Chlorine in PDB 5luh: Aada E87Q in Complex with Atp, Calcium and Streptomycin

All present enzymatic activity of Aada E87Q in Complex with Atp, Calcium and Streptomycin:
2.7.7.47;

The structure of Aada E87Q in Complex with Atp, Calcium and Streptomycin, PDB code: 5luh was solved by A.L.Stern, S.E.Van Der Verren, M.Selmer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	36.57 / 1.73
Space group	P 32
Cell size a, b, c (Å), α, β, γ (°)	82.472, 82.472, 79.174, 90.00, 90.00, 120.00
R / Rfree (%)	18.9 / 22.6

The structure of Aada E87Q in Complex with Atp, Calcium and Streptomycin also contains other interesting chemical elements:

Calcium

(Ca)

4 atoms

The binding sites of Chlorine atom in the Aada E87Q in Complex with Atp, Calcium and Streptomycin (pdb code 5luh). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Aada E87Q in Complex with Atp, Calcium and Streptomycin, PDB code: 5luh:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Aada E87Q in Complex with Atp, Calcium and Streptomycin


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Aada E87Q in Complex with Atp, Calcium and Streptomycin within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl307 b:18.5 occ:1.00 O A:HOH460 2.9 27.9 1.0 N A:GLY41 3.1 19.3 1.0 N A:LEU42 3.4 13.6 1.0 CA A:GLY41 3.8 15.3 1.0 C A:GLY41 4.1 15.3 1.0 C A:GLY40 4.1 13.0 1.0 CA A:GLY40 4.1 15.5 1.0 CB A:LEU42 4.3 19.1 1.0 O A:HOH605 4.3 29.5 1.0 CA A:LEU42 4.4 18.8 1.0 O A:LEU42 4.6 22.0 1.0 C A:LEU42 5.0 18.1 1.0 O A:SER36 5.0 15.7 1.0

Chlorine binding site 2 out of 2 in the Aada E87Q in Complex with Atp, Calcium and Streptomycin


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Aada E87Q in Complex with Atp, Calcium and Streptomycin within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl305 b:19.1 occ:1.00 O B:HOH454 3.0 30.2 1.0 N B:GLY41 3.2 17.2 1.0 O B:HOH497 3.2 21.7 1.0 N B:LEU42 3.3 15.8 1.0 CA B:GLY41 3.9 16.1 1.0 C B:GLY40 4.1 17.9 1.0 C B:GLY41 4.1 13.6 1.0 CA B:GLY40 4.1 17.3 1.0 CB B:LEU42 4.2 21.4 1.0 CA B:LEU42 4.3 16.2 1.0 O B:HOH604 4.4 29.9 1.0 O B:LEU42 4.6 21.7 1.0 C B:LEU42 4.9 16.8 1.0 O B:SER36 4.9 14.5 1.0

A.L.Stern, S.E.Van Der Verren, S.Kanchugal P, J.Nasvall, H.Gutierrez-De-Teran, M.Selmer. Structural Mechanism of Aada, A Dual-Specificity Aminoglycoside Adenylyltransferase Fromsalmonella Enterica. J.Biol.Chem. V. 293 11481 2018.
ISSN: ESSN 1083-351X
PubMed: 29871922
DOI: 10.1074/JBC.RA118.003989