Chlorine in PDB 5bxl: Yeast 20S Proteasome BETA2-G170A Mutant

All present enzymatic activity of Yeast 20S Proteasome BETA2-G170A Mutant:
3.4.25.1;

The structure of Yeast 20S Proteasome BETA2-G170A Mutant, PDB code: 5bxl was solved by E.M.Huber, M.Groll, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	15.00 / 2.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	134.800, 300.940, 144.490, 90.00, 112.71, 90.00
R / Rfree (%)	18.9 / 21.4

The structure of Yeast 20S Proteasome BETA2-G170A Mutant also contains other interesting chemical elements:

Magnesium

(Mg)

9 atoms

The binding sites of Chlorine atom in the Yeast 20S Proteasome BETA2-G170A Mutant (pdb code 5bxl). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Yeast 20S Proteasome BETA2-G170A Mutant, PDB code: 5bxl:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Yeast 20S Proteasome BETA2-G170A Mutant


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Yeast 20S Proteasome BETA2-G170A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ G:Cl302 b:38.2 occ:1.00 OH H:TYR69 2.9 39.6 1.0 NH1 G:ARG111 3.0 44.4 1.0 OD1 G:ASN114 3.1 44.8 1.0 CD G:ARG111 3.6 42.5 1.0 CB G:ASN114 3.6 39.4 1.0 CG G:ASN114 3.8 40.8 1.0 CZ H:TYR69 3.8 37.8 1.0 CE2 H:TYR69 3.8 36.8 1.0 CZ G:ARG111 4.0 43.8 1.0 NE G:ARG111 4.2 42.9 1.0 CA G:ARG111 4.6 41.3 1.0 O G:ARG111 4.7 44.4 1.0 CG G:ARG111 4.9 41.6 1.0

Chlorine binding site 2 out of 2 in the Yeast 20S Proteasome BETA2-G170A Mutant


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Yeast 20S Proteasome BETA2-G170A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ U:Cl301 b:41.8 occ:1.00 OH V:TYR69 2.9 45.2 1.0 OD1 U:ASN114 3.1 49.8 1.0 NH1 U:ARG111 3.1 48.2 1.0 CB U:ASN114 3.6 43.8 1.0 CG U:ASN114 3.7 45.9 1.0 CZ V:TYR69 3.7 45.9 1.0 CE2 V:TYR69 3.7 47.3 1.0 CD U:ARG111 3.8 46.4 1.0 CZ U:ARG111 4.2 48.1 1.0 NE U:ARG111 4.4 48.1 1.0 CA U:ARG111 4.7 43.9 1.0 O U:HOH421 4.7 42.5 1.0 O U:ARG111 4.8 44.2 1.0 ND2 U:ASN114 4.9 45.8 1.0 OD2 O:ASP87 5.0 58.9 1.0 CE1 V:TYR69 5.0 46.0 1.0

I.Treise, E.M.Huber, T.Klein-Rodewald, W.Heinemeyer, S.A.Grassmann, M.Basler, T.Adler, B.Rathkolb, L.Helming, C.Andres, M.Klaften, C.Landbrecht, T.Wieland, T.M.Strom, K.D.Mccoy, A.J.Macpherson, E.Wolf, M.Groettrup, M.Ollert, F.Neff, V.Gailus-Durner, H.Fuchs, M.Hrabe De Angelis, M.Groll, D.H.Busch. Defective Immuno- and Thymoproteasome Assembly Causes Severe Immunodeficiency. Sci Rep V. 8 5975 2018.
ISSN: ESSN 2045-2322
PubMed: 29654304
DOI: 10.1038/S41598-018-24199-0