Chlorine in PDB 5lvg: Hen Egg White Lysozyme Soaked with Cis-Ru(Dmso)4CL2

All present enzymatic activity of Hen Egg White Lysozyme Soaked with Cis-Ru(Dmso)4CL2:
3.2.1.17;

The structure of Hen Egg White Lysozyme Soaked with Cis-Ru(Dmso)4CL2, PDB code: 5lvg was solved by K.Kurpiewska, P.Halajko, K.Lewinski, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	14.42 / 2.00
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	78.199, 78.199, 37.189, 90.00, 90.00, 90.00
R / Rfree (%)	15.8 / 23.3

The structure of Hen Egg White Lysozyme Soaked with Cis-Ru(Dmso)4CL2 also contains other interesting chemical elements:

Ruthenium	(Ru)	1 atom
Sodium	(Na)	1 atom

The binding sites of Chlorine atom in the Hen Egg White Lysozyme Soaked with Cis-Ru(Dmso)4CL2 (pdb code 5lvg). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the Hen Egg White Lysozyme Soaked with Cis-Ru(Dmso)4CL2, PDB code: 5lvg:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in the Hen Egg White Lysozyme Soaked with Cis-Ru(Dmso)4CL2


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Hen Egg White Lysozyme Soaked with Cis-Ru(Dmso)4CL2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl202 b:22.6 occ:1.00 OG A:SER24 3.0 11.8 1.0 N A:GLY26 3.1 10.8 1.0 CA A:GLY26 3.4 10.0 1.0 CB A:SER24 3.5 12.2 1.0 CA A:GLN121 3.7 15.5 1.0 N A:GLN121 4.0 13.9 1.0 CB A:GLN121 4.0 20.3 1.0 CD1 A:ILE124 4.0 19.2 1.0 N A:LEU25 4.2 11.5 1.0 CG1 A:ILE124 4.2 16.6 1.0 CG2 A:VAL120 4.2 12.6 1.0 O A:VAL120 4.2 11.0 1.0 C A:LEU25 4.3 11.2 1.0 C A:VAL120 4.3 13.4 1.0 CG A:GLN121 4.3 25.5 1.0 C A:SER24 4.3 11.3 1.0 CA A:SER24 4.5 11.1 1.0 C A:GLY26 4.5 9.0 1.0 N A:ASN27 4.7 8.1 1.0 CA A:LEU25 4.7 11.8 1.0 O A:SER24 4.8 9.7 1.0 C A:GLN121 4.9 13.8 1.0

Chlorine binding site 2 out of 4 in the Hen Egg White Lysozyme Soaked with Cis-Ru(Dmso)4CL2


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Hen Egg White Lysozyme Soaked with Cis-Ru(Dmso)4CL2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl203 b:28.6 occ:1.00 OD1 A:ASN65 3.2 17.7 1.0 O A:HOH394 3.3 15.1 1.0 CB A:PRO79 3.7 10.8 1.0 CA A:PRO79 3.8 10.9 1.0 CB A:ASN65 3.9 11.6 1.0 CG A:ASN65 4.0 12.8 1.0 N A:PRO79 4.1 11.0 1.0 O A:ASN65 4.3 8.7 1.0 CG A:PRO79 4.4 12.0 1.0 CD A:PRO79 4.4 11.5 1.0 C A:ILE78 4.6 11.4 1.0 O A:ILE78 4.8 9.7 1.0 O A:HOH387 4.9 11.6 1.0 C A:ASN65 4.9 9.3 1.0 CA A:ASN65 5.0 10.1 1.0

Chlorine binding site 3 out of 4 in the Hen Egg White Lysozyme Soaked with Cis-Ru(Dmso)4CL2


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Hen Egg White Lysozyme Soaked with Cis-Ru(Dmso)4CL2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl204 b:28.9 occ:0.80 RU A:RU201 2.8 24.5 0.8 O A:HOH330 2.9 31.6 0.8 NE2 A:HIS15 3.5 22.3 1.0 N A:ILE88 3.5 13.8 1.0 NH1 A:ARG14 3.7 21.8 0.8 CG1 A:ILE88 3.8 12.6 1.0 OD1 A:ASP87 4.0 20.8 1.0 CA A:ASP87 4.1 16.7 1.0 CD2 A:HIS15 4.1 22.6 1.0 NH2 A:ARG14 4.1 21.2 0.8 O A:HOH316 4.3 28.1 1.0 C A:ASP87 4.3 14.2 1.0 CB A:ALA11 4.4 11.8 1.0 CZ A:ARG14 4.4 21.0 0.8 CB A:ILE88 4.5 12.7 1.0 O A:SER86 4.5 21.6 1.0 CA A:ILE88 4.5 12.6 1.0 CE1 A:HIS15 4.5 22.6 1.0 CG2 A:ILE88 4.6 12.6 1.0 CD1 A:ILE88 4.6 12.7 1.0 CG A:ASP87 4.6 22.0 1.0 CB A:ASP87 4.8 18.6 1.0 N A:THR89 4.9 13.5 1.0

Chlorine binding site 4 out of 4 in the Hen Egg White Lysozyme Soaked with Cis-Ru(Dmso)4CL2


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Hen Egg White Lysozyme Soaked with Cis-Ru(Dmso)4CL2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl205 b:25.3 occ:1.00 O A:HOH371 0.8 16.1 1.0 OH A:TYR23 3.0 8.2 1.0 O A:HOH335 3.1 13.6 1.0 O A:HOH395 3.1 28.2 1.0 CZ A:TYR23 3.6 8.8 1.0 CE2 A:TYR23 3.7 8.4 1.0 O A:ARG21 4.4 11.9 1.0 CA A:GLY104 4.5 10.0 1.0 CE1 A:TYR23 4.7 8.9 1.0 CD2 A:TYR23 4.9 8.5 1.0

M.Oszajca, K.Kurpiewska, P.Halajko, A.Szura, K.Lewinski, M.Brindell. Ligand-Dependent Interaction of Anticancer Ruthenium Complexes with Hen Egg White Lysozyme and Human Lysozyme To Be Published.