Chlorine in PDB 6dp8: Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Li+ For 240 S at 21 C
Enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Li+ For 240 S at 21 C
All present enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Li+ For 240 S at 21 C:
3.1.26.4;
Protein crystallography data
The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Li+ For 240 S at 21 C, PDB code: 6dp8
was solved by
N.L.Samara,
W.Yang,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
23.46 /
1.32
|
Space group
|
C 1 2 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
81.426,
37.747,
61.811,
90.00,
95.45,
90.00
|
R / Rfree (%)
|
14.7 /
17
|
Other elements in 6dp8:
The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Li+ For 240 S at 21 C also contains other interesting chemical elements:
Chlorine Binding Sites:
The binding sites of Chlorine atom in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Li+ For 240 S at 21 C
(pdb code 6dp8). This binding sites where shown within
5.0 Angstroms radius around Chlorine atom.
In total 3 binding sites of Chlorine where determined in the
Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Li+ For 240 S at 21 C, PDB code: 6dp8:
Jump to Chlorine binding site number:
1;
2;
3;
Chlorine binding site 1 out
of 3 in 6dp8
Go back to
Chlorine Binding Sites List in 6dp8
Chlorine binding site 1 out
of 3 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Li+ For 240 S at 21 C
Mono view
Stereo pair view
|
A full contact list of Chlorine with other atoms in the Cl binding
site number 1 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Li+ For 240 S at 21 C within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Cl203
b:49.7
occ:0.85
|
O3
|
A:GOL212
|
2.1
|
72.9
|
1.0
|
HH
|
A:TYR83
|
2.2
|
31.2
|
1.0
|
C3
|
A:GOL212
|
2.9
|
48.7
|
1.0
|
OH
|
A:TYR83
|
3.0
|
26.0
|
1.0
|
HB3
|
A:LEU111
|
3.1
|
15.5
|
1.0
|
HD13
|
A:LEU111
|
3.1
|
18.1
|
1.0
|
HE2
|
A:TYR83
|
3.2
|
22.5
|
1.0
|
HD22
|
A:LEU161
|
3.2
|
28.6
|
1.0
|
HD22
|
A:LEU111
|
3.2
|
16.6
|
1.0
|
HD13
|
A:LEU161
|
3.3
|
26.9
|
1.0
|
C2
|
A:GOL212
|
3.4
|
41.9
|
1.0
|
HB3
|
A:LEU161
|
3.4
|
18.9
|
1.0
|
HD3
|
A:ARG97
|
3.5
|
25.3
|
1.0
|
O
|
A:HOH304
|
3.5
|
37.2
|
1.0
|
O2
|
A:GOL212
|
3.8
|
84.8
|
1.0
|
CE2
|
A:TYR83
|
3.8
|
18.8
|
1.0
|
CZ
|
A:TYR83
|
3.8
|
21.0
|
1.0
|
HH11
|
A:ARG97
|
4.0
|
33.9
|
1.0
|
O
|
A:HOH306
|
4.0
|
20.5
|
1.0
|
CB
|
A:LEU111
|
4.0
|
12.9
|
