Chlorine in PDB 5kop: Arabidopsis Thaliana Fucosyltransferase 1 (FUT1) in Its Apo-Form

All present enzymatic activity of Arabidopsis Thaliana Fucosyltransferase 1 (FUT1) in Its Apo-Form:
2.4.1.69;

The structure of Arabidopsis Thaliana Fucosyltransferase 1 (FUT1) in Its Apo-Form, PDB code: 5kop was solved by J.Rocha, D.De Sanctis, C.Breton, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.66 / 2.10
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	87.838, 85.863, 150.467, 90.00, 96.22, 90.00
R / Rfree (%)	17.7 / 21.5

The binding sites of Chlorine atom in the Arabidopsis Thaliana Fucosyltransferase 1 (FUT1) in Its Apo-Form (pdb code 5kop). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 5 binding sites of Chlorine where determined in the Arabidopsis Thaliana Fucosyltransferase 1 (FUT1) in Its Apo-Form, PDB code: 5kop:
Jump to Chlorine binding site number: 1; 2; 3; 4; 5;

Chlorine binding site 1 out of 5 in the Arabidopsis Thaliana Fucosyltransferase 1 (FUT1) in Its Apo-Form


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Arabidopsis Thaliana Fucosyltransferase 1 (FUT1) in Its Apo-Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl601 b:48.2 occ:1.00 N A:GLY284 3.0 34.3 1.0 CA A:GLY284 3.7 41.2 1.0 CB A:CYS530 3.7 38.3 1.0 N A:GLU283 3.8 40.5 1.0 CB A:CYS282 3.9 38.8 1.0 C A:GLU283 4.1 44.9 1.0 CB A:GLU283 4.3 45.2 1.0 CA A:GLU283 4.3 44.3 1.0 C A:CYS282 4.3 36.9 1.0 O A:CYS530 4.4 53.3 1.0 CA A:CYS282 4.5 35.5 1.0 C A:CYS530 4.6 44.2 1.0 OD2 A:ASP285 4.6 38.3 1.0 N A:ASP285 4.7 45.4 1.0 C A:GLY284 4.7 44.9 1.0 CA A:CYS530 4.7 39.8 1.0 SG A:CYS282 4.8 44.5 1.0 SG A:CYS530 4.9 45.8 1.0 OE1 A:GLU283 5.0 60.4 1.0

Chlorine binding site 2 out of 5 in the Arabidopsis Thaliana Fucosyltransferase 1 (FUT1) in Its Apo-Form


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Arabidopsis Thaliana Fucosyltransferase 1 (FUT1) in Its Apo-Form within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl603 b:51.4 occ:1.00 N C:GLY183 2.9 25.4 1.0 O C:HOH844 3.0 26.3 1.0 CA C:GLY183 3.4 20.0 1.0 N C:LEU182 4.0 24.6 1.0 C C:LEU182 4.0 25.6 1.0 O C:HOH806 4.3 43.9 1.0 CD1 C:PHE484 4.3 27.6 1.0 CA C:LEU182 4.3 24.2 1.0 CB C:LEU182 4.4 22.1 1.0 CB C:PHE484 4.4 25.7 1.0 OG1 C:THR483 4.5 21.1 1.0 C C:GLY181 4.5 24.5 1.0 O C:HOH837 4.6 33.7 1.0 N C:PHE484 4.6 30.7 1.0 C C:GLY183 4.6 21.6 1.0 CA C:PHE484 4.6 25.0 1.0 N C:ASN184 4.8 22.8 1.0 CG C:PHE484 4.9 28.0 1.0 CA C:GLY181 4.9 25.0 1.0 O2 C:EDO602 4.9 60.6 1.0

Chlorine binding site 3 out of 5 in the Arabidopsis Thaliana Fucosyltransferase 1 (FUT1) in Its Apo-Form


