 36.8 1.0
CE3 B:TRP201 3.9 37.3 1.0
CG B:PRO385 4.0 25.2 1.0
CA B:ARG500 4.1 18.8 1.0
CG B:ARG500 4.1 21.1 1.0
CD B:ARG500 4.3 21.3 1.0
CG B:PRO497 4.5 21.1 1.0
N B:ILE499 4.7 17.9 1.0
C B:ILE499 4.7 18.0 1.0
CD B:PRO385 4.7 25.4 1.0
O B:HOH2303 4.8 23.3 1.0
C B:PRO497 4.8 19.6 1.0
CA B:PRO497 4.9 20.3 1.0
N B:TYR498 5.0 18.0 1.0

Reference:

K.M.Larmuth, G.Masuyer, R.G.Douglas, E.D.Sturrock, K.R.Acharya. The Kinetic and Structural Characterisation of Amyloid-Beta Metabolism By Human Angiotensin-1- Converting Enzyme (Ace) Febs J. V. 283 1060 2016.
ISSN: ISSN 1742-464X
PubMed: 26748546
DOI: 10.1111/FEBS.13647
Page generated: Fri Jul 26 05:19:56 2024

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