Chlorine in PDB 5w2j: Crystal Structure of Dimeric Form of Mouse Glutaminase C

All present enzymatic activity of Crystal Structure of Dimeric Form of Mouse Glutaminase C:
3.5.1.2;

The structure of Crystal Structure of Dimeric Form of Mouse Glutaminase C, PDB code: 5w2j was solved by R.A.Cerione, Y.Li, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.50
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	84.940, 100.880, 145.450, 90.00, 90.00, 90.00
R / Rfree (%)	19.6 / 22.8

The binding sites of Chlorine atom in the Crystal Structure of Dimeric Form of Mouse Glutaminase C (pdb code 5w2j). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of Dimeric Form of Mouse Glutaminase C, PDB code: 5w2j:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Crystal Structure of Dimeric Form of Mouse Glutaminase C


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of Dimeric Form of Mouse Glutaminase C within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl601 b:62.5 occ:1.00 O A:HOH753 2.7 53.2 1.0 N A:VAL489 2.9 42.0 1.0 OG A:SER291 3.0 37.9 1.0 CB A:SER291 3.2 39.2 1.0 N A:SER291 3.2 38.1 1.0 CB A:VAL489 3.3 44.7 1.0 CB A:GLN290 3.5 40.6 1.0 OH A:TYR471 3.6 72.2 1.0 CG1 A:VAL489 3.6 38.8 1.0 CA A:GLY488 3.7 40.8 1.0 CA A:VAL489 3.7 42.2 1.0 C A:GLY488 3.7 43.3 1.0 CA A:SER291 3.8 36.7 1.0 OE1 A:GLN290 4.2 53.3 1.0 C A:GLN290 4.2 39.2 1.0 CG A:GLN290 4.4 45.7 1.0 CA A:GLN290 4.4 40.3 1.0 CZ A:TYR471 4.6 78.2 1.0 CG2 A:VAL489 4.6 44.2 1.0 CD A:GLN290 4.7 49.7 1.0 O A:GLY488 4.9 46.4 1.0 C A:VAL489 4.9 41.2 1.0 CE1 A:TYR471 4.9 66.3 1.0 N A:GLY488 4.9 39.9 1.0 O A:SER487 4.9 40.4 1.0

Chlorine binding site 2 out of 2 in the Crystal Structure of Dimeric Form of Mouse Glutaminase C


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of Dimeric Form of Mouse Glutaminase C within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl601 b:82.0 occ:1.00 N B:SER291 2.9 53.0 1.0 OG B:SER291 2.9 52.6 1.0 CB B:SER291 2.9 49.6 1.0 N B:VAL489 3.0 54.9 1.0 OH B:TYR471 3.3 92.7 1.0 CA B:SER291 3.4 52.2 1.0 CA B:GLY488 3.5 45.9 1.0 CB B:GLN290 3.5 57.6 1.0 CB B:VAL489 3.6 62.5 1.0 C B:GLY488 3.8 50.7 1.0 CG1 B:VAL489 3.8 62.5 1.0 C B:GLN290 4.0 54.6 1.0 CA B:VAL489 4.0 55.9 1.0 CA B:GLN290 4.3 54.1 1.0 CZ B:TYR471 4.5 83.0 1.0 C B:SER291 4.6 47.0 1.0 CG B:GLN290 4.6 61.1 1.0 OE1 B:GLN290 4.7 69.6 1.0 O B:SER487 4.7 41.4 1.0 N B:GLY488 4.8 44.5 1.0 CG2 B:VAL489 4.9 64.0 1.0

Y.Li, J.W.Erickson, C.A.Stalnecker, W.P.Katt, Q.Huang, R.A.Cerione, S.Ramachandran. Mechanistic Basis of Glutaminase Activation: A Key Enzyme That Promotes Glutamine Metabolism in Cancer Cells. J. Biol. Chem. V. 291 20900 2016.
ISSN: ESSN 1083-351X
PubMed: 27542409
DOI: 10.1074/JBC.M116.720268