Chlorine in PDB 6hpf: Structure of Inactive E165Q Mutant of Fungal Non-Cbm Carrying GH26 Endo-B-Mannanase From Yunnania Penicillata in Complex with Alpha-62- 61-Di-Galactosyl-Mannotriose

The structure of Structure of Inactive E165Q Mutant of Fungal Non-Cbm Carrying GH26 Endo-B-Mannanase From Yunnania Penicillata in Complex with Alpha-62- 61-Di-Galactosyl-Mannotriose, PDB code: 6hpf was solved by P.Von Freiesleben, O.V.Moroz, E.Blagova, M.Wiemann, N.Spodsberg, J.W.Agger, G.J.Davies, K.S.Wilson, H.Stalbrand, A.S.Meyer, K.B.R.M.Krogh, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	34.24 / 1.36
Space group	P 65 2 2
Cell size a, b, c (Å), α, β, γ (°)	98.988, 98.988, 170.497, 90.00, 90.00, 120.00
R / Rfree (%)	12.2 / 14.3

The binding sites of Chlorine atom in the Structure of Inactive E165Q Mutant of Fungal Non-Cbm Carrying GH26 Endo-B-Mannanase From Yunnania Penicillata in Complex with Alpha-62- 61-Di-Galactosyl-Mannotriose (pdb code 6hpf). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the Structure of Inactive E165Q Mutant of Fungal Non-Cbm Carrying GH26 Endo-B-Mannanase From Yunnania Penicillata in Complex with Alpha-62- 61-Di-Galactosyl-Mannotriose, PDB code: 6hpf:

Chlorine binding site 1 out of 1 in the Structure of Inactive E165Q Mutant of Fungal Non-Cbm Carrying GH26 Endo-B-Mannanase From Yunnania Penicillata in Complex with Alpha-62- 61-Di-Galactosyl-Mannotriose


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Structure of Inactive E165Q Mutant of Fungal Non-Cbm Carrying GH26 Endo-B-Mannanase From Yunnania Penicillata in Complex with Alpha-62- 61-Di-Galactosyl-Mannotriose within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl514 b:46.2 occ:1.00 N A:ASP13 3.4 19.9 1.0 O A:HOH613 3.5 26.5 1.0 CB A:THR12 3.9 18.1 1.0 CA A:THR12 3.9 18.5 1.0 CG A:ASP13 4.0 33.7 1.0 CB A:ASP13 4.1 25.1 1.0 C A:THR12 4.2 19.6 1.0 OD2 A:ASP13 4.3 36.2 1.0 OD1 A:ASP13 4.3 32.4 1.0 CA A:ASP13 4.4 21.2 1.0 CG2 A:THR12 4.4 20.1 1.0 O A:HOH746 4.5 33.2 1.0

P.Von Freiesleben, O.V.Moroz, E.Blagova, M.Wiemann, N.Spodsberg, J.W.Agger, G.J.Davies, K.S.Wilson, H.Stalbrand, A.S.Meyer, K.B.R.M.Krogh. Crystal Structure and Substrate Interactions of An Unusual Fungal Non-Cbm Carrying GH26 Endo-Beta-Mannanase From Yunnania Penicillata. Sci Rep V. 9 2266 2019.
ISSN: ESSN 2045-2322
PubMed: 30783168
DOI: 10.1038/S41598-019-38602-X