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Chlorine in PDB 6dov: Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Rb+ For 80 S at 21 C

Enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Rb+ For 80 S at 21 C

All present enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Rb+ For 80 S at 21 C:
3.1.26.4;

Protein crystallography data

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Rb+ For 80 S at 21 C, PDB code: 6dov was solved by N.L.Samara, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 20.06 / 1.52
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 81.656, 37.396, 61.670, 90.00, 96.51, 90.00
R / Rfree (%) 15.3 / 19

Other elements in 6dov:

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Rb+ For 80 S at 21 C also contains other interesting chemical elements:

Rubidium (Rb) 5 atoms
Magnesium (Mg) 2 atoms

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Rb+ For 80 S at 21 C (pdb code 6dov). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 3 binding sites of Chlorine where determined in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Rb+ For 80 S at 21 C, PDB code: 6dov:
Jump to Chlorine binding site number: 1; 2; 3;

Chlorine binding site 1 out of 3 in 6dov

Go back to Chlorine Binding Sites List in 6dov
Chlorine binding site 1 out of 3 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Rb+ For 80 S at 21 C


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Rb+ For 80 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl207

b:22.1
occ:0.75
HH A:TYR83 2.3 26.9 1.0
HD22 A:LEU111 2.9 24.8 1.0
HB3 A:LEU111 3.1 20.4 1.0
OH A:TYR83 3.1 22.4 1.0
O A:HOH373 3.2 23.0 1.0
HD22 A:LEU161 3.2 29.7 1.0
HE2 A:TYR83 3.2 25.0 1.0
HD13 A:LEU111 3.2 25.3 1.0
HB3 A:LEU161 3.2 21.6 1.0
O A:HOH380 3.3 28.5 1.0
O A:HOH379 3.3 22.5 1.0
HD13 A:LEU161 3.4 29.7 1.0
C3 A:GOL212 3.6 47.5 1.0
HD3 A:ARG97 3.7 27.9 1.0
CD2 A:LEU111 3.8 20.7 1.0
CE2 A:TYR83 3.9 20.9 1.0
CZ A:TYR83 3.9 18.8 1.0
CB A:LEU111 4.0 17.0 1.0
CD1 A:LEU111 4.0 21.1 1.0
O3 A:GOL212 4.1 31.8 1.0
CD2 A:LEU161 4.1 24.8 1.0
CB A:LEU161 4.1 18.0 1.0
CG A:LEU111 4.1 19.1 1.0
CD1 A:LEU161 4.3 24.7 1.0
HH11 A:ARG97 4.3 32.6 1.0
HD21 A:LEU111 4.3 24.8 1.0
HD23 A:LEU111 4.4 24.8 1.0
CG A:LEU161 4.4 20.2 1.0
HB2 A:HIS115 4.4 21.6 1.0
HB2 A:LEU111 4.5 20.4 1.0
HG2 A:ARG97 4.5 22.3 1.0
HD11 A:LEU111 4.6 25.3 1.0
HB2 A:LEU161 4.6 21.6 1.0
HD23 A:LEU161 4.6 29.7 1.0
O A:HOH417 4.6 24.4 1.0
HD12 A:LEU111 4.7 25.3 1.0
HD21 A:LEU161 4.7 29.7 1.0
CD A:ARG97 4.7 23.3 1.0
HA A:LEU111 4.7 21.3 1.0
O A:LEU111 4.8 16.3 1.0
HD11 A:LEU161 4.8 29.7 1.0
C2 A:GOL212 4.8 65.5 1.0
HD12 A:LEU161 4.9 29.7 1.0
CA A:LEU111 4.9 17.8 1.0
O A:HOH434 4.9 37.3 1.0
NH1 A:ARG97 4.9 27.2 1.0
O A:HOH332 5.0 22.9 1.0

Chlorine binding site 2 out of 3 in 6dov

Go back to Chlorine Binding Sites List in 6dov
Chlorine binding site 2 out of 3 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Rb+ For 80 S at 21 C


