Chlorine in PDB 6hy5: The Glic Pentameric Ligand-Gated Ion Channel Mutant Q193C

The structure of The Glic Pentameric Ligand-Gated Ion Channel Mutant Q193C, PDB code: 6hy5 was solved by H.D.Hu, M.Delarue, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.58
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	182.420, 133.490, 161.010, 90.00, 102.18, 90.00
R / Rfree (%)	19.9 / 20.5

The structure of The Glic Pentameric Ligand-Gated Ion Channel Mutant Q193C also contains other interesting chemical elements:

Sodium

(Na)

6 atoms

The binding sites of Chlorine atom in the The Glic Pentameric Ligand-Gated Ion Channel Mutant Q193C (pdb code 6hy5). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 7 binding sites of Chlorine where determined in the The Glic Pentameric Ligand-Gated Ion Channel Mutant Q193C, PDB code: 6hy5:
Jump to Chlorine binding site number: 1; 2; 3; 4; 5; 6; 7;

Chlorine binding site 1 out of 7 in the The Glic Pentameric Ligand-Gated Ion Channel Mutant Q193C


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of The Glic Pentameric Ligand-Gated Ion Channel Mutant Q193C within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl404 b:44.4 occ:1.00 O E:HOH533 3.0 53.4 1.0 N A:PHE78 3.3 40.0 1.0 NH1 A:ARG85 3.5 57.4 1.0 N A:ARG85 3.6 54.4 1.0 CD2 A:PHE78 3.8 51.4 1.0 CB A:PHE78 3.9 43.1 1.0 CA A:ARG77 4.0 38.6 1.0 CG A:ARG85 4.1 55.2 1.0 CA A:ALA84 4.1 56.1 1.0 CG2 A:VAL81 4.1 45.0 1.0 CA A:PHE78 4.1 40.4 1.0 C A:ARG77 4.1 43.0 1.0 CB A:ARG85 4.2 50.6 1.0 O A:PHE78 4.3 40.8 1.0 CB A:ARG77 4.3 39.0 1.0 CG A:PHE78 4.3 46.9 1.0 CD A:ARG85 4.3 61.5 1.0 C A:ALA84 4.3 59.6 1.0 CA A:ARG85 4.5 53.4 1.0 CB A:ALA84 4.6 57.7 1.0 CG A:ARG77 4.6 46.5 1.0 CZ A:ARG85 4.6 69.0 1.0 C A:PHE78 4.7 40.7 1.0 CE2 A:PHE78 4.8 55.7 1.0 O A:HOH529 4.9 50.0 1.0 NE A:ARG85 4.9 66.3 1.0

Chlorine binding site 2 out of 7 in the The Glic Pentameric Ligand-Gated Ion Channel Mutant Q193C


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of The Glic Pentameric Ligand-Gated Ion Channel Mutant Q193C within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl405 b:88.4 occ:1.00 N A:LEU180 3.4 42.3 1.0 OD1 A:ASN139 3.4 84.2 1.0 CA A:ARG179 3.8 48.0 1.0 O A:ASP178 4.0 62.2 1.0 C A:ARG179 4.1 46.4 1.0 CB A:LEU180 4.1 41.0 1.0 CA A:LEU180 4.3 40.5 1.0 CD A:ARG179 4.4 76.0 1.0 CB A:ARG179 4.6 45.8 1.0 CG A:ASN139 4.6 91.4 1.0 CB A:SER134 4.7 55.6 1.0 N A:ARG179 4.7 50.8 1.0 C A:ASP178 4.7 59.7 1.0 CG A:ARG179 4.9 54.0 1.0

Chlorine binding site 3 out of 7 in the The Glic Pentameric Ligand-Gated Ion Channel Mutant Q193C


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of The Glic Pentameric Ligand-Gated Ion Channel Mutant Q193C within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl406 b:88.1 occ:1.00 N A:ALA175 3.5 47.5 1.0 CB A:ALA175 3.9 50.9 1.0 CA A:PHE174 4.3 45.4 1.0 CA A:ALA175 4.3 48.2 1.0 C A:PHE174 4.4 49.3 1.0 CB A:PHE174 4.5 45.0 1.0 O A:ALA175 4.8 53.4 1.0

Chlorine binding site 4 out of 7 in the The Glic Pentameric Ligand-Gated Ion Channel Mutant Q193C


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of The Glic Pentameric Ligand-Gated Ion Channel Mutant Q193C within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl404 b:58.1 occ:1.00 N B:PHE78 3.3 36.5 1.0 NH1 B:ARG85 3.6 48.2 1.0 N B:ARG85 3.6 57.3 1.0 CD2 B:PHE78 3.7 46.7 1.0 CB B:PHE78 3.9 38.7 1.0 CA B:ALA84 4.0 60.6 1.0 CG2 B:VAL81 4.1 39.8 1.0 CG B:ARG85 4.1 53.5 1.0 CA B:ARG77 4.1 35.0 1.0 CA B:PHE78 4.1 36.9 1.0 CB B:ARG85 4.2 48.9 1.0 C B:ARG77 4.2 38.6 1.0 CG B:PHE78 4.3 42.5 1.0 O B:PHE78 4.3 41.5 1.0 C B:ALA84 4.3 63.6 1.0 CD B:ARG85 4.4 57.5 1.0 CB B:ARG77 4.4 33.6 1.0 CA B:ARG85 4.5 55.2 1.0 CB B:ALA84 4.5 62.6 1.0 CZ B:ARG85 4.7 69.3 1.0 CG B:ARG77 4.7 45.5 1.0 C B:PHE78 4.7 41.2 1.0 CE2 B:PHE78 4.8 51.2 1.0 NE B:ARG85 4.9 61.3 1.0

