Chlorine in PDB 5ltq: Structure of the Yellow Fluorescent Protein Lanyfp From Branchiostoma Lanceolatum at pH 7.5

The structure of Structure of the Yellow Fluorescent Protein Lanyfp From Branchiostoma Lanceolatum at pH 7.5, PDB code: 5ltq was solved by D.Clavel, G.Gotthard, A.Royant, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.29 / 2.05
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	78.660, 197.160, 115.090, 90.00, 90.45, 90.00
R / Rfree (%)	22.5 / 25.4

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 16;

Binding sites:

The binding sites of Chlorine atom in the Structure of the Yellow Fluorescent Protein Lanyfp From Branchiostoma Lanceolatum at pH 7.5 (pdb code 5ltq). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 16 binding sites of Chlorine where determined in the Structure of the Yellow Fluorescent Protein Lanyfp From Branchiostoma Lanceolatum at pH 7.5, PDB code: 5ltq:
Jump to Chlorine binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Chlorine binding site 1 out of 16 in the Structure of the Yellow Fluorescent Protein Lanyfp From Branchiostoma Lanceolatum at pH 7.5


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Structure of the Yellow Fluorescent Protein Lanyfp From Branchiostoma Lanceolatum at pH 7.5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl301 b:29.5 occ:1.00 OG A:SER153 2.8 21.4 1.0 OH A:TYR175 2.9 20.6 1.0 NH2 A:ARG88 3.3 21.0 1.0 NE2 A:HIS62 3.6 21.2 1.0 O A:HOH426 3.6 31.0 1.0 CB A:SER153 3.7 21.4 1.0 CZ A:TYR175 3.8 20.6 1.0 CE1 A:TYR175 3.9 20.7 1.0 CE2 A:PHE155 4.0 21.3 1.0 OG1 A:THR173 4.0 20.5 1.0 CG2 A:THR141 4.1 20.7 1.0 CB A:THR173 4.1 20.6 1.0 CG2 A:THR173 4.4 20.6 1.0 CZ A:ARG88 4.4 21.0 1.0 CD2 A:HIS62 4.4 21.4 1.0 O A:HOH407 4.5 19.9 1.0 CE1 A:HIS62 4.5 21.3 1.0 CD2 A:PHE155 4.6 21.4 1.0 OG1 A:THR141 4.6 20.7 1.0 CD2 A:CR259 4.7 21.7 1.0 NH1 A:ARG195 4.7 22.3 1.0 NE A:ARG88 4.8 20.9 1.0 CB2 A:CR259 4.9 21.6 1.0 CZ A:PHE155 5.0 21.3 1.0 CB A:THR141 5.0 20.8 1.0

Chlorine binding site 2 out of 16 in the Structure of the Yellow Fluorescent Protein Lanyfp From Branchiostoma Lanceolatum at pH 7.5


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Structure of the Yellow Fluorescent Protein Lanyfp From Branchiostoma Lanceolatum at pH 7.5 within 5.0Å range: probe atom residue distance (Å) B Occ D:Cl301 b:33.2 occ:1.00 OG D:SER153 2.8 21.6 1.0 OH D:TYR175 2.9 21.6 1.0 O D:HOH435 3.3 36.9 1.0 NH2 D:ARG88 3.5 21.3 1.0 NE2 D:HIS62 3.5 21.5 1.0 CB D:SER153 3.6 21.7 1.0 CZ D:TYR175 3.9 21.7 1.0 CE1 D:TYR175 4.0 21.9 1.0 OG1 D:THR173 4.0 21.5 1.0 CE1 D:PHE155 4.1 20.6 1.0 CG2 D:THR141 4.1 21.6 1.0 CB D:THR173 4.2 21.7 1.0 CD2 D:HIS62 4.4 21.7 1.0 CE1 D:HIS62 4.4 21.4 1.0 CG2 D:THR173 4.5 21.7 1.0 O D:HOH407 4.5 19.4 1.0 CZ D:ARG88 4.6 21.1 1.0 CD1 D:PHE155 4.6 20.8 1.0 NH1 D:ARG195 4.7 22.8 1.0 CD2 D:CR259 4.7 21.7 1.0 OG1 D:THR141 4.7 21.4 1.0 CB2 D:CR259 4.9 21.4 1.0 NE D:ARG88 4.9 21.1 1.0 NH2 D:ARG195 4.9 22.9 1.0 CB D:THR141 5.0 21.7 1.0

Chlorine binding site 3 out of 16 in the Structure of the Yellow Fluorescent Protein Lanyfp From Branchiostoma Lanceolatum at pH 7.5


