Chlorine in PDB 6hq6: Bacterial Beta-1,3-Oligosaccharide Phosphorylase From GH149
Protein crystallography data
The structure of Bacterial Beta-1,3-Oligosaccharide Phosphorylase From GH149, PDB code: 6hq6
was solved by
S.Kuhaudomlarp,
C.E.M.Stevenson,
D.M.Lawson,
R.A.Field,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
79.49 /
2.05
|
Space group
|
P 21 21 21
|
Cell size a, b, c (Å), α, β, γ (°)
|
100.239,
158.986,
181.564,
90.00,
90.00,
90.00
|
R / Rfree (%)
|
18.2 /
21.1
|
Chlorine Binding Sites:
The binding sites of Chlorine atom in the Bacterial Beta-1,3-Oligosaccharide Phosphorylase From GH149
(pdb code 6hq6). This binding sites where shown within
5.0 Angstroms radius around Chlorine atom.
In total 5 binding sites of Chlorine where determined in the
Bacterial Beta-1,3-Oligosaccharide Phosphorylase From GH149, PDB code: 6hq6:
Jump to Chlorine binding site number:
1;
2;
3;
4;
5;
Chlorine binding site 1 out
of 5 in 6hq6
Go back to
Chlorine Binding Sites List in 6hq6
Chlorine binding site 1 out
of 5 in the Bacterial Beta-1,3-Oligosaccharide Phosphorylase From GH149
Mono view
Stereo pair view
|
A full contact list of Chlorine with other atoms in the Cl binding
site number 1 of Bacterial Beta-1,3-Oligosaccharide Phosphorylase From GH149 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Cl1216
b:78.8
occ:1.00
|
O
|
A:HOH1974
|
3.0
|
67.5
|
1.0
|
NH2
|
A:ARG507
|
3.5
|
62.2
|
1.0
|
N
|
A:LYS448
|
3.9
|
43.3
|
1.0
|
NH2
|
A:ARG438
|
4.1
|
81.6
|
1.0
|
CB
|
A:LYS448
|
4.3
|
46.6
|
1.0
|
CA
|
A:ASN447
|
4.5
|
42.9
|
1.0
|
O
|
A:TRP446
|
4.5
|
48.8
|
1.0
|
CA
|
A:LYS448
|
4.7
|
46.5
|
1.0
|
O
|
A:LYS448
|
4.7
|
54.6
|
1.0
|
CZ
|
A:ARG507
|
4.7
|
65.5
|
1.0
|
C
|
A:ASN447
|
4.7
|
43.3
|
1.0
|
CZ
|
A:ARG438
|
4.8
|
81.0
|
1.0
|
NE
|
A:ARG438
|
4.9
|
77.8
|
1.0
|
OD1
|
A:ASN447
|
5.0
|
44.6
|
1.0
|
|
Chlorine binding site 2 out
of 5 in 6hq6
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Chlorine Binding Sites List in 6hq6
Chlorine binding site 2 out
of 5 in the Bacterial Beta-1,3-Oligosaccharide Phosphorylase From GH149
Mono view
Stereo pair view
|
A full contact list of Chlorine with other atoms in the Cl binding
site number 2 of Bacterial Beta-1,3-Oligosaccharide Phosphorylase From GH149 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Cl1217
