Chlorine in PDB 5f48: Crystal Structure of An Aminoglycoside Acetyltransferase Meta-AAC0020 From An Uncultured Soil Metagenomic Sample in Complex with Coenzyme A

The structure of Crystal Structure of An Aminoglycoside Acetyltransferase Meta-AAC0020 From An Uncultured Soil Metagenomic Sample in Complex with Coenzyme A, PDB code: 5f48 was solved by Z.Xu, T.Skarina, P.J.Stogios, V.Yim, A.Savchenko, W.F.Anderson, Center Forstructural Genomics Of Infectious Diseases (Csgid), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	23.62 / 1.95
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	46.133, 80.187, 53.926, 90.00, 91.97, 90.00
R / Rfree (%)	19 / 23.5

The structure of Crystal Structure of An Aminoglycoside Acetyltransferase Meta-AAC0020 From An Uncultured Soil Metagenomic Sample in Complex with Coenzyme A also contains other interesting chemical elements:

Magnesium

(Mg)

3 atoms

The binding sites of Chlorine atom in the Crystal Structure of An Aminoglycoside Acetyltransferase Meta-AAC0020 From An Uncultured Soil Metagenomic Sample in Complex with Coenzyme A (pdb code 5f48). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the Crystal Structure of An Aminoglycoside Acetyltransferase Meta-AAC0020 From An Uncultured Soil Metagenomic Sample in Complex with Coenzyme A, PDB code: 5f48:

Chlorine binding site 1 out of 1 in the Crystal Structure of An Aminoglycoside Acetyltransferase Meta-AAC0020 From An Uncultured Soil Metagenomic Sample in Complex with Coenzyme A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of An Aminoglycoside Acetyltransferase Meta-AAC0020 From An Uncultured Soil Metagenomic Sample in Complex with Coenzyme A within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl202 b:56.7 occ:1.00 NE A:ARG29 2.9 30.6 1.0 CG2 A:ILE25 3.4 26.0 1.0 NH2 A:ARG29 3.5 33.8 1.0 CZ A:ARG29 3.7 32.5 1.0 O A:HOH416 3.8 60.6 1.0 CG A:ARG29 3.8 27.8 1.0 O A:HOH456 3.9 33.9 1.0 CD A:ARG29 3.9 29.0 1.0 O A:HOH488 4.3 48.6 1.0 CB A:ILE25 4.8 23.9 1.0 CD1 A:ILE42 4.9 25.0 1.0 NH1 A:ARG29 5.0 32.8 1.0 O A:HOH468 5.0 47.1 1.0

Z.Xu, P.J.Stogios, A.T.Quaile, K.J.Forsberg, S.Patel, T.Skarina, S.Houliston, C.Arrowsmith, G.Dantas, A.Savchenko. Structural and Functional Survey of Environmental Aminoglycoside Acetyltransferases Reveals Functionality of Resistance Enzymes. Acs Infect Dis V. 3 653 2017.
ISSN: ESSN 2373-8227
PubMed: 28756664
DOI: 10.1021/ACSINFECDIS.7B00068