Chlorine in PDB 5c80: X-Ray Structure Uridine Phosphorylase From Vibrio Cholerae in Complex with Uridine at 2.24 A Resolution
Enzymatic activity of X-Ray Structure Uridine Phosphorylase From Vibrio Cholerae in Complex with Uridine at 2.24 A Resolution
All present enzymatic activity of X-Ray Structure Uridine Phosphorylase From Vibrio Cholerae in Complex with Uridine at 2.24 A Resolution:
2.4.2.3;
Protein crystallography data
The structure of X-Ray Structure Uridine Phosphorylase From Vibrio Cholerae in Complex with Uridine at 2.24 A Resolution, PDB code: 5c80
was solved by
I.I.Prokofev,
A.A.Lashkov,
A.G.Gabdoulkhakov,
C.Betzel,
A.M.Mikhailov,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
45.47 /
2.24
|
Space group
|
P 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
61.255,
73.110,
83.137,
71.81,
77.19,
85.69
|
R / Rfree (%)
|
16.8 /
23.7
|
Other elements in 5c80:
The structure of X-Ray Structure Uridine Phosphorylase From Vibrio Cholerae in Complex with Uridine at 2.24 A Resolution also contains other interesting chemical elements:
Chlorine Binding Sites:
The binding sites of Chlorine atom in the X-Ray Structure Uridine Phosphorylase From Vibrio Cholerae in Complex with Uridine at 2.24 A Resolution
(pdb code 5c80). This binding sites where shown within
5.0 Angstroms radius around Chlorine atom.
In total 6 binding sites of Chlorine where determined in the
X-Ray Structure Uridine Phosphorylase From Vibrio Cholerae in Complex with Uridine at 2.24 A Resolution, PDB code: 5c80:
Jump to Chlorine binding site number:
1;
2;
3;
4;
5;
6;
Chlorine binding site 1 out
of 6 in 5c80
Go back to
Chlorine Binding Sites List in 5c80
Chlorine binding site 1 out
of 6 in the X-Ray Structure Uridine Phosphorylase From Vibrio Cholerae in Complex with Uridine at 2.24 A Resolution
Mono view
Stereo pair view
|
A full contact list of Chlorine with other atoms in the Cl binding
site number 1 of X-Ray Structure Uridine Phosphorylase From Vibrio Cholerae in Complex with Uridine at 2.24 A Resolution within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Cl304
b:41.8
occ:1.00
|
NH1
|
A:ARG29
|
3.0
|
64.2
|
1.0
|
O
|
A:HOH467
|
3.1
|
29.6
|
1.0
|
N
|
A:GLY25
|
3.2
|
25.3
|
1.0
|
N
|
A:ASP26
|
3.4
|
28.4
|
1.0
|
CA
|
A:GLY25
|
3.5
|
25.3
|
1.0
|
NH2
|
B:ARG47
|
3.6
|
27.0
|
1.0
|
CA
|
A:GLY92
|
3.9
|
25.2
|
1.0
|
C
|
A:GLY25
|
3.9
|
25.9
|
1.0
|
NH1
|
B:ARG47
|
4.0
|
27.1
|
1.0
|
CB
|
A:ASP26
|
4.0
|
29.4
|
1.0
|
O
|
A:VAL91
|
4.2
|
27.6
|
1.0
|
NH2
|
A:ARG90
|
4.2
|
24.2
|
0.5
|
CZ
|
A:ARG29
|
4.2
|