b:0.8
occ:1.00
|
N
|
A:ASN468
|
3.5
|
36.2
|
1.0
|
OD2
|
A:ASP471
|
3.5
|
50.3
|
1.0
|
CB
|
A:ASP471
|
3.6
|
41.1
|
1.0
|
CD
|
A:ARG438
|
3.6
|
74.6
|
1.0
|
O
|
A:HOH1523
|
3.7
|
49.0
|
1.0
|
CA
|
A:GLY467
|
3.7
|
41.6
|
1.0
|
NE
|
A:ARG438
|
4.0
|
77.8
|
1.0
|
CG
|
A:ASP471
|
4.0
|
42.9
|
1.0
|
CE1
|
A:HIS439
|
4.0
|
58.2
|
1.0
|
ND1
|
A:HIS439
|
4.1
|
56.3
|
1.0
|
NH1
|
A:ARG438
|
4.1
|
87.2
|
1.0
|
C
|
A:GLY467
|
4.1
|
38.9
|
1.0
|
CB
|
A:ASN468
|
4.3
|
38.9
|
1.0
|
CG
|
A:ARG367
|
4.4
|
35.6
|
1.0
|
CA
|
A:ASN468
|
4.4
|
38.2
|
1.0
|
CZ
|
A:ARG438
|
4.4
|
81.0
|
1.0
|
O
|
A:ASN468
|
4.6
|
36.0
|
1.0
|
CD
|
A:ARG367
|
4.7
|
39.3
|
1.0
|
CG
|
A:ASN468
|
4.7
|
43.1
|
1.0
|
CG
|
A:ARG438
|
4.9
|
69.1
|
1.0
|
O
|
A:GLU466
|
4.9
|
51.1
|
1.0
|
CA
|
A:ASP471
|
5.0
|
38.3
|
1.0
|
|
Chlorine binding site 3 out
of 5 in 6hq6
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Chlorine Binding Sites List in 6hq6
Chlorine binding site 3 out
of 5 in the Bacterial Beta-1,3-Oligosaccharide Phosphorylase From GH149
Mono view
Stereo pair view
|
A full contact list of Chlorine with other atoms in the Cl binding
site number 3 of Bacterial Beta-1,3-Oligosaccharide Phosphorylase From GH149 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Cl1218
b:48.4
occ:0.50
|
CL
|
A:CL1218
|
0.0
|
48.4
|
0.5
|
CL
|
A:CL1218
|
2.5
|
67.5
|
0.5
|
O
|
A:HOH1662
|
3.0
|
50.4
|
1.0
|
N
|
A:HIS618
|
3.1
|
43.1
|
1.0
|
ND1
|
A:HIS618
|
3.5
|
44.0
|
1.0
|
CB
|
A:HIS618
|
3.7
|
43.5
|
1.0
|
CD1
|
A:TYR617
|
3.8
|
43.5
|
1.0
|
O
|
A:ILE616
|
3.8
|
48.4
|
1.0
|
CA
|
A:HIS618
|
3.9
|
43.0
|
1.0
|
CA
|
A:TYR617
|
3.9
|
43.9
|
1.0
|
CG
|
A:LYS614
|
4.0
|
66.1
|
1.0
|
O
|
A:HIS618
|
4.0
|
43.0
|
1.0
|
C
|
A:TYR617
|
4.0
|
43.1
|
1.0
|
CG
|
A:HIS618
|
4.1
|
43.0
|
1.0
|
CE1
|
A:TYR617
|
4.3
|
43.0
|
1.0
|
C
|
A:HIS618
|
4.4
|
42.9
|
1.0
|
O
|
A:HOH1556
|
4.6
|
49.9
|
1.0
|
CB
|
A:LYS614
|
4.7
|
62.6
|
1.0
|
CE1
|
A:HIS618
|
4.7
|
43.5
|
1.0
|
CG
|
A:TYR617
|
4.7
|
42.6
|
1.0
|
NZ
|
A:LYS750
|
4.7
|
49.5
|
1.0
|
C
|
A:ILE616
|
4.7
|
47.1
|
1.0
|
CE
|
A:LYS614
|
4.8
|
74.2
|
1.0
|
N
|
A:TYR617
|
4.8
|
44.8
|
1.0
|
CB
|
A:TYR617
|
4.9
|
43.1
|
1.0
|
CD
|
A:LYS614
|
4.9
|
71.5
|
1.0
|
|
Chlorine binding site 4 out
of 5 in 6hq6
Go back to
Chlorine Binding Sites List in 6hq6
Chlorine binding site 4 out
