Chlorine in PDB 5vzb: Post-Catalytic Complex of Human Polymerase Mu (G433S) Mutant with Incoming Utp

All present enzymatic activity of Post-Catalytic Complex of Human Polymerase Mu (G433S) Mutant with Incoming Utp:
2.7.7.7;

The structure of Post-Catalytic Complex of Human Polymerase Mu (G433S) Mutant with Incoming Utp, PDB code: 5vzb was solved by A.F.Moon, J.M.Pryor, D.A.Ramsden, T.A.Kunkel, K.Bebenek, L.C.Pedersen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	32.33 / 1.50
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	59.807, 68.843, 109.848, 90.00, 90.00, 90.00
R / Rfree (%)	17.1 / 18.7

The structure of Post-Catalytic Complex of Human Polymerase Mu (G433S) Mutant with Incoming Utp also contains other interesting chemical elements:

Magnesium	(Mg)	3 atoms
Sodium	(Na)	1 atom

The binding sites of Chlorine atom in the Post-Catalytic Complex of Human Polymerase Mu (G433S) Mutant with Incoming Utp (pdb code 5vzb). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Post-Catalytic Complex of Human Polymerase Mu (G433S) Mutant with Incoming Utp, PDB code: 5vzb:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Post-Catalytic Complex of Human Polymerase Mu (G433S) Mutant with Incoming Utp


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Post-Catalytic Complex of Human Polymerase Mu (G433S) Mutant with Incoming Utp within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl504 b:31.1 occ:1.00 O A:HOH698 2.8 12.6 1.0 N A:GLU485 2.9 13.1 0.3 N A:GLU485 2.9 12.9 0.7 O A:HOH844 2.9 21.2 0.6 CB A:GLU485 3.6 14.9 0.7 CB A:GLU485 3.6 14.8 0.3 CA A:LEU484 3.7 12.1 1.0 CG A:GLU485 3.7 17.1 0.7 C A:LEU484 3.8 13.0 1.0 CA A:GLU485 3.8 13.6 0.3 CA A:GLU485 3.8 13.5 0.7 CD2 A:LEU484 3.9 13.0 1.0 O A:GLN144 3.9 16.2 1.0 O A:HOH840 3.9 17.3 1.0 CB A:LEU484 4.0 11.1 1.0 CA A:GLN144 4.1 12.9 1.0 O A:GLU485 4.3 11.8 0.7 O A:GLU485 4.3 11.9 0.3 C A:GLN144 4.3 14.1 1.0 CB A:GLN144 4.5 13.5 1.0 C A:GLU485 4.6 12.9 0.7 C A:GLU485 4.6 12.9 0.3 CG A:LEU484 4.6 11.6 1.0 O A:HOH844 4.7 26.5 0.5 O A:CYS143 4.7 14.3 1.0 O A:GLY483 4.8 17.4 1.0 CG A:GLN144 4.8 12.7 1.0 N A:LEU484 5.0 11.6 1.0 O A:LEU484 5.0 14.8 1.0

Chlorine binding site 2 out of 2 in the Post-Catalytic Complex of Human Polymerase Mu (G433S) Mutant with Incoming Utp


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Post-Catalytic Complex of Human Polymerase Mu (G433S) Mutant with Incoming Utp within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl505 b:17.4 occ:0.58 O2 T:DC4 2.8 12.9 1.0 NH2 A:ARG181 2.9 12.5 1.0 O T:HOH222 2.9 24.8 1.0 NH1 A:ARG181 2.9 12.3 1.0 O4' T:DC4 3.3 14.1 1.0 CZ A:ARG181 3.3 10.4 1.0 C1' T:DC4 3.5 12.8 1.0 N2 T:DG3 3.5 14.9 1.0 C2 T:DC4 3.7 13.4 1.0 C4' T:DC4 3.9 12.7 1.0 O D:HOH116 4.0 23.0 1.0 N3 T:DG3 4.0 14.8 1.0 N1 T:DC4 4.0 13.5 1.0 NH1 A:ARG446 4.0 21.9 1.0 C2 T:DG3 4.1 14.7 1.0 O A:HOH658 4.1 16.4 0.4 N2 D:DG1 4.2 12.9 1.0 CD A:ARG446 4.3 14.5 1.0 CZ A:ARG446 4.3 15.8 1.0 O A:HOH811 4.4 31.6 1.0 NE A:ARG446 4.5 14.2 1.0 NE A:ARG181 4.6 10.5 1.0 O D:HOH107 4.8 31.1 1.0 C2' T:DC4 4.8 12.5 1.0 C3' T:DC4 4.8 12.2 1.0 N3 T:DC4 4.8 14.0 1.0 O3' T:DC4 4.8 11.6 1.0 CD1 A:LEU177 4.9 14.5 1.0

A.F.Moon, J.M.Pryor, D.A.Ramsden, T.A.Kunkel, K.Bebenek, L.C.Pedersen. Structural Accommodation of Ribonucleotide Incorporation By the Dna Repair Enzyme Polymerase Mu. Nucleic Acids Res. V. 45 9138 2017.
ISSN: ESSN 1362-4962
PubMed: 28911097
DOI: 10.1093/NAR/GKX527