Chlorine in PDB 5dm9: Xfel Structure of Hen Egg-White Lysozyme Solved Using A Droplet Injector at Sacla

All present enzymatic activity of Xfel Structure of Hen Egg-White Lysozyme Solved Using A Droplet Injector at Sacla:
3.2.1.17;

The structure of Xfel Structure of Hen Egg-White Lysozyme Solved Using A Droplet Injector at Sacla, PDB code: 5dm9 was solved by J.Kobayashi, E.Nango, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.60 / 2.30
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	79.200, 79.200, 38.000, 90.00, 90.00, 90.00
R / Rfree (%)	18.2 / 21.2

The structure of Xfel Structure of Hen Egg-White Lysozyme Solved Using A Droplet Injector at Sacla also contains other interesting chemical elements:

Sodium

(Na)

1 atom

The binding sites of Chlorine atom in the Xfel Structure of Hen Egg-White Lysozyme Solved Using A Droplet Injector at Sacla (pdb code 5dm9). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Xfel Structure of Hen Egg-White Lysozyme Solved Using A Droplet Injector at Sacla, PDB code: 5dm9:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Xfel Structure of Hen Egg-White Lysozyme Solved Using A Droplet Injector at Sacla


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Xfel Structure of Hen Egg-White Lysozyme Solved Using A Droplet Injector at Sacla within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl201 b:30.4 occ:1.00 ND2 A:ASN113 3.2 29.6 1.0 CB A:ASN113 3.6 28.0 1.0 CG2 A:VAL109 3.9 29.5 1.0 O A:VAL109 3.9 25.3 1.0 CA A:ALA110 3.9 26.6 1.0 CG A:ASN113 3.9 29.7 1.0 C A:VAL109 4.2 27.3 1.0 N A:ALA110 4.2 26.2 1.0 CB A:ALA110 4.5 24.6 1.0 CD A:ARG114 4.5 26.2 1.0 CG A:ARG114 4.6 26.4 1.0 CB A:VAL109 4.8 29.0 1.0 CG1 A:VAL109 4.9 27.2 1.0 C A:ALA110 4.9 26.3 1.0 CA A:ASN113 4.9 27.9 1.0 O A:ALA110 5.0 25.6 1.0

Chlorine binding site 2 out of 2 in the Xfel Structure of Hen Egg-White Lysozyme Solved Using A Droplet Injector at Sacla


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Xfel Structure of Hen Egg-White Lysozyme Solved Using A Droplet Injector at Sacla within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl202 b:39.6 occ:1.00 O A:HOH348 3.5 34.6 1.0 N A:ASN59 3.8 28.3 1.0 O A:HOH356 3.9 39.1 1.0 O A:GLN57 4.0 25.9 1.0 CA A:ILE58 4.0 26.9 1.0 NE1 A:TRP63 4.2 30.2 1.0 CD1 A:TRP63 4.3 30.2 1.0 CD1 A:ILE98 4.3 28.0 1.0 CB A:ASN59 4.3 30.2 1.0 NE1 A:TRP108 4.4 25.1 1.0 O A:ALA107 4.4 28.2 1.0 C A:ILE58 4.5 28.7 1.0 CD1 A:TRP108 4.7 24.1 1.0 CE2 A:TRP108 4.7 25.0 1.0 CA A:ASN59 4.7 28.8 1.0 C A:GLN57 4.8 27.8 1.0 CG2 A:ILE98 4.8 27.5 1.0 O A:HOH338 4.8 29.1 1.0 CG2 A:ILE58 4.8 27.1 1.0 N A:ILE58 4.8 25.7 1.0 CB A:ILE58 4.8 27.0 1.0 CB A:ALA107 4.9 26.6 1.0 CG1 A:ILE58 5.0 26.2 1.0 C A:ALA107 5.0 26.6 1.0

F.Mafune, K.Miyajima, K.Tono, Y.Takeda, J.Y.Kohno, N.Miyauchi, J.Kobayashi, Y.Joti, E.Nango, S.Iwata, M.Yabashi. Microcrystal Delivery By Pulsed Liquid Droplet For Serial Femtosecond Crystallography. Acta Crystallogr D Struct V. 72 520 2016BIOL.
ISSN: ISSN 2059-7983
PubMed: 27050131
DOI: 10.1107/S2059798316001480