59.6
|
1.0
|
N
|
A:THR93
|
4.2
|
26.9
|
1.0
|
CZ
|
B:ARG47
|
4.3
|
26.4
|
1.0
|
CA
|
A:ASP26
|
4.4
|
29.1
|
1.0
|
C
|
A:GLY92
|
4.4
|
25.0
|
1.0
|
CG2
|
A:THR93
|
4.4
|
30.0
|
1.0
|
C
|
A:PRO24
|
4.4
|
26.0
|
1.0
|
CD
|
A:ARG29
|
4.6
|
49.5
|
1.0
|
OG1
|
A:THR93
|
4.8
|
28.9
|
1.0
|
CB
|
A:PRO24
|
4.8
|
26.2
|
1.0
|
NE
|
A:ARG29
|
4.8
|
56.9
|
1.0
|
CA
|
A:PRO24
|
4.9
|
25.9
|
1.0
|
N
|
A:GLY92
|
4.9
|
26.0
|
1.0
|
C
|
A:VAL91
|
5.0
|
25.6
|
1.0
|
NH2
|
A:ARG90
|
5.0
|
20.9
|
0.5
|
|
Chlorine binding site 2 out
of 6 in 5c80
Go back to
Chlorine Binding Sites List in 5c80
Chlorine binding site 2 out
of 6 in the X-Ray Structure Uridine Phosphorylase From Vibrio Cholerae in Complex with Uridine at 2.24 A Resolution
Mono view
Stereo pair view
|
A full contact list of Chlorine with other atoms in the Cl binding
site number 2 of X-Ray Structure Uridine Phosphorylase From Vibrio Cholerae in Complex with Uridine at 2.24 A Resolution within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Cl305
b:53.9
occ:1.00
|
N
|
A:TYR168
|
3.3
|
22.7
|
1.0
|
CA
|
A:ARG167
|
3.9
|
22.2
|
1.0
|
O
|
A:HOH471
|
3.9
|
55.1
|
1.0
|
C
|
A:ARG167
|
4.1
|
22.7
|
1.0
|
N
|
A:ASP169
|
4.1
|
27.2
|
1.0
|
CA
|
A:TYR168
|
4.2
|
23.4
|
1.0
|
O
|
A:GLU166
|
4.2
|
23.4
|
1.0
|
CB
|
A:TYR168
|
4.2
|
23.1
|
1.0
|
NH2
|
A:ARG222
|
4.2
|
26.4
|
1.0
|
CD2
|
A:TYR168
|
4.4
|
22.1
|
1.0
|
CB
|
A:ARG167
|
4.6
|
22.8
|
1.0
|
C
|
A:TYR168
|
4.6
|
25.4
|
1.0
|
CG
|
A:TYR168
|
4.8
|
22.8
|
1.0
|
CG
|
A:ARG167
|
4.8
|
22.2
|
1.0
|
N
|
A:ARG167
|
4.9
|
23.4
|
1.0
|
CB
|
A:ASP169
|
5.0
|
35.9
|
1.0
|
C
|
A:GLU166
|
5.0
|
21.8
|
1.0
|
|
Chlorine binding site 3 out
of 6 in 5c80
Go back to
Chlorine Binding Sites List in 5c80
Chlorine binding site 3 out
of 6 in the X-Ray Structure Uridine Phosphorylase From Vibrio Cholerae in Complex with Uridine at 2.24 A Resolution
Mono view
Stereo pair view
|
A full contact list of Chlorine with other atoms in the Cl binding
site number 3 of X-Ray Structure Uridine Phosphorylase From Vibrio Cholerae in Complex with Uridine at 2.24 A Resolution within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Cl305
b:45.7
occ:1.00
|
O
|
B:HOH480
|
3.2
|
39.4
|
1.0
|
N
|
B:GLY25
|
3.3
|
24.0
|
1.0
|
NH2
|
A:ARG47
|
3.5
|
36.6
|
1.0
|
N
|
B:ASP26
|
3.6
|
29.8
|
1.0
|
CA
|
B:GLY25
|
3.6
|
26.0
|
1.0
|
NH1
|
B:ARG29
|
3.8
|
43.6
|
1.0
|
CA
|
B:GLY92
|
3.9
|
20.6
|
1.0
|
NH1
|
A:ARG47
|
4.0
|
34.0
|
1.0
|
CB
|
B:ASP26
|
4.0
|
31.4
|
1.0
|
C
|
B:GLY25
|
4.1
|
28.1
|
1.0
|
O
|
B:VAL91
|
4.1
|
22.0
|
1.0
|
CZ
|
B:ARG29
|
