Chlorine in PDB 5cza: Yeast 20S Proteasome BETA5-D166N Mutant

All present enzymatic activity of Yeast 20S Proteasome BETA5-D166N Mutant:
3.4.25.1;

The structure of Yeast 20S Proteasome BETA5-D166N Mutant, PDB code: 5cza was solved by E.M.Huber, M.Groll, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	15.00 / 2.50
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	134.770, 301.430, 144.140, 90.00, 112.84, 90.00
R / Rfree (%)	20.6 / 22.4

The structure of Yeast 20S Proteasome BETA5-D166N Mutant also contains other interesting chemical elements:

Magnesium

(Mg)

8 atoms

The binding sites of Chlorine atom in the Yeast 20S Proteasome BETA5-D166N Mutant (pdb code 5cza). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Yeast 20S Proteasome BETA5-D166N Mutant, PDB code: 5cza:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Yeast 20S Proteasome BETA5-D166N Mutant


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Yeast 20S Proteasome BETA5-D166N Mutant within 5.0Å range: probe atom residue distance (Å) B Occ G:Cl302 b:33.9 occ:1.00 NH1 G:ARG111 3.0 38.2 1.0 OH H:TYR69 3.0 33.6 1.0 OD1 G:ASN114 3.3 33.5 1.0 CD G:ARG111 3.6 35.0 1.0 CE2 H:TYR69 3.7 32.9 1.0 CB G:ASN114 3.8 31.2 1.0 CZ H:TYR69 3.8 32.7 1.0 CG G:ASN114 3.9 32.5 1.0 CZ G:ARG111 4.0 37.0 1.0 NE G:ARG111 4.2 36.6 1.0 CA G:ARG111 4.7 32.7 1.0 O G:ARG111 4.8 34.1 1.0 CG G:ARG111 4.9 33.8 1.0

Chlorine binding site 2 out of 2 in the Yeast 20S Proteasome BETA5-D166N Mutant


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Yeast 20S Proteasome BETA5-D166N Mutant within 5.0Å range: probe atom residue distance (Å) B Occ U:Cl301 b:37.7 occ:1.00 OH V:TYR69 3.0 41.5 1.0 NH1 U:ARG111 3.0 40.9 1.0 OD1 U:ASN114 3.2 40.6 1.0 CB U:ASN114 3.7 36.5 1.0 CD U:ARG111 3.7 39.4 1.0 CE2 V:TYR69 3.8 44.0 1.0 CZ V:TYR69 3.8 43.1 1.0 CG U:ASN114 3.8 38.0 1.0 CZ U:ARG111 4.1 40.4 1.0 NE U:ARG111 4.3 40.6 1.0 CA U:ARG111 4.8 37.2 1.0 O U:HOH451 4.8 52.0 1.0 O U:ARG111 4.9 36.5 1.0

E.M.Huber, W.Heinemeyer, X.Li, C.S.Arendt, M.Hochstrasser, M.Groll. A Unified Mechanism For Proteolysis and Autocatalytic Activation in the 20S Proteasome. Nat Commun V. 7 10900 2016.
ISSN: ESSN 2041-1723
PubMed: 26964885
DOI: 10.1038/NCOMMS10900