Chlorine in PDB 5f4q: Crystal Structure of the Human Egg Surface Protein Juno

The structure of Crystal Structure of the Human Egg Surface Protein Juno, PDB code: 5f4q was solved by H.Aydin, A.Sultana, J.E.Lee, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.68 / 1.80
Space group	P 41
Cell size a, b, c (Å), α, β, γ (°)	73.150, 73.150, 163.100, 90.00, 90.00, 90.00
R / Rfree (%)	19.6 / 22.7

The binding sites of Chlorine atom in the Crystal Structure of the Human Egg Surface Protein Juno (pdb code 5f4q). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the Crystal Structure of the Human Egg Surface Protein Juno, PDB code: 5f4q:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in the Crystal Structure of the Human Egg Surface Protein Juno


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of the Human Egg Surface Protein Juno within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl302 b:10.2 occ:1.00 HD22 A:ASN219 2.1 13.9 1.0 HG A:SER100 2.2 16.1 1.0 HD22 A:ASN102 2.4 14.5 1.0 HE1 A:TRP50 2.5 15.0 1.0 HB2 A:ASN219 2.8 14.6 1.0 HD21 A:ASN217 2.9 28.5 1.0 OG A:SER100 2.9 13.4 1.0 ND2 A:ASN219 2.9 11.6 1.0 HE1 A:PHE96 3.2 10.6 1.0 HB2 A:ASN102 3.2 17.1 1.0 ND2 A:ASN102 3.2 12.1 1.0 HB2 A:SER100 3.3 14.6 1.0 NE1 A:TRP50 3.3 12.5 1.0 HD21 A:ASN219 3.5 13.9 1.0 CB A:ASN219 3.5 12.2 1.0 HD2 A:PRO101 3.5 13.1 1.0 CB A:SER100 3.6 12.1 1.0 HB3 A:ASN219 3.6 14.6 1.0 HD21 A:ASN102 3.7 14.5 1.0 ND2 A:ASN217 3.7 23.7 1.0 CG A:ASN219 3.7 11.9 1.0 HE1 A:PHE211 3.7 16.9 1.0 HA A:SER100 3.7 14.7 1.0 HD1 A:TRP50 3.9 15.0 1.0 HD1 A:PHE96 3.9 10.1 1.0 CE1 A:PHE96 4.0 8.8 1.0 CD1 A:TRP50 4.0 12.5 1.0 OD1 A:ASN217 4.0 14.5 1.0 CB A:ASN102 4.0 14.2 1.0 CG A:ASN102 4.1 12.2 1.0 HD23 A:LEU207 4.2 14.2 1.0 CA A:SER100 4.2 12.2 1.0 H A:ASN102 4.2 16.3 1.0 CG A:ASN217 4.3 21.9 1.0 HD22 A:ASN217 4.3 28.5 1.0 CD1 A:PHE96 4.3 8.4 1.0 HB3 A:SER100 4.4 14.6 1.0 CE2 A:TRP50 4.4 11.7 1.0 CD A:PRO101 4.5 10.9 1.0 CE1 A:PHE211 4.5 14.0 1.0 HZ2 A:TRP50 4.5 13.3 1.0 HB3 A:ASN102 4.6 17.1 1.0 HZ A:PHE211 4.7 18.5 1.0 H A:ASN219 4.8 16.0 1.0 HD3 A:PRO101 4.8 13.1 1.0 N A:ASN102 4.8 13.6 1.0 CA A:ASN219 4.9 13.2 1.0 OD1 A:ASN219 4.9 11.7 1.0 CZ2 A:TRP50 4.9 11.1 1.0 N A:PRO101 5.0 12.2 1.0 C A:SER100 5.0 9.8 1.0

Chlorine binding site 2 out of 4 in the Crystal Structure of the Human Egg Surface Protein Juno


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of the Human Egg Surface Protein Juno within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl302 b:7.6 occ:1.00 HG B:SER100 2.3 11.7 1.0 HD22 B:ASN219 2.4 12.5 1.0 HD22 B:ASN102 2.4 12.2 1.0 HE1 B:TRP50 2.4 14.4 1.0 HD21 B:ASN217 2.8 25.3 1.0 HB2 B:ASN219 2.9 14.4 1.0 OG B:SER100 3.0 9.7 1.0 HB2 B:SER100 3.2 11.9 1.0 HB2 B:ASN102 3.2 11.7 1.0 HE1 B:PHE96 3.2 12.9 1.0 ND2 B:ASN102 3.2 10.2 1.0 NE1 B:TRP50 3.2 12.0 1.0 ND2 B:ASN219 3.2 10.4 1.0 HD2 B:PRO101 3.3 14.4 1.0 CB B:SER100 3.5 9.9 1.0 HD1 B:TRP50 3.6 15.4 1.0 ND2 B:ASN217 3.6 21.1 1.0 CB B:ASN219 3.6 12.0 1.0 HD21 B:ASN102 3.6 12.2 1.0 HA B:SER100 3.6 13.1 1.0 HB3 B:ASN219 3.7 14.4 1.0 CD1 B:TRP50 3.8 12.8 1.0 HD21 B:ASN219 3.8 12.5 1.0 HD1 B:PHE96 3.9 12.3 1.0 HE1 B:PHE211 3.9 18.5 1.0 CG B:ASN219 3.9 10.9 1.0 CE1 B:PHE96 4.0 10.7 1.0 CB B:ASN102 4.0 9.7 1.0 CG B:ASN102 4.0 10.3 1.0 H B:ASN102 4.0 13.1 1.0 OD1 B:ASN217 4.0 20.3 1.0 CA B:SER100 4.1 10.9 1.0 HD22 B:ASN217 4.2 25.3 1.0 CD B:PRO101 4.3 12.0 1.0 CG B:ASN217 4.3 20.7 1.0 HD23 B:LEU207 4.3 12.3 1.0 CD1 B:PHE96 4.3 10.3 1.0 HB3 B:SER100 4.4 11.9 1.0 CE2 B:TRP50 4.4 11.2 1.0 HD3 B:PRO101 4.6 14.4 1.0 HB3 B:ASN102 4.6 11.7 1.0 N B:ASN102 4.7 10.9 1.0 HZ2 B:TRP50 4.7 13.3 1.0 CE1 B:PHE211 4.7 15.4 1.0 N B:PRO101 4.7 12.0 1.0 C B:SER100 4.8 11.7 1.0 CA B:ASN102 4.9 10.3 1.0 H B:ASN219 4.9 17.1 1.0 CA B:ASN219 5.0 12.4 1.0

Chlorine binding site 3 out of 4 in the Crystal Structure of the Human Egg Surface Protein Juno


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Crystal Structure of the Human Egg Surface Protein Juno within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl302 b:10.9 occ:1.00 HE1 C:TRP50 2.3 15.2 1.0 HD22 C:ASN102 2.4 14.5 1.0 HD21 C:ASN217 2.5 17.2 1.0 HD22 C:ASN219 2.6 14.0 1.0 HG C:SER100 2.7 12.8 1.0 NE1 C:TRP50 3.0 12.7 1.0 HB2 C:ASN219 3.1 13.6 1.0 ND2 C:ASN102 3.3 12.1 1.0 HD2 C:PRO101 3.3 15.0 1.0 ND2 C:ASN217 3.3 14.3 1.0 HB2 C:SER100 3.3 12.8 1.0 HD1 C:TRP50 3.4 15.3 1.0 HB2 C:ASN102 3.4 14.5 1.0 OG C:SER100 3.4 10.7 1.0 ND2 C:ASN219 3.4 11.7 1.0 HE2 C:PHE211 3.5 17.0 1.0 CD1 C:TRP50 3.6 12.8 1.0 HA C:SER100 3.6 12.7 1.0 HD21 C:ASN102 3.7 14.5 1.0 CB C:SER100 3.8 10.7 1.0 OD1 C:ASN217 3.8 16.6 1.0 HE1 C:PHE96 3.8 12.0 1.0 CB C:ASN219 3.8 11.4 1.0 HD22 C:ASN217 3.9 17.2 1.0 HB3 C:ASN219 3.9 13.6 1.0 HD21 C:ASN219 4.0 14.0 1.0 CG C:ASN217 4.0 14.0 1.0 CG C:ASN219 4.1 11.4 1.0 HD1 C:PHE96 4.2 11.5 1.0 CB C:ASN102 4.2 12.1 1.0 CD C:PRO101 4.2 12.5 1.0 CA C:SER100 4.2 10.6 1.0 CG C:ASN102 4.2 12.4 1.0 HZ C:PHE211 4.3 17.7 1.0 CE2 C:PHE211 4.3 14.1 1.0 CE2 C:TRP50 4.3 12.2 1.0 O C:HOH555 4.3 17.0 1.0 H C:ASN102 4.4 14.4 1.0 HD23 C:LEU207 4.5 13.8 1.0 HD3 C:PRO101 4.5 15.0 1.0 CE1 C:PHE96 4.5 10.0 1.0 HZ2 C:TRP50 4.6 13.9 1.0 HB3 C:SER100 4.7 12.8 1.0 CZ C:PHE211 4.7 14.8 1.0 CD1 C:PHE96 4.8 9.6 1.0 HB3 C:ASN102 4.8 14.5 1.0 N C:PRO101 4.8 11.5 1.0 N C:ASN102 4.9 12.0 1.0 CZ2 C:TRP50 4.9 11.6 1.0 H C:ASN219 4.9 17.5 1.0 C C:SER100 4.9 11.9 1.0 CG C:TRP50 4.9 12.5 1.0 HG2 C:PRO101 5.0 14.8 1.0

