Chlorine in PDB 5xh7: Crystal Structure of the Acidaminococcus Sp. BV3L6 CPF1 Rr Variant in Complex with Crrna and Target Dna (Tcca Pam)

The structure of Crystal Structure of the Acidaminococcus Sp. BV3L6 CPF1 Rr Variant in Complex with Crrna and Target Dna (Tcca Pam), PDB code: 5xh7 was solved by H.Nishimasu, T.Yamano, R.Ishitani, O.Nureki, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.00 / 2.00
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	80.656, 133.298, 200.020, 90.00, 90.00, 90.00
R / Rfree (%)	18.3 / 21.4

The structure of Crystal Structure of the Acidaminococcus Sp. BV3L6 CPF1 Rr Variant in Complex with Crrna and Target Dna (Tcca Pam) also contains other interesting chemical elements:

Sodium

(Na)

5 atoms

The binding sites of Chlorine atom in the Crystal Structure of the Acidaminococcus Sp. BV3L6 CPF1 Rr Variant in Complex with Crrna and Target Dna (Tcca Pam) (pdb code 5xh7). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the Crystal Structure of the Acidaminococcus Sp. BV3L6 CPF1 Rr Variant in Complex with Crrna and Target Dna (Tcca Pam), PDB code: 5xh7:

Chlorine binding site 1 out of 1 in the Crystal Structure of the Acidaminococcus Sp. BV3L6 CPF1 Rr Variant in Complex with Crrna and Target Dna (Tcca Pam)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of the Acidaminococcus Sp. BV3L6 CPF1 Rr Variant in Complex with Crrna and Target Dna (Tcca Pam) within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1402 b:49.6 occ:1.00 N A:LYS1072 3.1 30.9 1.0 N A:SER1071 3.2 31.9 1.0 N A:TYR1069 3.4 32.1 1.0 N A:THR1070 3.5 32.4 1.0 CA A:TYR1069 3.7 32.1 1.0 CA A:SER1071 3.8 33.4 1.0 CB A:SER1071 3.8 44.1 1.0 CB A:LYS1072 3.9 25.6 1.0 C A:SER1071 3.9 33.0 1.0 C A:TYR1069 4.0 33.4 1.0 CA A:LYS1072 4.1 36.2 1.0 CG A:LYS1072 4.1 32.6 1.0 C A:THR1070 4.2 35.4 1.0 CA A:THR1070 4.4 34.4 1.0 C A:PRO1068 4.5 42.3 1.0 O A:ALA1067 4.5 33.7 1.0 OG1 A:THR1070 4.6 33.8 1.0 O A:HOH1837 4.6 45.8 1.0 C A:LYS1072 4.7 34.1 1.0 CD A:LYS1072 4.8 36.1 1.0 CA A:PRO1068 4.8 38.5 1.0 OG A:SER1071 4.9 55.7 1.0

H.Nishimasu, T.Yamano, L.Gao, F.Zhang, R.Ishitani, O.Nureki. Structural Basis For the Altered Pam Recognition By Engineered Crispr-CPF1 Mol. Cell V. 67 139 2017.
ISSN: ISSN 1097-4164
PubMed: 28595896
DOI: 10.1016/J.MOLCEL.2017.04.019