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Structure of the Yellow Fluorescent Protein Lanyfp From Branchiostoma Lanceolatum at pH 7.5 within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl301 b:34.9 occ:1.00 OH B:TYR175 2.9 25.4 1.0 OG B:SER153 2.9 23.8 1.0 O B:HOH416 3.2 31.3 1.0 NH2 B:ARG88 3.4 23.2 1.0 NE2 B:HIS62 3.7 28.3 1.0 CB B:SER153 3.7 23.6 1.0 CZ B:TYR175 3.9 25.4 1.0 CE1 B:TYR175 3.9 25.2 1.0 CE2 B:PHE155 4.0 22.5 1.0 OG1 B:THR173 4.0 23.0 1.0 CB B:THR173 4.1 23.3 1.0 CG2 B:THR141 4.1 22.7 1.0 CG2 B:THR173 4.4 23.5 1.0 CZ B:ARG88 4.5 23.1 1.0 O B:HOH408 4.5 25.5 1.0 CE1 B:HIS62 4.6 28.6 1.0 CD2 B:PHE155 4.6 22.5 1.0 CD2 B:HIS62 4.6 28.6 1.0 OG1 B:THR141 4.6 22.7 1.0 CD2 B:CR259 4.6 27.1 1.0 NH1 B:ARG195 4.7 26.6 1.0 CB2 B:CR259 4.8 27.4 1.0 NE B:ARG88 4.8 22.8 1.0 CB B:THR141 5.0 22.8 1.0 NH2 B:ARG195 5.0 27.1 1.0

Chlorine binding site 4 out of 16 in the Structure of the Yellow Fluorescent Protein Lanyfp From Branchiostoma Lanceolatum at pH 7.5


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Structure of the Yellow Fluorescent Protein Lanyfp From Branchiostoma Lanceolatum at pH 7.5 within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl301 b:33.4 occ:1.00 OH C:TYR175 2.9 22.6 1.0 OG C:SER153 3.1 21.9 1.0 O C:HOH439 3.2 32.3 1.0 NH2 C:ARG88 3.3 21.6 1.0 NE2 C:HIS62 3.6 23.3 1.0 CB C:SER153 3.6 21.8 1.0 CZ C:TYR175 3.9 22.7 1.0 CE1 C:TYR175 4.0 22.7 1.0 CE1 C:PHE155 4.0 20.7 1.0 OG1 C:THR173 4.1 21.7 1.0 CG2 C:THR141 4.1 20.6 1.0 CB C:THR173 4.2 21.9 1.0 CD2 C:HIS62 4.4 23.6 1.0 CZ C:ARG88 4.5 21.5 1.0 CG2 C:THR173 4.5 22.1 1.0 CE1 C:HIS62 4.5 23.4 1.0 CD2 C:CR259 4.6 22.4 1.0 OG1 C:THR141 4.6 20.4 1.0 O C:HOH412 4.6 18.1 1.0 CD1 C:PHE155 4.6 20.7 1.0 NH1 C:ARG195 4.7 22.5 1.0 CB2 C:CR259 4.8 22.6 1.0 NE C:ARG88 4.8 21.4 1.0 CB C:THR141 4.9 20.6 1.0 NH2 C:ARG195 4.9 22.9 1.0 CZ C:PHE155 5.0 20.6 1.0

Chlorine binding site 5 out of 16 in the Structure of the Yellow Fluorescent Protein Lanyfp From Branchiostoma Lanceolatum at pH 7.5


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 5 of Structure of the Yellow Fluorescent Protein Lanyfp From Branchiostoma Lanceolatum at pH 7.5 within 5.0Å range: probe atom residue distance (Å) B Occ E:Cl301 b:36.9 occ:1.00 OG E:SER153 2.9 25.0 1.0 OH E:TYR175 3.0 26.3 1.0 NH2 E:ARG88 3.5 25.4 1.0 NE2 E:HIS62 3.6 29.4 1.0 CB E:SER153 3.8 25.0 1.0 CZ E:TYR175 3.9 26.5 1.0 CE1 E:TYR175 3.9 26.3 1.0 CE1 E:PHE155 4.0 23.3 1.0 CG2 E:THR141 4.1 24.6 1.0 OG1 E:THR173 4.1 24.6 1.0 CB E:THR173 4.2 25.0 1.0 CD2 E:HIS62 4.4 29.9 1.0 O E:HOH414 4.6 26.1 1.0 CD1 E:PHE155 4.6 23.2 1.0 CG2 E:THR173 4.6 25.4 1.0 CZ E:ARG88 4.6 25.2 1.0 CE1 E:HIS62 4.6 29.8 1.0 OG1 E:THR141 4.7 24.5 1.0 NH1 E:ARG195 4.7 27.6 1.0 CD2 E:CR259 4.7 27.9 1.0 CB2 E:CR259 4.9 28.5 1.0 NE E:ARG88 4.9 24.8 1.0 CB E:THR141 4.9 24.6 1.0 NH2 E:ARG195 4.9 28.6 1.0