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Rb+ For 80 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl208

b:40.6
occ:0.50
O A:HOH443 2.5 47.8 1.0
O A:HOH461 2.7 37.0 1.0
O A:HOH365 2.8 26.2 1.0
HB2 A:LYS180 3.2 27.5 1.0
HA A:GLN134 3.2 29.3 1.0
HD12 A:ILE137 3.3 26.6 1.0
HD3 A:LYS180 3.3 30.4 1.0
HG21 A:ILE137 3.3 25.8 1.0
HD13 A:ILE137 3.4 26.6 1.0
HB A:ILE137 3.5 23.4 1.0
HG3 A:GLN134 3.5 35.0 1.0
CD1 A:ILE137 3.8 22.1 1.0
CG2 A:ILE137 4.0 21.5 1.0
HG22 A:ILE137 4.1 25.8 1.0
CB A:LYS180 4.1 22.9 1.0
CB A:ILE137 4.1 19.5 1.0
CD A:LYS180 4.2 25.3 1.0
CA A:GLN134 4.2 24.4 1.0
O A:HOH308 4.3 37.0 1.0
HG2 A:LYS180 4.3 30.6 1.0
CG A:GLN134 4.4 29.2 1.0
CG A:LYS180 4.4 25.5 1.0
O A:HOH459 4.5 50.5 1.0
HB3 A:LYS180 4.5 27.5 1.0
O A:HOH433 4.6 31.8 1.0
O A:GLN134 4.6 23.1 1.0
CG1 A:ILE137 4.6 20.0 1.0
O A:HOH453 4.6 40.5 1.0
HD11 A:ILE137 4.6 26.6 1.0
HD2 A:LYS180 4.7 30.4 1.0
CB A:GLN134 4.7 24.2 1.0
HG2 A:GLN134 4.7 35.0 1.0
O A:HOH457 4.8 33.4 1.0
HB2 A:GLN134 4.8 29.0 1.0
HG23 A:ILE137 4.8 25.8 1.0
C A:GLN134 4.9 24.2 1.0
HA A:LYS180 4.9 24.0 1.0
HE21 A:GLN134 4.9 44.1 1.0
HE3 A:LYS180 5.0 32.4 1.0

Chlorine binding site 3 out of 3 in 6dov

Go back to Chlorine Binding Sites List in 6dov
Chlorine binding site 3 out of 3 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Rb+ For 80 S at 21 C


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Rb+ For 80 S at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl209

b:37.9
occ:0.75
O A:HOH467 2.7 28.8 0.8
HD11 A:LEU179 3.1 26.5 1.0
HG3 A:PRO128 3.1 26.0 1.0
HB3 A:PRO128 3.3 26.8 1.0
HD21 A:LEU179 3.7 29.5 1.0
CG A:PRO177 3.7 22.2 1.0
CB A:PRO177 3.8 22.2 1.0
CD1 A:ILE64 3.9 37.3 1.0
CG A:PRO128 3.9 21.6 1.0
CB A:PRO128 4.0 22.3 1.0
CD1 A:LEU179 4.0 22.1 1.0
HD3 A:PRO128 4.3 24.5 1.0
O A:HOH468 4.3 52.4 1.0
HB2 A:PRO128 4.4 26.8 1.0
HD13 A:LEU179 4.4 26.5 1.0
HD12 A:LEU179 4.5 26.5 1.0
CD2 A:LEU179 4.5 24.6 1.0
HG2 A:PRO128 4.6 26.0 1.0
HG A:LEU179 4.6 26.6 1.0
CG A:LEU179 4.7 22.2 1.0
CD A:PRO128 4.7 20.4 1.0
CD A:PRO177 4.7 20.3 1.0
HD22 A:LEU179 4.9 29.5 1.0

Reference:

N.L.Samara, W.Yang. Cation Trafficking Propels Rna Hydrolysis. Nat. Struct. Mol. Biol. V. 25 715 2018.
ISSN: ESSN 1545-9985
PubMed: 30076410
DOI: 10.1038/S41594-018-0099-4
Page generated: Sat Jul 27 21:45:42 2024

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