4.1
|
47.1
|
1.0
|
CD
|
B:ARG29
|
4.2
|
39.3
|
1.0
|
CZ
|
A:ARG47
|
4.2
|
33.4
|
1.0
|
NE
|
B:ARG29
|
4.3
|
44.6
|
1.0
|
C
|
B:PRO24
|
4.4
|
23.4
|
1.0
|
N
|
B:THR93
|
4.4
|
23.4
|
1.0
|
CA
|
B:ASP26
|
4.5
|
31.6
|
1.0
|
C
|
B:GLY92
|
4.6
|
21.6
|
1.0
|
CA
|
B:PRO24
|
4.8
|
24.2
|
1.0
|
CB
|
B:PRO24
|
4.8
|
23.9
|
1.0
|
NH2
|
B:ARG90
|
4.8
|
33.3
|
1.0
|
N
|
B:GLY92
|
4.8
|
20.2
|
1.0
|
C
|
B:VAL91
|
4.9
|
20.7
|
1.0
|
NH2
|
B:ARG29
|
4.9
|
46.7
|
1.0
|
OG1
|
B:THR93
|
4.9
|
27.7
|
1.0
|
|
Chlorine binding site 4 out
of 6 in 5c80
Go back to
Chlorine Binding Sites List in 5c80
Chlorine binding site 4 out
of 6 in the X-Ray Structure Uridine Phosphorylase From Vibrio Cholerae in Complex with Uridine at 2.24 A Resolution
Mono view
Stereo pair view
|
A full contact list of Chlorine with other atoms in the Cl binding
site number 4 of X-Ray Structure Uridine Phosphorylase From Vibrio Cholerae in Complex with Uridine at 2.24 A Resolution within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Cl304
b:56.8
occ:1.00
|
N
|
C:GLY25
|
3.1
|
25.2
|
1.0
|
O
|
C:HOH420
|
3.2
|
37.6
|
1.0
|
NH2
|
C:ARG29
|
3.4
|
61.0
|
1.0
|
N
|
C:ASP26
|
3.7
|
32.4
|
1.0
|
CZ
|
C:ARG29
|
3.7
|
55.9
|
1.0
|
O
|
C:VAL91
|
3.7
|
26.2
|
1.0
|
CA
|
C:GLY25
|
3.7
|
28.1
|
1.0
|
NE
|
C:ARG29
|
4.0
|
50.2
|
1.0
|
CD
|
C:ARG29
|
4.0
|
47.3
|
1.0
|
C
|
C:PRO24
|
4.1
|
25.8
|
1.0
|
CB
|
C:PRO24
|
4.1
|
23.6
|
1.0
|
CA
|
C:GLY92
|
4.1
|
25.6
|
1.0
|
O
|
C:HOH430
|
4.1
|
31.0
|
1.0
|
CA
|
C:PRO24
|
4.2
|
24.0
|
1.0
|
C
|
C:GLY25
|
4.3
|
30.5
|
1.0
|
NH1
|
C:ARG29
|
4.4
|
51.5
|
1.0
|
CB
|
C:ASP26
|
4.4
|
33.9
|
1.0
|
C
|
C:VAL91
|
4.6
|
26.9
|
1.0
|
NH2
|
C:ARG90
|
4.7
|
28.7
|
1.0
|
CA
|
C:ASP26
|
4.7
|
34.5
|
1.0
|
N
|
C:THR93
|
4.7
|
26.5
|
1.0
|
NH1
|
D:ARG47
|
4.7
|
30.2
|
1.0
|
C
|
C:GLY92
|
4.8
|
26.4
|
1.0
|
N
|
C:GLY92
|
4.9
|
25.6
|
1.0
|
|
Chlorine binding site 5 out
of 6 in 5c80
Go back to
Chlorine Binding Sites List in 5c80
Chlorine binding site 5 out
of 6 in the X-Ray Structure Uridine Phosphorylase From Vibrio Cholerae in Complex with Uridine at 2.24 A Resolution
Mono view
Stereo pair view
|
A full contact list of Chlorine with other atoms in the Cl binding
site number 5 of X-Ray Structure Uridine Phosphorylase From Vibrio Cholerae in Complex with Uridine at 2.24 A Resolution within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Cl305
b:55.3
occ:1.00
|
N
|
D:GLY25
|
3.0
|
21.9
|
1.0
|
O
|
D:HOH435
|
3.2
|
48.5
|
1.0
|
N
|
D:ASP26
|
3.2
|
28.7
|
1.0
|
CA
|
D:GLY25
|
3.4
|
25.2
|
1.0
|
NH2
|
C:ARG47
|
3.7
|
