Chlorine in PDB 6hz1: The Glic Pentameric Ligand-Gated Ion Channel Mutant E243C

The structure of The Glic Pentameric Ligand-Gated Ion Channel Mutant E243C, PDB code: 6hz1 was solved by H.D.Hu, M.Delarue, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.50
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	180.900, 132.950, 160.060, 90.00, 102.41, 90.00
R / Rfree (%)	20.5 / 21.6

The structure of The Glic Pentameric Ligand-Gated Ion Channel Mutant E243C also contains other interesting chemical elements:

Sodium

(Na)

6 atoms

The binding sites of Chlorine atom in the The Glic Pentameric Ligand-Gated Ion Channel Mutant E243C (pdb code 6hz1). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 7 binding sites of Chlorine where determined in the The Glic Pentameric Ligand-Gated Ion Channel Mutant E243C, PDB code: 6hz1:
Jump to Chlorine binding site number: 1; 2; 3; 4; 5; 6; 7;

Chlorine binding site 1 out of 7 in the The Glic Pentameric Ligand-Gated Ion Channel Mutant E243C


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of The Glic Pentameric Ligand-Gated Ion Channel Mutant E243C within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl404 b:47.6 occ:1.00 N A:PHE78 3.2 38.9 1.0 NH1 A:ARG85 3.7 65.5 1.0 N A:ARG85 3.8 64.7 1.0 CB A:PHE78 3.8 40.4 1.0 CD2 A:PHE78 3.9 47.0 1.0 CG2 A:VAL81 3.9 42.4 1.0 CA A:ARG77 4.0 37.9 1.0 CA A:PHE78 4.0 38.5 1.0 O A:PHE78 4.0 40.9 1.0 C A:ARG77 4.1 42.2 1.0 CA A:ALA84 4.1 65.5 1.0 CB A:ARG77 4.2 37.6 1.0 CG A:ARG85 4.3 64.9 1.0 CG A:PHE78 4.3 43.4 1.0 CB A:ARG85 4.4 61.3 1.0 C A:ALA84 4.5 69.3 1.0 CG A:ARG77 4.5 45.4 1.0 C A:PHE78 4.5 40.2 1.0 CB A:ALA84 4.5 66.7 1.0 CD A:ARG85 4.6 68.3 1.0 CA A:ARG85 4.7 63.5 1.0 CZ A:ARG85 4.8 80.2 1.0 CE2 A:PHE78 5.0 50.5 1.0

Chlorine binding site 2 out of 7 in the The Glic Pentameric Ligand-Gated Ion Channel Mutant E243C


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of The Glic Pentameric Ligand-Gated Ion Channel Mutant E243C within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl405 b:91.3 occ:1.00 OD1 A:ASN139 3.3 74.1 1.0 N A:LEU180 3.3 44.2 1.0 CA A:ARG179 3.7 46.6 1.0 C A:ARG179 4.0 48.9 1.0 CB A:LEU180 4.0 44.3 1.0 CG A:ASN139 4.2 80.4 1.0 O A:ASP178 4.3 54.4 1.0 CA A:LEU180 4.3 43.2 1.0 CD A:ARG179 4.3 67.6 1.0 CB A:SER134 4.4 50.3 1.0 CB A:ARG179 4.5 45.3 1.0 ND2 A:ASN139 4.5 75.3 1.0 N A:ARG179 4.7 47.8 1.0 CG A:ARG179 4.8 54.2 1.0 C A:ASP178 4.9 53.7 1.0 OG A:SER134 5.0 56.4 1.0

Chlorine binding site 3 out of 7 in the The Glic Pentameric Ligand-Gated Ion Channel Mutant E243C


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of The Glic Pentameric Ligand-Gated Ion Channel Mutant E243C within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl406 b:88.2 occ:1.00 N A:ALA175 3.4 42.0 1.0 CB A:ALA175 3.7 44.4 1.0 CA A:ALA175 4.1 42.2 1.0 CA A:PHE174 4.3 40.5 1.0 C A:PHE174 4.4 44.1 1.0 O A:ALA175 4.4 43.6 1.0 CB A:PHE174 4.5 41.4 1.0 C A:ALA175 4.8 44.6 1.0

Chlorine binding site 4 out of 7 in the The Glic Pentameric Ligand-Gated Ion Channel Mutant E243C


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of The Glic Pentameric Ligand-Gated Ion Channel Mutant E243C within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl405 b:64.6 occ:1.00 N B:PHE78 3.2 37.6 1.0 NH1 B:ARG85 3.7 52.1 1.0 N B:ARG85 3.8 70.6 1.0 CB B:PHE78 3.8 39.4 1.0 CD2 B:PHE78 3.9 46.6 1.0 CG2 B:VAL81 3.9 41.0 1.0 CA B:ARG77 4.0 37.5 1.0 CA B:PHE78 4.0 37.0 1.0 O B:PHE78 4.0 39.2 1.0 C B:ARG77 4.1 40.4 1.0 CA B:ALA84 4.1 72.3 1.0 CB B:ARG77 4.2 38.0 1.0 CG B:PHE78 4.3 42.7 1.0 CG B:ARG85 4.3 69.1 1.0 CB B:ARG85 4.5 66.7 1.0 C B:ALA84 4.5 76.1 1.0 CG B:ARG77 4.5 48.8 1.0 C B:PHE78 4.5 38.7 1.0 CB B:ALA84 4.5 74.1 1.0 CD B:ARG85 4.6 69.1 1.0 CA B:ARG85 4.8 69.1 1.0 CZ B:ARG85 4.8 73.8 1.0 CE2 B:PHE78 5.0 50.5 1.0

