Atomistry » Chlorine » PDB 5b1g-5bor » 5b2p
Atomistry »
  Chlorine »
    PDB 5b1g-5bor »
      5b2p »

Chlorine in PDB 5b2p: Crystal Structure of Francisella Novicida CAS9 in Complex with Sgrna and Target Dna (Tga Pam)

Protein crystallography data

The structure of Crystal Structure of Francisella Novicida CAS9 in Complex with Sgrna and Target Dna (Tga Pam), PDB code: 5b2p was solved by H.Hirano, H.Nishimasu, T.Nakane, R.Ishitani, O.Nureki, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 46.26 / 1.70
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 81.611, 159.273, 96.674, 90.00, 106.86, 90.00
R / Rfree (%) 18 / 20.3

Other elements in 5b2p:

The structure of Crystal Structure of Francisella Novicida CAS9 in Complex with Sgrna and Target Dna (Tga Pam) also contains other interesting chemical elements:

Zinc (Zn) 1 atom
Calcium (Ca) 17 atoms
Sodium (Na) 4 atoms

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Crystal Structure of Francisella Novicida CAS9 in Complex with Sgrna and Target Dna (Tga Pam) (pdb code 5b2p). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of Francisella Novicida CAS9 in Complex with Sgrna and Target Dna (Tga Pam), PDB code: 5b2p:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in 5b2p

Go back to Chlorine Binding Sites List in 5b2p
Chlorine binding site 1 out of 2 in the Crystal Structure of Francisella Novicida CAS9 in Complex with Sgrna and Target Dna (Tga Pam)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of Francisella Novicida CAS9 in Complex with Sgrna and Target Dna (Tga Pam) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl1704

b:43.5
occ:1.00
O A:HOH2268 2.8 44.1 1.0
N A:PHE602 3.1 34.0 1.0
O A:HOH2415 3.2 51.6 1.0
NZ A:LYS564 3.2 39.3 1.0
CE A:LYS564 3.6 47.6 1.0
CD2 A:HIS609 3.7 35.7 1.0
CD2 A:PHE602 3.8 35.2 1.0
CB A:PHE602 3.8 33.4 1.0
CA A:ILE601 3.9 35.5 1.0
CA A:PHE602 3.9 35.4 1.0
C A:ILE601 4.0 36.7 1.0
O A:PHE602 4.1 36.9 1.0
O A:GLY600 4.2 35.2 1.0
CB A:CYS612 4.2 32.9 1.0
CG A:PHE602 4.3 33.2 1.0
CA A:HIS609 4.3 29.4 1.0
CG A:HIS609 4.3 32.6 1.0
CG2 A:ILE601 4.4 36.8 1.0
C A:PHE602 4.4 36.8 1.0
SG A:CYS612 4.5 34.9 1.0
NE2 A:HIS609 4.5 35.7 1.0
CB A:HIS609 4.6 31.5 1.0
CB A:ILE601 4.7 39.4 1.0
O A:HIS609 4.8 28.5 1.0
CE2 A:PHE602 4.8 34.4 1.0
N A:ILE601 4.9 36.9 1.0
C A:GLY600 5.0 34.9 1.0

Chlorine binding site 2 out of 2 in 5b2p

Go back to Chlorine Binding Sites List in 5b2p
Chlorine binding site 2 out of 2 in the Crystal Structure of Francisella Novicida CAS9 in Complex with Sgrna and Target Dna (Tga Pam)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of Francisella Novicida CAS9 in Complex with Sgrna and Target Dna (Tga Pam) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl1705

b:29.0
occ:1.00
O A:HOH2015 3.1 32.5 1.0
NZ A:LYS370 3.2 32.0 1.0
CG A:LYS366 3.5 27.9 1.0
CG A:GLU399 3.6 30.3 1.0
CD A:LYS366 3.6 29.9 1.0
CD1 A:TRP395 3.6 25.8 1.0
CD A:LYS370 3.7 30.9 1.0
CB A:PRO367 3.7 29.0 1.0
CB A:TRP395 3.7 28.3 1.0
CD2 A:HIS394 3.8 30.9 1.0
CA A:PRO367 3.8 26.2 1.0
CE A:LYS370 3.9 33.4 1.0
N A:PRO367 3.9 24.6 1.0
CD A:PRO367 3.9 24.5 1.0
CA A:TRP395 3.9 28.5 1.0
CG A:TRP395 4.1 26.4 1.0
C A:LYS366 4.3 25.6 1.0
CD A:GLU399 4.3 34.6 1.0
CG A:PRO367 4.3 27.3 1.0
N A:TRP395 4.3 28.4 1.0
OE2 A:GLU399 4.4 32.0 1.0
NE2 A:HIS394 4.4 31.9 1.0
CB A:GLU399 4.4 28.4 1.0
CB A:LYS366 4.5 25.6 1.0
O A:LYS366 4.6 25.2 1.0
O A:THR391 4.8 30.5 1.0
NE1 A:TRP395 4.8 26.8 1.0
CG A:HIS394 4.9 33.1 1.0
C A:HIS394 5.0 31.2 1.0
CG A:LYS370 5.0 30.1 1.0

Reference:

H.Hirano, J.S.Gootenberg, T.Horii, O.O.Abudayyeh, M.Kimura, P.D.Hsu, T.Nakane, R.Ishitani, I.Hatada, F.Zhang, H.Nishimasu, O.Nureki. Structure and Engineering of Francisella Novicida CAS9 Cell V. 164 950 2016.
ISSN: ISSN 1097-4172
PubMed: 26875867
DOI: 10.1016/J.CELL.2016.01.039
Page generated: Sat Dec 12 11:31:50 2020

Last articles

Zn in 8WB0
Zn in 8WAX
Zn in 8WAU
Zn in 8WAZ
Zn in 8WAY
Zn in 8WAV
Zn in 8WAW
Zn in 8WAT
Zn in 8W7M
Zn in 8WD3
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy