Chlorine in PDB 5fg7: Yeast 20S Proteasome BETA2-T1A Mutant

All present enzymatic activity of Yeast 20S Proteasome BETA2-T1A Mutant:
3.4.25.1;

The structure of Yeast 20S Proteasome BETA2-T1A Mutant, PDB code: 5fg7 was solved by E.M.Huber, M.Groll, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	15.00 / 2.70
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	135.000, 301.730, 144.020, 90.00, 113.06, 90.00
R / Rfree (%)	20.6 / 23.1

The structure of Yeast 20S Proteasome BETA2-T1A Mutant also contains other interesting chemical elements:

Magnesium

(Mg)

8 atoms

The binding sites of Chlorine atom in the Yeast 20S Proteasome BETA2-T1A Mutant (pdb code 5fg7). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Yeast 20S Proteasome BETA2-T1A Mutant, PDB code: 5fg7:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Yeast 20S Proteasome BETA2-T1A Mutant


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Yeast 20S Proteasome BETA2-T1A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ G:Cl302 b:30.0 occ:1.00 OH H:TYR69 2.8 34.4 1.0 NH1 G:ARG111 3.0 42.6 1.0 ND2 G:ASN114 3.0 35.0 1.0 CD G:ARG111 3.5 38.1 1.0 CB G:ASN114 3.6 34.6 1.0 CZ H:TYR69 3.7 38.3 1.0 CG G:ASN114 3.8 35.3 1.0 CE2 H:TYR69 3.8 39.9 1.0 CZ G:ARG111 4.0 41.4 1.0 NE G:ARG111 4.2 40.0 1.0 CA G:ARG111 4.6 36.3 1.0 O G:ARG111 4.7 37.6 1.0 CG G:ARG111 4.8 37.3 1.0 OD1 G:ASN114 4.9 38.2 1.0 CE1 H:TYR69 5.0 39.1 1.0 CB G:ARG111 5.0 36.7 1.0

Chlorine binding site 2 out of 2 in the Yeast 20S Proteasome BETA2-T1A Mutant


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Yeast 20S Proteasome BETA2-T1A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ U:Cl301 b:30.0 occ:1.00 OH V:TYR69 2.5 46.6 1.0 ND2 U:ASN114 3.0 41.2 1.0 NH1 U:ARG111 3.1 46.2 1.0 CZ V:TYR69 3.4 50.6 1.0 CE2 V:TYR69 3.6 53.9 1.0 CD U:ARG111 3.6 42.5 1.0 CB U:ASN114 3.7 39.7 1.0 CG U:ASN114 3.7 42.0 1.0 CZ U:ARG111 4.1 45.2 1.0 NE U:ARG111 4.3 45.6 1.0 CA U:ARG111 4.6 39.3 1.0 CE1 V:TYR69 4.7 51.0 1.0 O U:ARG111 4.8 38.9 1.0 CG U:ARG111 4.9 41.0 1.0 OD1 U:ASN114 4.9 46.4 1.0 CD2 V:TYR69 4.9 54.7 1.0

E.M.Huber, W.Heinemeyer, X.Li, C.S.Arendt, M.Hochstrasser, M.Groll. A Unified Mechanism For Proteolysis and Autocatalytic Activation in the 20S Proteasome. Nat Commun V. 7 10900 2016.
ISSN: ESSN 2041-1723
PubMed: 26964885
DOI: 10.1038/NCOMMS10900