Chlorine in PDB 6hq6: Bacterial Beta-1,3-Oligosaccharide Phosphorylase From GH149

The structure of Bacterial Beta-1,3-Oligosaccharide Phosphorylase From GH149, PDB code: 6hq6 was solved by S.Kuhaudomlarp, C.E.M.Stevenson, D.M.Lawson, R.A.Field, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	79.49 / 2.05
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	100.239, 158.986, 181.564, 90.00, 90.00, 90.00
R / Rfree (%)	18.2 / 21.1

The binding sites of Chlorine atom in the Bacterial Beta-1,3-Oligosaccharide Phosphorylase From GH149 (pdb code 6hq6). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 5 binding sites of Chlorine where determined in the Bacterial Beta-1,3-Oligosaccharide Phosphorylase From GH149, PDB code: 6hq6:
Jump to Chlorine binding site number: 1; 2; 3; 4; 5;

Chlorine binding site 1 out of 5 in the Bacterial Beta-1,3-Oligosaccharide Phosphorylase From GH149


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Bacterial Beta-1,3-Oligosaccharide Phosphorylase From GH149 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1216 b:78.8 occ:1.00 O A:HOH1974 3.0 67.5 1.0 NH2 A:ARG507 3.5 62.2 1.0 N A:LYS448 3.9 43.3 1.0 NH2 A:ARG438 4.1 81.6 1.0 CB A:LYS448 4.3 46.6 1.0 CA A:ASN447 4.5 42.9 1.0 O A:TRP446 4.5 48.8 1.0 CA A:LYS448 4.7 46.5 1.0 O A:LYS448 4.7 54.6 1.0 CZ A:ARG507 4.7 65.5 1.0 C A:ASN447 4.7 43.3 1.0 CZ A:ARG438 4.8 81.0 1.0 NE A:ARG438 4.9 77.8 1.0 OD1 A:ASN447 5.0 44.6 1.0

Chlorine binding site 2 out of 5 in the Bacterial Beta-1,3-Oligosaccharide Phosphorylase From GH149


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Bacterial Beta-1,3-Oligosaccharide Phosphorylase From GH149 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1217 b:0.8 occ:1.00 N A:ASN468 3.5 36.2 1.0 OD2 A:ASP471 3.5 50.3 1.0 CB A:ASP471 3.6 41.1 1.0 CD A:ARG438 3.6 74.6 1.0 O A:HOH1523 3.7 49.0 1.0 CA A:GLY467 3.7 41.6 1.0 NE A:ARG438 4.0 77.8 1.0 CG A:ASP471 4.0 42.9 1.0 CE1 A:HIS439 4.0 58.2 1.0 ND1 A:HIS439 4.1 56.3 1.0 NH1 A:ARG438 4.1 87.2 1.0 C A:GLY467 4.1 38.9 1.0 CB A:ASN468 4.3 38.9 1.0 CG A:ARG367 4.4 35.6 1.0 CA A:ASN468 4.4 38.2 1.0 CZ A:ARG438 4.4 81.0 1.0 O A:ASN468 4.6 36.0 1.0 CD A:ARG367 4.7 39.3 1.0 CG A:ASN468 4.7 43.1 1.0 CG A:ARG438 4.9 69.1 1.0 O A:GLU466 4.9 51.1 1.0 CA A:ASP471 5.0 38.3 1.0

Chlorine binding site 3 out of 5 in the Bacterial Beta-1,3-Oligosaccharide Phosphorylase From GH149


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Bacterial Beta-1,3-Oligosaccharide Phosphorylase From GH149 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1218 b:48.4 occ:0.50 CL A:CL1218 0.0 48.4 0.5 CL A:CL1218 2.5 67.5 0.5 O A:HOH1662 3.0 50.4 1.0 N A:HIS618 3.1 43.1 1.0 ND1 A:HIS618 3.5 44.0 1.0 CB A:HIS618 3.7 43.5 1.0 CD1 A:TYR617 3.8 43.5 1.0 O A:ILE616 3.8 48.4 1.0 CA A:HIS618 3.9 43.0 1.0 CA A:TYR617 3.9 43.9 1.0 CG A:LYS614 4.0 66.1 1.0 O A:HIS618 4.0 43.0 1.0 C A:TYR617 4.0 43.1 1.0 CG A:HIS618 4.1 43.0 1.0 CE1 A:TYR617 4.3 43.0 1.0 C A:HIS618 4.4 42.9 1.0 O A:HOH1556 4.6 49.9 1.0 CB A:LYS614 4.7 62.6 1.0 CE1 A:HIS618 4.7 43.5 1.0 CG A:TYR617 4.7 42.6 1.0 NZ A:LYS750 4.7 49.5 1.0 C A:ILE616 4.7 47.1 1.0 CE A:LYS614 4.8 74.2 1.0 N A:TYR617 4.8 44.8 1.0 CB A:TYR617 4.9 43.1 1.0 CD A:LYS614 4.9 71.5 1.0

Chlorine binding site 4 out of 5 in the Bacterial Beta-1,3-Oligosaccharide Phosphorylase From GH149


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Bacterial Beta-1,3-Oligosaccharide Phosphorylase From GH149 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1218 b:67.5 occ:0.50 CL A:CL1218 0.0 67.5 0.5 CL A:CL1218 2.5 48.4 0.5 ND1 A:HIS618 2.8 44.0 1.0 CG A:LYS614 3.4 66.1 1.0 CE1 A:HIS618 3.5 43.5 1.0 CE A:LYS614 3.6 74.2 1.0 CD A:LYS614 3.7 71.5 1.0 O A:ILE616 3.7 48.4 1.0 CG A:HIS618 3.9 43.0 1.0 CB A:LYS614 4.1 62.6 1.0 CB A:HIS618 4.4 43.5 1.0 O A:HOH1662 4.5 50.4 1.0 O A:HOH1482 4.7 48.2 1.0 N A:HIS618 4.7 43.1 1.0 NE2 A:HIS618 4.7 41.3 1.0 NZ A:LYS614 4.8 76.3 1.0 C A:ILE616 4.9 47.1 1.0 CD2 A:HIS618 5.0 41.3 1.0

Chlorine binding site 5 out of 5 in the Bacterial Beta-1,3-Oligosaccharide Phosphorylase From GH149


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 5 of Bacterial Beta-1,3-Oligosaccharide Phosphorylase From GH149 within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl1209 b:92.7 occ:1.00 NH1 B:ARG438 3.2 81.2 1.0 N B:LYS448 3.6 58.7 1.0 CA B:ASN447 4.2 58.8 1.0 CB B:LYS448 4.2 61.1 1.0 CZ B:ARG438 4.2 80.6 1.0 O B:TRP446 4.3 64.0 1.0 C B:ASN447 4.4 58.0 1.0 CA B:LYS448 4.4 60.2 1.0 O B:LYS448 4.5 70.4 1.0 NH2 B:ARG438 4.7 79.0 1.0 CB B:ASN447 4.9 59.3 1.0 C B:LYS448 4.9 62.3 1.0 OD1 B:ASN447 5.0 64.9 1.0

S.Kuhaudomlarp, C.E.M.Stevenson, D.M.Lawson, R.A.Field. The Structure of A GH149 Beta-(1 → 3) Glucan Phosphorylase Reveals A New Surface Oligosaccharide Binding Site and Additional Domains That Are Absent in the Disaccharide-Specific GH94 Glucose-Beta-(1 → 3)-Glucose (Laminaribiose) Phosphorylase. Proteins V. 87 885 2019.
ISSN: ESSN 1097-0134
PubMed: 31134667
DOI: 10.1002/PROT.25745