Chlorine in PDB 6f0j: Glic Mutant E26A

The structure of Glic Mutant E26A, PDB code: 6f0j was solved by H.D.Hu, M.Delarue, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	25.00 / 3.15
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	181.010, 133.110, 159.850, 90.00, 102.14, 90.00
R / Rfree (%)	20.8 / 22.2

The structure of Glic Mutant E26A also contains other interesting chemical elements:

Sodium

(Na)

6 atoms

The binding sites of Chlorine atom in the Glic Mutant E26A (pdb code 6f0j). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 7 binding sites of Chlorine where determined in the Glic Mutant E26A, PDB code: 6f0j:
Jump to Chlorine binding site number: 1; 2; 3; 4; 5; 6; 7;

Chlorine binding site 1 out of 7 in the Glic Mutant E26A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Glic Mutant E26A within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl402 b:83.0 occ:1.00 N A:PHE78 3.2 86.7 1.0 CD1 A:PHE78 3.7 94.9 1.0 CG2 A:VAL81 3.8 88.5 1.0 CB A:PHE78 3.8 88.2 1.0 N A:ARG85 3.8 0.3 1.0 NH1 A:ARG85 3.9 0.8 1.0 O A:PHE78 3.9 89.0 1.0 CA A:PHE78 4.0 86.4 1.0 CA A:ARG77 4.0 81.7 1.0 C A:ARG77 4.1 89.5 1.0 CG A:ARG85 4.1 0.6 1.0 CA A:ALA84 4.1 0.8 1.0 CB A:ARG77 4.2 81.0 1.0 CG A:PHE78 4.3 91.2 1.0 CB A:ARG85 4.4 97.4 1.0 C A:PHE78 4.4 88.1 1.0 C A:ALA84 4.5 0.4 1.0 CB A:ALA84 4.7 0.3 1.0 CA A:ARG85 4.7 0.1 1.0 CG1 A:VAL81 4.8 88.5 1.0 CG A:ARG77 4.8 92.1 1.0 CB A:VAL81 4.9 88.2 1.0 CE1 A:PHE78 4.9 96.4 1.0 CD A:ARG85 4.9 0.3 1.0

Chlorine binding site 2 out of 7 in the Glic Mutant E26A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Glic Mutant E26A within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl403 b:0.7 occ:1.00 N A:LEU180 3.5 90.8 1.0 OD1 A:ASN139 3.6 0.9 1.0 ND2 A:ASN139 3.8 0.5 1.0 CA A:ARG179 3.9 89.9 1.0 CG A:ASN139 4.0 0.7 1.0 CB A:LEU180 4.0 91.7 1.0 C A:ARG179 4.2 93.3 1.0 O A:ASP178 4.3 98.5 1.0 CA A:LEU180 4.4 90.5 1.0 CD A:ARG179 4.6 0.1 1.0 CB A:SER134 4.6 94.1 1.0 CB A:ARG179 4.7 86.7 1.0 CG A:ARG179 4.9 92.6 1.0 N A:ARG179 4.9 91.3 1.0 C A:ASP178 5.0 97.3 1.0

Chlorine binding site 3 out of 7 in the Glic Mutant E26A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Glic Mutant E26A within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl404 b:0.1 occ:1.00 N A:ALA175 3.3 88.0 1.0 CB A:ALA175 3.8 90.2 1.0 CA A:PHE174 4.0 82.8 1.0 CA A:ALA175 4.1 88.2 1.0 C A:PHE174 4.2 89.1 1.0 CB A:PHE174 4.3 82.3 1.0 O A:ALA175 4.4 92.0 1.0 C A:ALA175 4.7 92.8 1.0

Chlorine binding site 4 out of 7 in the Glic Mutant E26A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Glic Mutant E26A within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl403 b:0.7 occ:1.00 N B:PHE78 3.0 79.7 1.0 CD1 B:PHE78 3.6 88.1 1.0 CB B:PHE78 3.6 81.0 1.0 CG2 B:VAL81 3.7 87.2 1.0 O B:PHE78 3.8 86.1 1.0 CA B:PHE78 3.8 79.5 1.0 NH1 B:ARG85 3.8 95.1 1.0 CA B:ARG77 3.9 79.6 1.0 C B:ARG77 3.9 82.4 1.0 N B:ARG85 4.0 99.4 1.0 CG B:PHE78 4.1 83.3 1.0 CG B:ARG85 4.1 97.7 1.0 CB B:ARG77 4.1 80.3 1.0 C B:PHE78 4.3 84.4 1.0 CA B:ALA84 4.4 0.6 1.0 CB B:ARG85 4.5 93.6 1.0 CG1 B:VAL81 4.7 85.0 1.0 CE1 B:PHE78 4.7 89.1 1.0 C B:ALA84 4.8 0.5 1.0 CB B:VAL81 4.8 86.5 1.0 CG B:ARG77 4.8 91.1 1.0 CD B:ARG85 4.9 0.5 1.0 CA B:ARG85 4.9 98.1 1.0 CZ B:ARG85 5.0 0.0 1.0 CB B:ALA84 5.0 0.7 1.0