Chlorine binding site 6 out of 16 in the Structure of the Yellow Fluorescent Protein Lanyfp From Branchiostoma Lanceolatum at pH 7.5


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 6 of Structure of the Yellow Fluorescent Protein Lanyfp From Branchiostoma Lanceolatum at pH 7.5 within 5.0Å range: probe atom residue distance (Å) B Occ H:Cl301 b:39.9 occ:1.00 OH H:TYR175 2.9 29.2 1.0 OG H:SER153 3.0 26.6 1.0 O H:HOH411 3.2 40.7 1.0 NH2 H:ARG88 3.5 25.9 1.0 NE2 H:HIS62 3.7 31.2 1.0 CB H:SER153 3.7 26.8 1.0 CZ H:TYR175 3.8 29.5 1.0 CE1 H:TYR175 3.8 29.3 1.0 OG1 H:THR173 4.0 25.9 1.0 CG2 H:THR141 4.1 25.5 1.0 CE1 H:PHE155 4.1 24.8 1.0 CB H:THR173 4.1 26.4 1.0 CG2 H:THR173 4.4 26.9 1.0 CE1 H:HIS62 4.5 31.5 1.0 CZ H:ARG88 4.6 25.6 1.0 CD2 H:HIS62 4.6 31.9 1.0 NH1 H:ARG195 4.7 28.9 1.0 O H:HOH408 4.7 21.6 1.0 CD2 H:CR259 4.7 27.6 1.0 CD1 H:PHE155 4.7 24.7 1.0 OG1 H:THR141 4.7 25.4 1.0 CB2 H:CR259 4.8 28.4 1.0 NH2 H:ARG195 4.9 30.0 1.0 NE H:ARG88 4.9 25.1 1.0 CB H:THR141 5.0 25.5 1.0

Chlorine binding site 7 out of 16 in the Structure of the Yellow Fluorescent Protein Lanyfp From Branchiostoma Lanceolatum at pH 7.5


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 7 of Structure of the Yellow Fluorescent Protein Lanyfp From Branchiostoma Lanceolatum at pH 7.5 within 5.0Å range: probe atom residue distance (Å) B Occ F:Cl301 b:32.4 occ:1.00 OH F:TYR175 2.9 25.1 1.0 OG F:SER153 2.9 23.1 1.0 NH2 F:ARG88 3.2 25.5 1.0 O F:HOH411 3.5 34.3 1.0 NE2 F:HIS62 3.6 26.1 1.0 CE1 F:TYR175 3.8 24.8 1.0 CZ F:TYR175 3.8 25.1 1.0 CB F:SER153 3.9 23.0 1.0 OG1 F:THR173 3.9 24.0 1.0 CB F:THR173 4.1 24.2 1.0 CG2 F:THR141 4.1 22.9 1.0 CE1 F:PHE155 4.2 22.8 1.0 O F:HOH403 4.2 22.8 1.0 CZ F:ARG88 4.3 25.3 1.0 CG2 F:THR173 4.4 24.5 1.0 CE1 F:HIS62 4.5 26.4 1.0 CD2 F:HIS62 4.5 26.5 1.0 NE F:ARG88 4.7 25.0 1.0 CD2 F:CR259 4.8 25.6 1.0 CD1 F:PHE155 4.8 22.7 1.0 CB2 F:CR259 4.9 26.1 1.0 OG1 F:THR141 4.9 23.1 1.0 NH1 F:ARG195 4.9 26.3 1.0

Chlorine binding site 8 out of 16 in the Structure of the Yellow Fluorescent Protein Lanyfp From Branchiostoma Lanceolatum at pH 7.5


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 8 of Structure of the Yellow Fluorescent Protein Lanyfp From Branchiostoma Lanceolatum at pH 7.5 within 5.0Å range: probe atom residue distance (Å) B Occ G:Cl301 b:33.9 occ:1.00 OH G:TYR175 3.0 22.3 1.0 NH2 G:ARG88 3.2 24.4 1.0 O G:HOH441 3.2 32.8 1.0 CB G:SER153 3.6 22.3 1.0 NE2 G:HIS62 3.6 26.3 1.0 OG G:SER153 3.7 22.6 1.0 CZ G:TYR175 3.9 22.3 1.0 CE1 G:TYR175 3.9 22.2 1.0 CE2 G:PHE155 4.0 23.1 1.0 CG2 G:THR141 4.1 22.1 1.0 OG1 G:THR173 4.1 22.6 1.0 CB G:THR173 4.2 22.5 1.0 CZ G:ARG88 4.3 24.4 1.0 O G:HOH414 4.4 23.6 1.0 CD2 G:HIS62 4.5 26.5 1.0 CG2 G:THR173 4.5 22.7 1.0 CD2 G:PHE155 4.6 23.2 1.0 OG1 G:THR141 4.6 22.4 1.0 CD2 G:CR259 4.6 26.9 1.0 CE1 G:HIS62 4.6 26.6 1.0 NH1 G:ARG195 4.6 25.4 1.0 NE G:ARG88 4.7 24.3 1.0 CB2 G:CR259 4.8 27.0 1.0 CB G:THR141 4.9 22.4 1.0 NH2 G:ARG195 5.0 25.8 1.0 CZ G:PHE155 5.0 23.3 1.0