Chlorine binding site 5 out of 7 in the The Glic Pentameric Ligand-Gated Ion Channel Mutant Q193C


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 5 of The Glic Pentameric Ligand-Gated Ion Channel Mutant Q193C within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl405 b:47.9 occ:1.00 N C:PHE78 3.4 43.4 1.0 N C:ARG85 3.7 62.6 1.0 NH1 C:ARG85 3.7 40.1 1.0 CA C:ALA84 3.9 64.0 1.0 CD2 C:PHE78 4.0 50.7 1.0 CB C:PHE78 4.0 44.4 1.0 CG2 C:VAL81 4.0 43.5 1.0 CA C:ARG77 4.1 43.3 1.0 O C:PHE78 4.2 47.6 1.0 C C:ARG77 4.2 48.0 1.0 CA C:PHE78 4.2 42.8 1.0 CB C:ARG77 4.2 40.2 1.0 CG C:ARG85 4.3 50.5 1.0 C C:ALA84 4.3 67.8 1.0 CB C:ALA84 4.4 66.0 1.0 CB C:ARG85 4.4 56.3 1.0 CG C:PHE78 4.5 47.2 1.0 CG C:ARG77 4.5 47.1 1.0 CD C:ARG85 4.6 50.2 1.0 CA C:ARG85 4.7 60.7 1.0 C C:PHE78 4.7 46.0 1.0 CZ C:ARG85 4.9 59.3 1.0

Chlorine binding site 6 out of 7 in the The Glic Pentameric Ligand-Gated Ion Channel Mutant Q193C


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 6 of The Glic Pentameric Ligand-Gated Ion Channel Mutant Q193C within 5.0Å range: probe atom residue distance (Å) B Occ D:Cl404 b:44.9 occ:1.00 N D:PHE78 3.3 40.1 1.0 N D:ARG85 3.5 52.6 1.0 NH1 D:ARG85 3.6 52.2 1.0 CD2 D:PHE78 3.7 49.2 1.0 CB D:PHE78 3.9 41.5 1.0 CA D:ALA84 4.0 55.4 1.0 CG D:ARG85 4.0 56.5 1.0 CA D:ARG77 4.0 38.7 1.0 CG2 D:VAL81 4.1 41.4 1.0 CA D:PHE78 4.1 39.6 1.0 C D:ARG77 4.1 44.3 1.0 CB D:ARG85 4.1 47.6 1.0 CG D:PHE78 4.3 45.0 1.0 C D:ALA84 4.3 57.7 1.0 O D:PHE78 4.3 42.8 1.0 CD D:ARG85 4.3 64.2 1.0 CB D:ARG77 4.4 37.4 1.0 CA D:ARG85 4.4 51.1 1.0 CB D:ALA84 4.5 57.1 1.0 CZ D:ARG85 4.7 73.5 1.0 CG D:ARG77 4.7 46.5 1.0 C D:PHE78 4.7 42.5 1.0 CE2 D:PHE78 4.8 53.1 1.0 NE D:ARG85 4.9 72.6 1.0

Chlorine binding site 7 out of 7 in the The Glic Pentameric Ligand-Gated Ion Channel Mutant Q193C


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 7 of The Glic Pentameric Ligand-Gated Ion Channel Mutant Q193C within 5.0Å range: probe atom residue distance (Å) B Occ E:Cl404 b:56.2 occ:1.00 N E:PHE78 3.2 43.7 1.0 NH1 E:ARG85 3.7 41.7 1.0 CD2 E:PHE78 3.8 50.6 1.0 N E:ARG85 3.8 55.9 1.0 CB E:PHE78 3.8 44.1 1.0 CA E:ARG77 4.0 45.4 1.0 CG2 E:VAL81 4.0 44.4 1.0 CA E:PHE78 4.0 42.5 1.0 C E:ARG77 4.1 48.6 1.0 O E:PHE78 4.1 42.9 1.0 CA E:ALA84 4.1 59.1 1.0 CB E:ARG77 4.2 46.7 1.0 CG E:PHE78 4.3 47.1 1.0 CG E:ARG85 4.3 53.5 1.0 CB E:ARG85 4.4 51.8 1.0 C E:ALA84 4.5 61.4 1.0 CD E:ARG85 4.5 46.7 1.0 CG E:ARG77 4.5 60.5 1.0 C E:PHE78 4.6 42.7 1.0 CB E:ALA84 4.6 61.2 1.0 CA E:ARG85 4.7 54.3 1.0 CZ E:ARG85 4.8 56.5 1.0 CE2 E:PHE78 4.9 53.9 1.0

H.Hu, K.Ataka, A.Menny, Z.Fourati, L.Sauguet, P.J.Corringer, P.Koehl, J.Heberle, M.Delarue. Electrostatics, Proton Sensor, and Networks Governing the Gating Transition in Glic, A Proton-Gated Pentameric Ion Channel. Proc. Natl. Acad. Sci. V. 115 12172 2018U.S.A..
ISSN: ESSN 1091-6490
PubMed: 30541892
DOI: 10.1073/PNAS.1813378116