Chlorine binding site 5 out of 7 in the Glic Mutant E26A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 5 of Glic Mutant E26A within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl402 b:92.0 occ:1.00 N C:PHE78 3.0 76.6 1.0 CB C:PHE78 3.7 77.0 1.0 CD1 C:PHE78 3.7 82.3 1.0 O C:PHE78 3.8 83.6 1.0 CA C:ARG77 3.8 74.5 1.0 CA C:PHE78 3.8 75.9 1.0 C C:ARG77 3.9 79.8 1.0 CG2 C:VAL81 3.9 93.7 1.0 NH1 C:ARG85 3.9 93.1 1.0 CB C:ARG77 4.0 74.7 1.0 N C:ARG85 4.1 0.2 1.0 CG C:PHE78 4.2 79.0 1.0 CG C:ARG85 4.2 1.0 1.0 C C:PHE78 4.3 81.2 1.0 CA C:ALA84 4.3 0.1 1.0 CB C:ARG85 4.6 0.1 1.0 CG C:ARG77 4.6 94.0 1.0 NH1 B:ARG105 4.7 0.6 1.0 C C:ALA84 4.8 0.4 1.0 CG1 C:VAL81 4.8 91.9 1.0 CB C:ALA84 4.8 0.2 1.0 CE1 C:PHE78 4.9 82.9 1.0 CB C:VAL81 5.0 92.8 1.0 CD C:ARG85 5.0 0.5 1.0

Chlorine binding site 6 out of 7 in the Glic Mutant E26A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 6 of Glic Mutant E26A within 5.0Å range: probe atom residue distance (Å) B Occ D:Cl401 b:91.8 occ:1.00 N D:PHE78 3.2 76.4 1.0 CG2 D:VAL81 3.7 85.7 1.0 N D:ARG85 3.8 0.1 1.0 CD1 D:PHE78 3.8 85.3 1.0 CB D:PHE78 3.8 78.2 1.0 O D:PHE78 3.9 81.1 1.0 NH1 D:ARG85 4.0 91.5 1.0 CA D:PHE78 4.0 76.1 1.0 CA D:ARG77 4.0 75.2 1.0 CA D:ALA84 4.1 0.0 1.0 CG D:ARG85 4.1 99.0 1.0 C D:ARG77 4.1 79.7 1.0 CB D:ARG77 4.2 76.2 1.0 CG D:PHE78 4.3 81.0 1.0 CB D:ARG85 4.4 97.0 1.0 C D:PHE78 4.5 79.6 1.0 C D:ALA84 4.5 0.3 1.0 CB D:ALA84 4.6 0.0 1.0 CA D:ARG85 4.7 99.6 1.0 CG1 D:VAL81 4.7 84.7 1.0 CB D:VAL81 4.8 85.2 1.0 CG D:ARG77 4.8 89.1 1.0 CD D:ARG85 4.9 0.5 1.0 CE1 D:PHE78 4.9 86.6 1.0

Chlorine binding site 7 out of 7 in the Glic Mutant E26A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 7 of Glic Mutant E26A within 5.0Å range: probe atom residue distance (Å) B Occ E:Cl402 b:90.1 occ:1.00 N E:PHE78 3.1 78.1 1.0 CG2 E:VAL81 3.7 89.8 1.0 CB E:PHE78 3.7 78.9 1.0 O E:PHE78 3.7 83.2 1.0 CD1 E:PHE78 3.9 84.2 1.0 CA E:PHE78 3.9 77.6 1.0 CA E:ARG77 3.9 78.3 1.0 C E:ARG77 4.0 82.1 1.0 CB E:ARG77 4.0 78.3 1.0 NH1 E:ARG85 4.1 0.7 1.0 N E:ARG85 4.1 0.9 1.0 CA E:ALA84 4.2 0.2 1.0 C E:PHE78 4.3 82.3 1.0 CG E:PHE78 4.3 80.8 1.0 CG E:ARG85 4.4 1.0 1.0 CG E:ARG77 4.7 93.6 1.0 CB E:ARG85 4.7 0.5 1.0 C E:ALA84 4.7 0.8 1.0 CB E:ALA84 4.8 0.3 1.0 CG1 E:VAL81 4.8 88.8 1.0 CB E:VAL81 4.9 89.3 1.0

A.Nemecz, H.Hu, Z.Fourati, C.Van Renterghem, M.Delarue, P.J.Corringer. Full Mutational Mapping of Titratable Residues Helps to Identify Proton-Sensors Involved in the Control of Channel Gating in the Gloeobacter Violaceus Pentameric Ligand-Gated Ion Channel. Plos Biol. V. 15 04470 2017.
ISSN: ESSN 1545-7885
PubMed: 29281623
DOI: 10.1371/JOURNAL.PBIO.2004470