Chlorine in PDB 6bn0: Avirulence Protein 4 (AVR4) From Cladosporium Fulvum Bound to the Hexasaccharide of Chitin

The structure of Avirulence Protein 4 (AVR4) From Cladosporium Fulvum Bound to the Hexasaccharide of Chitin, PDB code: 6bn0 was solved by N.K.Hurlburt, A.J.Fisher, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	60.11 / 1.95
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	39.864, 41.081, 121.365, 90.00, 97.87, 90.00
R / Rfree (%)	16.8 / 21.4

The binding sites of Chlorine atom in the Avirulence Protein 4 (AVR4) From Cladosporium Fulvum Bound to the Hexasaccharide of Chitin (pdb code 6bn0). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Avirulence Protein 4 (AVR4) From Cladosporium Fulvum Bound to the Hexasaccharide of Chitin, PDB code: 6bn0:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Avirulence Protein 4 (AVR4) From Cladosporium Fulvum Bound to the Hexasaccharide of Chitin


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Avirulence Protein 4 (AVR4) From Cladosporium Fulvum Bound to the Hexasaccharide of Chitin within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl207 b:38.6 occ:1.00 N C:CYS64 3.2 30.0 1.0 O A:HOH348 3.2 42.4 1.0 O A:HOH349 3.2 39.2 1.0 N A:GLY89 3.3 27.4 1.0 CG2 C:THR65 3.7 33.6 1.0 CA A:LYS88 3.8 29.6 1.0 N C:THR65 3.8 33.8 1.0 CB C:CYS64 3.9 38.1 1.0 CA C:CYS64 3.9 32.5 1.0 CB C:SER63 4.0 44.1 1.0 CA C:SER63 4.0 31.8 1.0 C C:SER63 4.1 35.7 1.0 C A:LYS88 4.1 32.3 1.0 OG1 C:THR65 4.1 34.9 1.0 CG A:LYS88 4.2 37.1 1.0 CA A:GLY89 4.2 29.2 1.0 C C:CYS64 4.3 29.6 1.0 CB C:THR65 4.5 32.4 1.0 CB A:LYS88 4.5 34.4 1.0 OG C:SER63 4.6 39.0 1.0 SG C:CYS64 4.7 41.0 1.0 O A:PRO87 4.8 31.0 1.0 CA C:THR65 4.8 33.4 1.0 N A:LYS88 4.8 30.1 1.0 CG2 A:THR65 4.8 32.8 1.0

Chlorine binding site 2 out of 2 in the Avirulence Protein 4 (AVR4) From Cladosporium Fulvum Bound to the Hexasaccharide of Chitin


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Avirulence Protein 4 (AVR4) From Cladosporium Fulvum Bound to the Hexasaccharide of Chitin within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl208 b:36.5 occ:1.00 O C:HOH347 3.1 40.9 1.0 N A:CYS64 3.2 36.0 1.0 O C:HOH344 3.2 46.8 1.0 N C:GLY89 3.2 33.8 1.0 CB A:CYS64 3.7 34.0 1.0 N A:THR65 3.8 32.7 1.0 CA C:LYS88 3.9 40.7 1.0 CA A:CYS64 3.9 30.3 1.0 CG2 A:THR65 3.9 32.8 1.0 C C:LYS88 4.0 37.5 1.0 C A:SER63 4.1 35.3 1.0 CA A:SER63 4.1 35.2 1.0 CA C:GLY89 4.1 32.7 1.0 OG1 A:THR65 4.1 34.9 1.0 C A:CYS64 4.2 32.3 1.0 CB A:SER63 4.2 35.5 1.0 CG C:LYS88 4.3 41.9 1.0 OG A:SER63 4.3 36.7 1.0 CB A:THR65 4.5 36.0 1.0 CB C:LYS88 4.6 36.7 1.0 SG A:CYS64 4.7 48.4 1.0 O C:PRO87 4.7 35.1 1.0 CA A:THR65 4.8 32.0 1.0 N C:LYS88 4.9 33.7 1.0 CG2 C:THR65 4.9 33.6 1.0 C C:GLY89 5.0 34.2 1.0

N.K.Hurlburt, L.H.Chen, I.Stergiopoulos, A.J.Fisher. Structure of the Cladosporium Fulvum AVR4 Effector in Complex with (Glcnac)6 Reveals the Ligand-Binding Mechanism and Uncouples Its Intrinsic Function From Recognition By the Cf-4 Resistance Protein. Plos Pathog. V. 14 07263 2018.
ISSN: ESSN 1553-7374
PubMed: 30148881
DOI: 10.1371/JOURNAL.PPAT.1007263