Chlorine binding site 4 out of 4 in the Crystal Structure of the Human Egg Surface Protein Juno


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Crystal Structure of the Human Egg Surface Protein Juno within 5.0Å range: probe atom residue distance (Å) B Occ D:Cl302 b:8.9 occ:1.00 HE1 D:TRP50 2.3 11.9 1.0 HD22 D:ASN219 2.5 17.6 1.0 HD22 D:ASN102 2.5 15.1 1.0 HG D:SER100 2.6 13.7 1.0 HD21 D:ASN217 2.6 23.6 1.0 NE1 D:TRP50 3.0 9.9 1.0 HB2 D:ASN219 3.1 17.0 1.0 HD2 D:PRO101 3.2 14.5 1.0 HD1 D:TRP50 3.3 11.4 1.0 OG D:SER100 3.3 11.4 1.0 ND2 D:ASN219 3.3 14.7 1.0 ND2 D:ASN102 3.3 12.6 1.0 ND2 D:ASN217 3.4 19.6 1.0 HB2 D:SER100 3.4 13.3 1.0 HE1 D:PHE211 3.5 11.8 1.0 CD1 D:TRP50 3.5 9.5 1.0 HE1 D:PHE96 3.5 12.9 1.0 HA D:SER100 3.6 13.5 1.0 HB2 D:ASN102 3.6 15.3 1.0 HD21 D:ASN102 3.7 15.1 1.0 OD1 D:ASN217 3.7 18.3 1.0 CB D:SER100 3.8 11.1 1.0 CB D:ASN219 3.8 14.1 1.0 HD21 D:ASN219 3.9 17.6 1.0 HB3 D:ASN219 4.0 17.0 1.0 CG D:ASN217 4.0 19.8 1.0 HD22 D:ASN217 4.1 23.6 1.0 CG D:ASN219 4.1 14.1 1.0 HD1 D:PHE96 4.1 12.5 1.0 CD D:PRO101 4.2 12.1 1.0 CA D:SER100 4.2 11.2 1.0 CE1 D:PHE211 4.2 9.8 1.0 CE2 D:TRP50 4.3 10.2 1.0 HZ D:PHE211 4.3 11.7 1.0 H D:ASN102 4.3 15.2 1.0 CE1 D:PHE96 4.3 10.8 1.0 CG D:ASN102 4.3 12.9 1.0 CB D:ASN102 4.4 12.8 1.0 HD3 D:PRO101 4.4 14.5 1.0 O D:HOH545 4.5 12.6 1.0 CD1 D:PHE96 4.6 10.4 1.0 CZ D:PHE211 4.7 9.8 1.0 HZ2 D:TRP50 4.7 12.4 1.0 HD23 D:LEU207 4.7 10.4 1.0 HB3 D:SER100 4.7 13.3 1.0 N D:PRO101 4.8 12.2 1.0 CG D:TRP50 4.9 9.5 1.0 H D:ASN219 4.9 18.2 1.0 CZ2 D:TRP50 4.9 10.3 1.0 N D:ASN102 4.9 12.7 1.0 C D:SER100 4.9 11.8 1.0

H.Aydin, A.Sultana, S.Li, A.Thavalingam, J.E.Lee. Molecular Architecture of the Human Sperm IZUMO1 and Egg Juno Fertilization Complex. Nature V. 534 562 2016.
ISSN: ESSN 1476-4687
PubMed: 27309818
DOI: 10.1038/NATURE18595