1.0
|
CD1
|
A:LEU111
|
4.0
|
15.0
|
1.0
|
CD2
|
A:LEU111
|
4.1
|
13.8
|
1.0
|
CD2
|
A:LEU161
|
4.1
|
23.8
|
1.0
|
CD1
|
A:LEU161
|
4.2
|
22.4
|
1.0
|
CG
|
A:LEU111
|
4.2
|
13.5
|
1.0
|
CB
|
A:LEU161
|
4.3
|
15.8
|
1.0
|
CG
|
A:LEU161
|
4.4
|
19.6
|
1.0
|
HG2
|
A:ARG97
|
4.5
|
21.9
|
1.0
|
HB2
|
A:HIS115
|
4.5
|
15.7
|
1.0
|
CD
|
A:ARG97
|
4.5
|
21.1
|
1.0
|
NH1
|
A:ARG97
|
4.5
|
28.3
|
1.0
|
HB2
|
A:LEU111
|
4.5
|
15.5
|
1.0
|
HD11
|
A:LEU111
|
4.6
|
18.1
|
1.0
|
HD21
|
A:LEU161
|
4.6
|
28.6
|
1.0
|
HD12
|
A:LEU111
|
4.6
|
18.1
|
1.0
|
HD11
|
A:LEU161
|
4.6
|
26.9
|
1.0
|
HD21
|
A:LEU111
|
4.6
|
16.6
|
1.0
|
HD23
|
A:LEU111
|
4.7
|
16.6
|
1.0
|
HD11
|
A:ILE100
|
4.7
|
23.2
|
1.0
|
HD23
|
A:LEU161
|
4.7
|
28.6
|
1.0
|
HD12
|
A:LEU161
|
4.8
|
26.9
|
1.0
|
C1
|
A:GOL212
|
4.8
|
31.2
|
1.0
|
HB2
|
A:LEU161
|
4.8
|
18.9
|
1.0
|
HA
|
A:LEU111
|
4.9
|
15.6
|
1.0
|
HH12
|
A:ARG97
|
4.9
|
33.9
|
1.0
|
O
|
A:LEU111
|
4.9
|
13.5
|
1.0
|
O
|
A:HOH367
|
4.9
|
20.6
|
1.0
|
HB2
|
A:ARG97
|
5.0
|
20.2
|
1.0
|
CA
|
A:LEU111
|
5.0
|
13.0
|
1.0
|
CG
|
A:ARG97
|
5.0
|
18.3
|
1.0
|
|
Chlorine binding site 2 out
of 3 in 6dp8
Go back to
Chlorine Binding Sites List in 6dp8
Chlorine binding site 2 out
of 3 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Li+ For 240 S at 21 C
Mono view
Stereo pair view
|
A full contact list of Chlorine with other atoms in the Cl binding
site number 2 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Li+ For 240 S at 21 C within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Cl204
b:32.3
occ:0.60
|
O
|
A:HOH365
|
3.0
|
20.3
|
1.0
|
HA
|
A:GLN134
|
3.0
|
20.1
|
1.0
|
HD3
|
A:LYS180
|
3.1
|
21.1
|
1.0
|
O
|
A:HOH462
|
3.1
|
47.2
|
1.0
|
HB2
|
A:LYS180
|
3.2
|
22.1
|
1.0
|
HG3
|
A:GLN134
|
3.2
|
23.9
|
1.0
|
O
|
A:HOH346
|
3.3
|
25.3
|
1.0
|
HD12
|
A:ILE137
|
3.4
|
19.9
|
1.0
|
HD13
|
A:ILE137
|
3.7
|
19.9
|
1.0
|
HG2
|
A:LYS180
|
3.8
|
21.7
|
1.0
|
HG21
|
A:ILE137
|
3.8
|
20.9
|
1.0
|
HB
|
A:ILE137
|
3.9
|
17.4
|
1.0
|
CD
|
A:LYS180
|
3.9
|
17.6
|
1.0
|
CA
|
A:GLN134
|
3.9
|
16.8
|
1.0
|
CB
|
A:LYS180
|
4.0
|
18.4
|
1.0
|
CD1
|
A:ILE137
|
4.0
|
16.5
|
1.0
|
CG
|
A:LYS180
|
4.1
|
18.1
|
1.0
|
CG
|
A:GLN134
|
4.1
|
19.9
|
1.0
|
HB2
|
A:GLN134
|
4.1
|
20.0
|
1.0
|
CB
|
A:GLN134
|
4.3
|
16.7
|
1.0
|
HE21
|
A:GLN134
|
4.3
|
23.8
|
1.0
|
HB3
|
A:LYS180
|
4.4
|
22.1
|
1.0
|
HE3
|
A:LYS180
|
4.4
|
22.2
|
1.0
|
CG2
|
A:ILE137
|
4.4
|
17.4
|
1.0
|