of 5 in the Bacterial Beta-1,3-Oligosaccharide Phosphorylase From GH149
Mono view
Stereo pair view
|
A full contact list of Chlorine with other atoms in the Cl binding
site number 4 of Bacterial Beta-1,3-Oligosaccharide Phosphorylase From GH149 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Cl1218
b:67.5
occ:0.50
|
CL
|
A:CL1218
|
0.0
|
67.5
|
0.5
|
CL
|
A:CL1218
|
2.5
|
48.4
|
0.5
|
ND1
|
A:HIS618
|
2.8
|
44.0
|
1.0
|
CG
|
A:LYS614
|
3.4
|
66.1
|
1.0
|
CE1
|
A:HIS618
|
3.5
|
43.5
|
1.0
|
CE
|
A:LYS614
|
3.6
|
74.2
|
1.0
|
CD
|
A:LYS614
|
3.7
|
71.5
|
1.0
|
O
|
A:ILE616
|
3.7
|
48.4
|
1.0
|
CG
|
A:HIS618
|
3.9
|
43.0
|
1.0
|
CB
|
A:LYS614
|
4.1
|
62.6
|
1.0
|
CB
|
A:HIS618
|
4.4
|
43.5
|
1.0
|
O
|
A:HOH1662
|
4.5
|
50.4
|
1.0
|
O
|
A:HOH1482
|
4.7
|
48.2
|
1.0
|
N
|
A:HIS618
|
4.7
|
43.1
|
1.0
|
NE2
|
A:HIS618
|
4.7
|
41.3
|
1.0
|
NZ
|
A:LYS614
|
4.8
|
76.3
|
1.0
|
C
|
A:ILE616
|
4.9
|
47.1
|
1.0
|
CD2
|
A:HIS618
|
5.0
|
41.3
|
1.0
|
|
Chlorine binding site 5 out
of 5 in 6hq6
Go back to
Chlorine Binding Sites List in 6hq6
Chlorine binding site 5 out
of 5 in the Bacterial Beta-1,3-Oligosaccharide Phosphorylase From GH149
Mono view
Stereo pair view
|
A full contact list of Chlorine with other atoms in the Cl binding
site number 5 of Bacterial Beta-1,3-Oligosaccharide Phosphorylase From GH149 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Cl1209
b:92.7
occ:1.00
|
NH1
|
B:ARG438
|
3.2
|
81.2
|
1.0
|
N
|
B:LYS448
|
3.6
|
58.7
|
1.0
|
CA
|
B:ASN447
|
4.2
|
58.8
|
1.0
|
CB
|
B:LYS448
|
4.2
|
61.1
|
1.0
|
CZ
|
B:ARG438
|
4.2
|
80.6
|
1.0
|
O
|
B:TRP446
|
4.3
|
64.0
|
1.0
|
C
|
B:ASN447
|
4.4
|
58.0
|
1.0
|
CA
|
B:LYS448
|
4.4
|
60.2
|
1.0
|
O
|
B:LYS448
|
4.5
|
70.4
|
1.0
|
NH2
|
B:ARG438
|
4.7
|
79.0
|
1.0
|
CB
|
B:ASN447
|
4.9
|
59.3
|
1.0
|
C
|
B:LYS448
|
4.9
|
62.3
|
1.0
|
OD1
|
B:ASN447
|
5.0
|
64.9
|
1.0
|
|
Reference:
S.Kuhaudomlarp,
C.E.M.Stevenson,
D.M.Lawson,
R.A.Field.
The Structure of A GH149 Beta-(1 → 3) Glucan Phosphorylase Reveals A New Surface Oligosaccharide Binding Site and Additional Domains That Are Absent in the Disaccharide-Specific GH94 Glucose-Beta-(1 → 3)-Glucose (Laminaribiose) Phosphorylase. Proteins V. 87 885 2019.
ISSN: ESSN 1097-0134
PubMed: 31134667
DOI: 10.1002/PROT.25745
Page generated: Sun Jul 28 01:02:19 2024
|