Chlorine in PDB 5fga: Yeast 20S Proteasome BETA5-K33A Mutant (Propeptide Expressed in Trans)

All present enzymatic activity of Yeast 20S Proteasome BETA5-K33A Mutant (Propeptide Expressed in Trans):
3.4.25.1;

The structure of Yeast 20S Proteasome BETA5-K33A Mutant (Propeptide Expressed in Trans), PDB code: 5fga was solved by E.M.Huber, M.Groll, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	15.00 / 2.70
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	135.350, 300.500, 144.440, 90.00, 113.10, 90.00
R / Rfree (%)	18.8 / 21.4

The structure of Yeast 20S Proteasome BETA5-K33A Mutant (Propeptide Expressed in Trans) also contains other interesting chemical elements:

Magnesium

(Mg)

8 atoms

The binding sites of Chlorine atom in the Yeast 20S Proteasome BETA5-K33A Mutant (Propeptide Expressed in Trans) (pdb code 5fga). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Yeast 20S Proteasome BETA5-K33A Mutant (Propeptide Expressed in Trans), PDB code: 5fga:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Yeast 20S Proteasome BETA5-K33A Mutant (Propeptide Expressed in Trans)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Yeast 20S Proteasome BETA5-K33A Mutant (Propeptide Expressed in Trans) within 5.0Å range: probe atom residue distance (Å) B Occ G:Cl302 b:39.8 occ:1.00 OH H:TYR69 2.9 46.6 1.0 NH1 G:ARG111 3.0 43.7 1.0 OD1 G:ASN114 3.2 46.1 1.0 CD G:ARG111 3.6 43.4 1.0 CB G:ASN114 3.7 39.9 1.0 CE2 H:TYR69 3.7 41.9 1.0 CZ H:TYR69 3.7 42.1 1.0 CG G:ASN114 3.8 41.8 1.0 CZ G:ARG111 4.0 43.5 1.0 NE G:ARG111 4.2 44.9 1.0 CA G:ARG111 4.7 41.6 1.0 O G:ARG111 4.8 46.6 1.0 CG G:ARG111 4.9 42.4 1.0

Chlorine binding site 2 out of 2 in the Yeast 20S Proteasome BETA5-K33A Mutant (Propeptide Expressed in Trans)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Yeast 20S Proteasome BETA5-K33A Mutant (Propeptide Expressed in Trans) within 5.0Å range: probe atom residue distance (Å) B Occ U:Cl301 b:46.9 occ:1.00 OH V:TYR69 2.9 52.7 1.0 NH1 U:ARG111 3.1 47.5 1.0 OD1 U:ASN114 3.1 52.7 1.0 CB U:ASN114 3.6 45.9 1.0 CE2 V:TYR69 3.7 52.5 1.0 CZ V:TYR69 3.7 51.5 1.0 CG U:ASN114 3.7 47.9 1.0 CD U:ARG111 3.7 47.7 1.0 CZ U:ARG111 4.1 48.0 1.0 NE U:ARG111 4.4 49.3 1.0 CA U:ARG111 4.7 46.3 1.0 O U:ARG111 4.8 45.7 1.0 OD2 O:ASP87 4.9 60.5 1.0 ND2 U:ASN114 4.9 47.4 1.0 CD2 V:TYR69 5.0 52.4 1.0 CE1 V:TYR69 5.0 51.6 1.0

E.M.Huber, W.Heinemeyer, X.Li, C.S.Arendt, M.Hochstrasser, M.Groll. A Unified Mechanism For Proteolysis and Autocatalytic Activation in the 20S Proteasome. Nat Commun V. 7 10900 2016.
ISSN: ESSN 2041-1723
PubMed: 26964885
DOI: 10.1038/NCOMMS10900