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Arabidopsis Thaliana Fucosyltransferase 1 (FUT1) in Its Apo-Form within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl604 b:54.1 occ:1.00 N C:GLY284 3.1 30.4 1.0 CA C:GLY284 3.7 35.5 1.0 CB C:CYS282 3.8 26.9 1.0 N C:GLU283 3.9 33.0 1.0 CB C:CYS530 4.0 38.2 1.0 C C:GLU283 4.2 33.4 1.0 OD2 C:ASP285 4.4 35.3 1.0 O C:HOH760 4.4 33.4 1.0 CA C:GLU283 4.4 38.7 1.0 C C:CYS282 4.4 36.0 1.0 O C:CYS530 4.5 42.2 1.0 CB C:GLU283 4.5 48.0 1.0 CA C:CYS282 4.6 33.4 1.0 N C:ASP285 4.7 32.1 1.0 C C:CYS530 4.7 39.5 1.0 C C:GLY284 4.8 35.9 1.0 SG C:CYS282 4.8 36.5 1.0 CA C:CYS530 4.9 39.7 1.0

Chlorine binding site 4 out of 5 in the Arabidopsis Thaliana Fucosyltransferase 1 (FUT1) in Its Apo-Form


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Arabidopsis Thaliana Fucosyltransferase 1 (FUT1) in Its Apo-Form within 5.0Å range: probe atom residue distance (Å) B Occ D:Cl602 b:49.2 occ:1.00 N D:GLY183 3.0 29.5 1.0 O D:HOH791 3.1 38.4 1.0 CA D:GLY183 3.6 27.0 1.0 N D:LEU182 3.9 25.6 1.0 C D:LEU182 4.1 25.7 1.0 CE D:MET467 4.3 37.2 1.0 CA D:LEU182 4.4 29.3 1.0 CB D:LEU182 4.4 29.5 1.0 CD1 D:PHE484 4.5 27.0 1.0 C D:GLY181 4.5 32.0 1.0 OG1 D:THR483 4.5 30.2 1.0 CB D:PHE484 4.7 21.4 1.0 C D:GLY183 4.8 25.8 1.0 N D:ASN184 4.8 24.4 1.0 N D:PHE484 4.8 20.1 1.0 CA D:GLY181 4.9 37.8 1.0 O D:HOH732 4.9 27.4 1.0 CA D:PHE484 4.9 20.2 1.0

Chlorine binding site 5 out of 5 in the Arabidopsis Thaliana Fucosyltransferase 1 (FUT1) in Its Apo-Form


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 5 of Arabidopsis Thaliana Fucosyltransferase 1 (FUT1) in Its Apo-Form within 5.0Å range: probe atom residue distance (Å) B Occ D:Cl603 b:45.8 occ:1.00 N D:GLY284 3.1 36.7 1.0 CB D:CYS282 3.7 39.5 1.0 CB D:CYS530 3.7 39.5 0.5 CA D:GLY284 3.7 43.6 1.0 N D:GLU283 3.9 44.1 1.0 C D:GLU283 4.1 45.0 1.0 C D:CYS282 4.3 44.3 1.0 CA D:GLU283 4.3 41.3 1.0 CB D:GLU283 4.4 39.0 1.0 CA D:CYS282 4.5 36.6 1.0 OD2 D:ASP285 4.5 41.9 1.0 O D:CYS530 4.5 47.8 0.5 C D:CYS530 4.6 46.5 0.5 CA D:CYS530 4.7 41.9 0.5 SG D:CYS282 4.7 43.5 1.0 N D:ASP285 4.7 43.5 1.0 C D:GLY284 4.8 46.5 1.0 SG D:CYS530 4.9 41.6 0.5 O D:CYS282 4.9 37.5 1.0

J.Rocha, F.Ciceron, D.De Sanctis, M.Lelimousin, V.Chazalet, O.Lerouxel, C.Breton. Structure of Arabidopsis Thaliana FUT1 Reveals A Variant of the Gt-B Class Fold and Provides Insight Into Xyloglucan Fucosylation. Plant Cell V. 28 2352 2016.
ISSN: ESSN 1532-298X
PubMed: 27637560
DOI: 10.1105/TPC.16.00519