Chlorine binding site 9 out of 16 in the Structure of the Yellow Fluorescent Protein Lanyfp From Branchiostoma Lanceolatum at pH 7.5


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 9 of Structure of the Yellow Fluorescent Protein Lanyfp From Branchiostoma Lanceolatum at pH 7.5 within 5.0Å range: probe atom residue distance (Å) B Occ I:Cl301 b:49.1 occ:1.00 OH I:TYR175 2.9 35.8 1.0 OG I:SER153 2.9 34.6 1.0 O I:HOH408 3.4 36.8 1.0 NH2 I:ARG88 3.5 37.7 1.0 NE2 I:HIS62 3.9 42.7 1.0 CE1 I:PHE155 3.9 36.0 1.0 CG2 I:THR141 3.9 38.1 1.0 OG1 I:THR173 3.9 34.2 1.0 CZ I:TYR175 3.9 35.2 1.0 CB I:SER153 3.9 34.4 1.0 CE1 I:TYR175 4.0 34.4 1.0 CB I:THR173 4.1 33.8 1.0 CZ I:ARG88 4.5 37.5 1.0 CG2 I:THR173 4.5 34.2 1.0 CD1 I:PHE155 4.5 35.9 1.0 OG1 I:THR141 4.6 39.6 1.0 CD2 I:CR259 4.7 43.5 1.0 CE1 I:HIS62 4.7 43.7 1.0 NE I:ARG88 4.7 36.5 1.0 CD2 I:HIS62 4.8 42.7 1.0 CZ I:PHE155 4.8 36.5 1.0 CB I:THR141 4.8 39.0 1.0 NH1 I:ARG195 4.8 44.7 1.0 CB2 I:CR259 4.9 44.1 1.0

Chlorine binding site 10 out of 16 in the Structure of the Yellow Fluorescent Protein Lanyfp From Branchiostoma Lanceolatum at pH 7.5


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 10 of Structure of the Yellow Fluorescent Protein Lanyfp From Branchiostoma Lanceolatum at pH 7.5 within 5.0Å range: probe atom residue distance (Å) B Occ L:Cl301 b:40.0 occ:1.00 OH L:TYR175 2.9 26.1 1.0 OG L:SER153 2.9 25.0 1.0 NH2 L:ARG88 3.3 28.3 1.0 O L:HOH427 3.3 35.2 1.0 CB L:SER153 3.6 24.6 1.0 NE2 L:HIS62 3.6 27.5 1.0 CZ L:TYR175 3.8 26.3 1.0 CE1 L:TYR175 3.8 26.0 1.0 OG1 L:THR173 4.0 27.0 1.0 CE2 L:PHE155 4.0 23.7 1.0 CB L:THR173 4.1 27.0 1.0 CG2 L:THR141 4.1 23.6 1.0 CZ L:ARG88 4.4 28.5 1.0 CG2 L:THR173 4.4 27.2 1.0 CE1 L:HIS62 4.5 27.8 1.0 CD2 L:HIS62 4.5 27.7 1.0 O L:HOH431 4.6 27.2 1.0 CD2 L:PHE155 4.7 23.6 1.0 NE L:ARG88 4.8 28.4 1.0 CD2 L:CR259 4.8 28.3 1.0 CB2 L:CR259 4.9 28.9 1.0 OG1 L:THR141 4.9 23.9 1.0 NH1 L:ARG195 4.9 26.0 1.0 CZ L:PHE155 5.0 24.1 1.0

D.Clavel, G.Gotthard, D.Von Stetten, D.De Sanctis, H.Pasquier, G.G.Lambert, N.C.Shaner, A.Royant. Structural Analysis of the Bright Monomeric Yellow-Green Fluorescent Protein Mneongreen Obtained By Directed Evolution. Acta Crystallogr D Struct V. 72 1298 2016BIOL.
ISSN: ISSN 2059-7983
PubMed: 27917830
DOI: 10.1107/S2059798316018623