Chlorine binding site 5 out of 7 in the The Glic Pentameric Ligand-Gated Ion Channel Mutant E243C


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 5 of The Glic Pentameric Ligand-Gated Ion Channel Mutant E243C within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl404 b:55.7 occ:1.00 N C:PHE78 3.2 37.8 1.0 NH1 C:ARG85 3.7 70.6 1.0 N C:ARG85 3.8 68.2 1.0 CB C:PHE78 3.8 39.4 1.0 CD2 C:PHE78 3.9 45.9 1.0 CG2 C:VAL81 3.9 43.3 1.0 CA C:ARG77 4.0 38.2 1.0 O C:PHE78 4.0 39.1 1.0 CA C:PHE78 4.0 37.2 1.0 C C:ARG77 4.1 40.8 1.0 CA C:ALA84 4.1 69.0 1.0 CB C:ARG77 4.2 38.2 1.0 CG C:PHE78 4.3 42.3 1.0 CG C:ARG85 4.3 68.1 1.0 CB C:ARG85 4.5 65.2 1.0 CG C:ARG77 4.5 50.2 1.0 C C:ALA84 4.5 72.5 1.0 C C:PHE78 4.5 38.8 1.0 CB C:ALA84 4.6 70.6 1.0 CD C:ARG85 4.6 70.5 1.0 CA C:ARG85 4.8 67.3 1.0 CZ C:ARG85 4.9 84.5 1.0 CE2 C:PHE78 5.0 49.8 1.0

Chlorine binding site 6 out of 7 in the The Glic Pentameric Ligand-Gated Ion Channel Mutant E243C


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 6 of The Glic Pentameric Ligand-Gated Ion Channel Mutant E243C within 5.0Å range: probe atom residue distance (Å) B Occ D:Cl404 b:53.6 occ:1.00 N D:PHE78 3.2 38.1 1.0 NH1 D:ARG85 3.7 61.8 1.0 N D:ARG85 3.7 69.1 1.0 CB D:PHE78 3.8 40.3 1.0 CD2 D:PHE78 3.8 47.3 1.0 CG2 D:VAL81 3.9 41.2 1.0 CA D:ARG77 4.0 36.0 1.0 CA D:PHE78 4.0 38.0 1.0 O D:PHE78 4.0 40.4 1.0 C D:ARG77 4.1 40.7 1.0 CA D:ALA84 4.1 69.1 1.0 CB D:ARG77 4.2 35.4 1.0 CG D:ARG85 4.3 70.0 1.0 CG D:PHE78 4.3 43.4 1.0 CB D:ARG85 4.4 66.1 1.0 C D:ALA84 4.4 73.6 1.0 CG D:ARG77 4.5 44.6 1.0 CB D:ALA84 4.5 70.6 1.0 C D:PHE78 4.5 39.8 1.0 CD D:ARG85 4.6 74.0 1.0 CA D:ARG85 4.7 67.9 1.0 CZ D:ARG85 4.8 83.8 1.0 CE2 D:PHE78 4.9 50.9 1.0

Chlorine binding site 7 out of 7 in the The Glic Pentameric Ligand-Gated Ion Channel Mutant E243C


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 7 of The Glic Pentameric Ligand-Gated Ion Channel Mutant E243C within 5.0Å range: probe atom residue distance (Å) B Occ E:Cl405 b:69.0 occ:1.00 N E:PHE78 3.2 40.3 1.0 NH1 E:ARG85 3.7 62.2 1.0 N E:ARG85 3.8 69.3 1.0 CB E:PHE78 3.8 42.5 1.0 CD2 E:PHE78 3.9 48.8 1.0 CG2 E:VAL81 3.9 43.6 1.0 CA E:ARG77 4.0 40.1 1.0 CA E:PHE78 4.0 40.1 1.0 O E:PHE78 4.0 41.2 1.0 C E:ARG77 4.1 43.4 1.0 CA E:ALA84 4.1 70.3 1.0 CB E:ARG77 4.2 40.8 1.0 CG E:PHE78 4.3 45.5 1.0 CG E:ARG85 4.3 68.4 1.0 CB E:ARG85 4.4 66.2 1.0 C E:ALA84 4.5 73.3 1.0 CG E:ARG77 4.5 52.3 1.0 C E:PHE78 4.5 41.2 1.0 CB E:ALA84 4.6 71.8 1.0 CD E:ARG85 4.6 66.7 1.0 CA E:ARG85 4.7 68.3 1.0 CZ E:ARG85 4.9 76.2 1.0 CE2 E:PHE78 5.0 52.3 1.0

H.Hu, K.Ataka, A.Menny, Z.Fourati, L.Sauguet, P.J.Corringer, P.Koehl, J.Heberle, M.Delarue. Electrostatics, Proton Sensor, and Networks Governing the Gating Transition in Glic, A Proton-Gated Pentameric Ion Channel. Proc. Natl. Acad. Sci. V. 115 12172 2018U.S.A..
ISSN: ESSN 1091-6490
PubMed: 30541892
DOI: 10.1073/PNAS.1813378116