HG22
|
A:ILE137
|
4.5
|
20.9
|
1.0
|
CB
|
A:ILE137
|
4.5
|
14.5
|
1.0
|
HG2
|
A:GLN134
|
4.6
|
23.9
|
1.0
|
O
|
A:GLN134
|
4.6
|
16.8
|
1.0
|
HD2
|
A:LYS180
|
4.6
|
21.1
|
1.0
|
O
|
A:HOH463
|
4.6
|
53.8
|
1.0
|
O
|
A:HOH454
|
4.6
|
27.8
|
1.0
|
CE
|
A:LYS180
|
4.7
|
18.5
|
1.0
|
HD11
|
A:ILE137
|
4.7
|
19.9
|
1.0
|
C
|
A:GLN134
|
4.8
|
15.5
|
1.0
|
N
|
A:GLN134
|
4.8
|
15.8
|
1.0
|
HE2
|
A:LYS180
|
4.9
|
22.2
|
1.0
|
CG1
|
A:ILE137
|
4.9
|
14.1
|
1.0
|
O
|
A:HOH433
|
5.0
|
25.7
|
1.0
|
NE2
|
A:GLN134
|
5.0
|
19.8
|
1.0
|
|
Chlorine binding site 3 out
of 3 in 6dp8
Go back to
Chlorine Binding Sites List in 6dp8
Chlorine binding site 3 out
of 3 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Li+ For 240 S at 21 C
Mono view
Stereo pair view
|
A full contact list of Chlorine with other atoms in the Cl binding
site number 3 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Reaction in 5 Mm MG2+ and 200 Mm Li+ For 240 S at 21 C within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Cl205
b:50.2
occ:0.85
|
O
|
A:HOH464
|
2.5
|
43.6
|
1.0
|
HG2
|
A:PRO177
|
2.9
|
20.6
|
1.0
|
HD11
|
A:LEU179
|
3.0
|
25.3
|
1.0
|
HD13
|
A:ILE64
|
3.0
|
40.7
|
1.0
|
HG3
|
A:PRO128
|
3.1
|
24.0
|
1.0
|
HB3
|
A:PRO128
|
3.4
|
21.7
|
1.0
|
HB2
|
A:PRO177
|
3.4
|
21.1
|
1.0
|
HD21
|
A:LEU179
|
3.6
|
26.3
|
1.0
|
CD1
|
A:ILE64
|
3.6
|
33.9
|
1.0
|
HD12
|
A:ILE64
|
3.7
|
40.7
|
1.0
|
HD11
|
A:ILE64
|
3.7
|
40.7
|
1.0
|
CG
|
A:PRO177
|
3.7
|
17.2
|
1.0
|
CB
|
A:PRO177
|
3.8
|
17.5
|
1.0
|
HB3
|
A:PRO177
|
3.8
|
21.1
|
1.0
|
CD1
|
A:LEU179
|
3.9
|
21.1
|
1.0
|
CG
|
A:PRO128
|
4.0
|
20.0
|
1.0
|
CB
|
A:PRO128
|
4.1
|
18.1
|
1.0
|
HG3
|
A:PRO177
|
4.2
|
20.6
|
1.0
|
HD13
|
A:LEU179
|
4.3
|
25.3
|
1.0
|
HG21
|
A:ILE64
|
4.3
|
26.8
|
1.0
|
HB2
|
A:PRO128
|
4.4
|
21.7
|
1.0
|
CD2
|
A:LEU179
|
4.4
|
21.9
|
1.0
|
HD3
|
A:PRO128
|
4.5
|
20.0
|
1.0
|
HD12
|
A:LEU179
|
4.5
|
25.3
|
1.0
|
HG2
|
A:PRO128
|
4.6
|
24.0
|
1.0
|
CG
|
A:LEU179
|
4.6
|
19.0
|
1.0
|
HG
|
A:LEU179
|
4.7
|
22.8
|
1.0
|
HD2
|
A:PRO177
|
4.7
|
18.9
|
1.0
|
HD22
|
A:LEU179
|
4.7
|
26.3
|
1.0
|
CD
|
A:PRO128
|
4.8
|
16.6
|
1.0
|
CD
|
A:PRO177
|
4.8
|
15.7
|
1.0
|
O
|
A:HOH436
|
5.0
|
48.6
|
1.0
|
|
Reference:
N.L.Samara,
W.Yang.
Cation Trafficking Propels Rna Hydrolysis. Nat. Struct. Mol. Biol. V. 25 715 2018.
ISSN: ESSN 1545-9985
PubMed: 30076410
DOI: 10.1038/S41594-018-0099-4
Page generated: Sat Jul 27 21:45:42 2024
|