40.7
|
1.0
|
C
|
D:GLY25
|
3.8
|
27.3
|
1.0
|
NE
|
D:ARG29
|
4.0
|
51.5
|
1.0
|
C
|
D:PRO24
|
4.1
|
21.2
|
1.0
|
O
|
D:VAL91
|
4.1
|
23.9
|
1.0
|
CZ
|
D:ARG29
|
4.1
|
55.0
|
1.0
|
CB
|
D:ASP26
|
4.1
|
28.6
|
1.0
|
CD
|
D:ARG29
|
4.2
|
45.7
|
1.0
|
NH1
|
D:ARG29
|
4.3
|
51.7
|
1.0
|
CA
|
D:ASP26
|
4.3
|
29.4
|
1.0
|
CA
|
D:GLY92
|
4.3
|
28.3
|
1.0
|
CB
|
D:PRO24
|
4.4
|
20.4
|
1.0
|
CA
|
D:PRO24
|
4.4
|
21.6
|
1.0
|
NH1
|
C:ARG47
|
4.4
|
37.5
|
1.0
|
CZ
|
C:ARG47
|
4.5
|
36.4
|
1.0
|
NH2
|
D:ARG29
|
4.6
|
52.0
|
1.0
|
NH2
|
D:ARG90
|
4.7
|
34.7
|
1.0
|
C
|
D:VAL91
|
5.0
|
25.4
|
1.0
|
N
|
D:THR93
|
5.0
|
30.4
|
1.0
|
|
Chlorine binding site 6 out
of 6 in 5c80
Go back to
Chlorine Binding Sites List in 5c80
Chlorine binding site 6 out
of 6 in the X-Ray Structure Uridine Phosphorylase From Vibrio Cholerae in Complex with Uridine at 2.24 A Resolution
Mono view
Stereo pair view
|
A full contact list of Chlorine with other atoms in the Cl binding
site number 6 of X-Ray Structure Uridine Phosphorylase From Vibrio Cholerae in Complex with Uridine at 2.24 A Resolution within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
F:Cl306
b:44.5
occ:1.00
|
N
|
F:GLY25
|
3.2
|
21.9
|
1.0
|
O
|
F:HOH488
|
3.4
|
31.1
|
1.0
|
N
|
F:ASP26
|
3.4
|
28.4
|
1.0
|
NE
|
F:ARG29
|
3.6
|
42.9
|
1.0
|
CA
|
F:GLY25
|
3.7
|
24.5
|
1.0
|
CB
|
F:ASP26
|
3.9
|
29.2
|
1.0
|
O
|
F:VAL91
|
3.9
|
24.4
|
1.0
|
CZ
|
F:ARG29
|
4.0
|
45.0
|
1.0
|
NH2
|
E:ARG47
|
4.0
|
42.1
|
1.0
|
C
|
F:GLY25
|
4.0
|
26.0
|
1.0
|
CD
|
F:ARG29
|
4.0
|
37.9
|
1.0
|
CA
|
F:GLY92
|
4.0
|
23.1
|
1.0
|
NH2
|
F:ARG29
|
4.2
|
44.4
|
1.0
|
C
|
F:PRO24
|
4.2
|
22.4
|
1.0
|
CA
|
F:ASP26
|
4.3
|
28.9
|
1.0
|
NH1
|
E:ARG47
|
4.5
|
39.4
|
1.0
|
CB
|
F:PRO24
|
4.5
|
21.4
|
1.0
|
CA
|
F:PRO24
|
4.5
|
21.1
|
1.0
|
N
|
F:THR93
|
4.6
|
24.4
|
1.0
|
NH2
|
F:ARG90
|
4.6
|
15.6
|
0.5
|
CZ
|
E:ARG47
|
4.7
|
39.1
|
1.0
|
NH1
|
F:ARG29
|
4.7
|
43.2
|
1.0
|
C
|
F:GLY92
|
4.7
|
23.9
|
1.0
|
CG2
|
F:THR93
|
4.8
|
26.5
|
1.0
|
C
|
F:VAL91
|
4.8
|
22.8
|
1.0
|
N
|
F:GLY92
|
5.0
|
22.0
|
1.0
|
NH2
|
F:ARG90
|
5.0
|
17.8
|
0.5
|
|
Reference:
I.I.Prokofev,
A.A.Lashkov,
A.G.Gabdoulkhakov,
V.V.Balaev,
T.A.Seregina,
A.S.Mironov,
C.Betzel,
A.M.Mikhailov.
X-Ray Structures of Uridine Phosphorylase From Vibrio Cholerae in Complexes with Uridine, Thymidine, Uracil, Thymine, and Phosphate Anion: Substrate Specificity of Bacterial Uridine Phosphorylases Crystallography Reports V. 61 954 2016.
ISSN: ISSN 1063-7745
DOI: 10.1134/S1063774516060134
Page generated: Fri Jul 26 05:58:47 2024
|