Chlorine in PDB 5ox8: Structure of Enhanced Cyan Fluorescent Protein at pH 5.0

The structure of Structure of Enhanced Cyan Fluorescent Protein at pH 5.0, PDB code: 5ox8 was solved by G.Gotthard, D.Von Stetten, D.Clavel, M.Noirclerc-Savoye, A.Royant, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.66 / 1.29
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	51.247, 62.624, 69.108, 90.00, 90.00, 90.00
R / Rfree (%)	13.2 / 16.6

The binding sites of Chlorine atom in the Structure of Enhanced Cyan Fluorescent Protein at pH 5.0 (pdb code 5ox8). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the Structure of Enhanced Cyan Fluorescent Protein at pH 5.0, PDB code: 5ox8:

Chlorine binding site 1 out of 1 in the Structure of Enhanced Cyan Fluorescent Protein at pH 5.0


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Structure of Enhanced Cyan Fluorescent Protein at pH 5.0 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl301 b:24.0 occ:1.00 O A:HOH535 2.9 38.5 1.0 O A:HOH542 3.0 21.5 1.0 O A:HOH591 3.0 28.3 1.0 N A:TRP57 3.1 13.7 1.0 O A:HOH506 3.3 27.7 1.0 CD1 A:TRP57 3.7 15.4 1.0 CD A:PRO58 3.8 14.3 1.0 CA A:PRO56 3.8 14.2 1.0 CB A:TRP57 3.8 14.4 1.0 C A:PRO56 3.9 13.4 1.0 CD1 A:TYR143 4.0 16.4 1.0 CA A:TRP57 4.0 14.6 1.0 CG A:TRP57 4.1 14.6 1.0 CB A:PRO56 4.2 15.8 1.0 O A:HOH559 4.2 37.8 1.0 O A:HOH530 4.4 19.5 1.0 CE1 A:TYR143 4.4 16.7 1.0 O A:HOH674 4.7 46.1 1.0 N A:PRO58 4.8 14.3 1.0 CG A:PRO58 4.9 15.6 1.0 NE1 A:TRP57 4.9 15.7 1.0 O A:HOH671 4.9 35.3 1.0 C A:TRP57 5.0 13.8 1.0 CG A:TYR143 5.0 16.3 1.0 O A:VAL55 5.0 16.9 1.0

G.Gotthard, D.Von Stetten, D.Clavel, M.Noirclerc-Savoye, A.Royant. Chromophore Isomer Stabilization Is Critical to the Efficient Fluorescence of Cyan Fluorescent Proteins. Biochemistry V. 56 6418 2017.
ISSN: ISSN 1520-4995
PubMed: 29148725
DOI: 10.1021/ACS.BIOCHEM.7B01088