Chlorine in PDB 6do1: Structure of Nanobody-Stabilized Angiotensin II Type 1 Receptor Bound to S1I8

The structure of Structure of Nanobody-Stabilized Angiotensin II Type 1 Receptor Bound to S1I8, PDB code: 6do1 was solved by L.M.Wingler, C.Mcmahon, D.P.Staus, R.J.Lefkowitz, A.C.Kruse, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.62 / 2.90
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	135.682, 227.796, 41.500, 90.00, 90.00, 90.00
R / Rfree (%)	30.5 / 35.9

The binding sites of Chlorine atom in the Structure of Nanobody-Stabilized Angiotensin II Type 1 Receptor Bound to S1I8 (pdb code 6do1). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 5 binding sites of Chlorine where determined in the Structure of Nanobody-Stabilized Angiotensin II Type 1 Receptor Bound to S1I8, PDB code: 6do1:
Jump to Chlorine binding site number: 1; 2; 3; 4; 5;

Chlorine binding site 1 out of 5 in the Structure of Nanobody-Stabilized Angiotensin II Type 1 Receptor Bound to S1I8


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Structure of Nanobody-Stabilized Angiotensin II Type 1 Receptor Bound to S1I8 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1002 b:53.2 occ:1.00 O A:HOH1127 3.3 30.1 1.0 CD A:LYS135 3.7 64.0 1.0 NH2 A:ARG139 4.0 65.6 1.0 CB A:LYS135 4.3 43.3 1.0 NZ A:LYS135 4.6 57.2 1.0 CG A:LYS135 4.6 54.1 1.0 CE A:LYS135 4.7 55.4 1.0

Chlorine binding site 2 out of 5 in the Structure of Nanobody-Stabilized Angiotensin II Type 1 Receptor Bound to S1I8


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Structure of Nanobody-Stabilized Angiotensin II Type 1 Receptor Bound to S1I8 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1003 b:41.7 occ:1.00 NH2 A:ARG93 3.1 51.9 1.0 CZ A:ARG93 4.1 66.8 1.0 O A:HOH1104 4.2 24.8 1.0 NH1 A:ARG93 4.2 74.3 1.0 O A:ASN176 4.4 51.7 1.0 OG1 A:THR178 4.4 71.1 1.0 CE1 A:PHE171 4.7 46.9 1.0

Chlorine binding site 3 out of 5 in the Structure of Nanobody-Stabilized Angiotensin II Type 1 Receptor Bound to S1I8


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Structure of Nanobody-Stabilized Angiotensin II Type 1 Receptor Bound to S1I8 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1004 b:28.1 occ:1.00 N A:PHE408 3.3 59.5 1.0 N C:TYR109 3.4 40.6 1.0 CB A:LYS407 3.6 51.6 1.0 N A:LYS407 3.7 54.4 1.0 CA A:GLY405 3.9 40.9 1.0 CB C:TYR109 4.0 45.8 1.0 CA A:LYS407 4.0 49.3 1.0 CD2 C:TYR109 4.0 36.0 1.0 CB A:PHE408 4.0 36.8 1.0 C A:GLY405 4.0 46.8 1.0 CA C:TYR109 4.0 54.6 1.0 N C:ASP110 4.0 47.1 1.0 C A:LYS407 4.1 57.1 1.0 OD1 C:ASP108 4.2 65.3 1.0 CA A:PHE408 4.2 46.0 1.0 C C:TYR109 4.2 57.5 1.0 C C:ASP108 4.3 43.8 1.0 CA C:ASP108 4.3 55.7 1.0 N A:LYS406 4.3 55.0 1.0 O A:GLY405 4.4 39.9 1.0 CG C:TYR109 4.4 40.2 1.0 CB C:ASP110 4.5 44.1 1.0 SD A:MET57 4.5 61.9 1.0 C A:LYS406 4.7 56.0 1.0 CG C:ASP108 4.8 68.3 1.0 CA C:ASP110 4.9 48.2 1.0

Chlorine binding site 4 out of 5 in the Structure of Nanobody-Stabilized Angiotensin II Type 1 Receptor Bound to S1I8


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Structure of Nanobody-Stabilized Angiotensin II Type 1 Receptor Bound to S1I8 within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl1002 b:40.1 occ:1.00 NH1 B:ARG410 2.9 63.3 1.0 OH B:TYR411 3.5 59.9 1.0 CZ B:TYR411 3.6 60.9 1.0 CE1 B:TYR411 4.0 56.4 1.0 CE2 B:TYR411 4.2 57.4 1.0 CZ B:ARG410 4.3 78.5 1.0 CD1 B:TYR411 4.8 54.5 1.0 CD B:ARG410 4.9 75.5 1.0 CD2 B:TYR411 4.9 58.7 1.0

Chlorine binding site 5 out of 5 in the Structure of Nanobody-Stabilized Angiotensin II Type 1 Receptor Bound to S1I8


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 5 of Structure of Nanobody-Stabilized Angiotensin II Type 1 Receptor Bound to S1I8 within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl1003 b:42.3 occ:1.00 N B:PHE408 3.0 56.6 1.0 N B:LYS407 3.4 44.3 1.0 CA B:GLY405 3.5 46.2 1.0 CB B:LYS407 3.5 43.5 1.0 C B:GLY405 3.5 54.9 1.0 CB B:PHE408 3.7 47.1 1.0 CA B:LYS407 3.7 48.0 1.0 C B:LYS407 3.8 55.4 1.0 N B:LYS406 3.9 43.6 1.0 CA B:PHE408 3.9 50.2 1.0 O B:GLY405 4.0 53.9 1.0 N D:TYR109 4.0 53.5 1.0 C B:LYS406 4.3 53.9 1.0 N D:ASP110 4.4 45.2 1.0 CB D:ASP110 4.4 45.5 1.0 OD1 D:ASP108 4.5 52.2 1.0 SD B:MET57 4.5 50.0 1.0 CD2 D:TYR109 4.5 45.5 1.0 CB D:TYR109 4.6 28.4 1.0 CA D:TYR109 4.6 51.5 1.0 C D:TYR109 4.7 48.6 1.0 CA B:LYS406 4.7 46.0 1.0 N B:GLY405 4.7 41.1 1.0 CA D:ASP108 4.9 55.5 1.0 C D:ASP108 4.9 46.5 1.0 O B:LYS407 4.9 56.1 1.0 CE B:MET57 4.9 38.9 1.0

L.M.Wingler, C.Mcmahon, D.P.Staus, R.J.Lefkowitz, A.C.Kruse. Distinctive Activation Mechanism For Angiotensin Receptor Revealed By A Synthetic Nanobody. Cell V. 176 479 2019.
ISSN: ISSN 1097-4172
PubMed: 30639100
DOI: 10.1016/J.CELL.2018.12.006