Chlorine in PDB 6j1a: Photoswitchable Fluorescent Protein Gamillus, Off-State

The structure of Photoswitchable Fluorescent Protein Gamillus, Off-State, PDB code: 6j1a was solved by R.Nakashima, K.Sakurai, H.Shinoda, T.Matsuda, T.Nagai, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	114.23 / 1.96
Space group	I 21 3
Cell size a, b, c (Å), α, β, γ (°)	161.540, 161.540, 161.540, 90.00, 90.00, 90.00
R / Rfree (%)	15.2 / 18

The binding sites of Chlorine atom in the Photoswitchable Fluorescent Protein Gamillus, Off-State (pdb code 6j1a). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 3 binding sites of Chlorine where determined in the Photoswitchable Fluorescent Protein Gamillus, Off-State, PDB code: 6j1a:
Jump to Chlorine binding site number: 1; 2; 3;

Chlorine binding site 1 out of 3 in the Photoswitchable Fluorescent Protein Gamillus, Off-State


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Photoswitchable Fluorescent Protein Gamillus, Off-State within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl301 b:41.8 occ:1.00 O A:HOH517 3.0 22.8 1.0 NE2 A:HIS74 3.4 20.7 1.0 SG A:CYS184 3.5 30.4 1.0 CD1 A:QYG70 3.5 35.3 1.0 NH2 A:ARG99 3.6 28.6 1.0 CD2 A:LEU164 3.7 31.2 1.0 CE1 A:HIS74 3.7 22.8 1.0 CB2 A:QYG70 3.8 33.5 1.0 CG2 A:QYG70 4.2 33.6 1.0 CB A:LEU164 4.3 23.5 1.0 O2 A:QYG70 4.3 23.1 1.0 CD A:GLN182 4.4 34.6 1.0 CB A:CYS184 4.4 25.4 1.0 NE2 A:GLN182 4.5 33.4 1.0 CE1 A:QYG70 4.5 42.6 1.0 CG A:LEU164 4.6 30.4 1.0 CG A:GLN182 4.7 28.4 1.0 CZ A:ARG99 4.7 29.9 1.0 CG1 A:VAL152 4.7 25.1 1.0 CD2 A:HIS74 4.7 23.1 1.0 OE1 A:GLN182 4.8 28.9 1.0 CA2 A:QYG70 4.9 27.0 1.0 C2 A:QYG70 5.0 25.9 1.0 NH1 A:ARG99 5.0 30.6 1.0

Chlorine binding site 2 out of 3 in the Photoswitchable Fluorescent Protein Gamillus, Off-State


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Photoswitchable Fluorescent Protein Gamillus, Off-State within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl302 b:31.2 occ:1.00 O A:HOH442 3.1 40.9 1.0 N A:THR84 3.1 24.7 1.0 N A:LYS198 3.2 25.4 1.0 OG1 A:THR84 3.5 34.1 1.0 CA A:PRO197 3.7 20.4 1.0 NZ A:LYS198 3.7 66.2 1.0 CG A:LYS198 3.8 44.5 1.0 CA A:ILE83 3.8 22.4 1.0 CB A:THR84 3.9 31.0 1.0 C A:ILE83 3.9 24.6 1.0 C A:PRO197 4.0 20.8 1.0 CG2 A:ILE83 4.1 22.4 1.0 CB A:LYS198 4.1 29.0 1.0 CA A:THR84 4.1 26.9 1.0 CB A:PRO197 4.2 22.7 1.0 CA A:LYS198 4.3 28.8 1.0 O A:ASP82 4.4 29.5 1.0 CB A:ILE83 4.5 22.8 1.0 O A:GLN196 4.8 25.1 1.0 CE A:LYS198 4.8 58.7 1.0 CD A:LYS198 4.8 60.6 1.0 N A:ILE83 4.9 25.1 1.0 N A:PRO197 5.0 24.0 1.0 O A:LYS198 5.0 23.2 1.0

Chlorine binding site 3 out of 3 in the Photoswitchable Fluorescent Protein Gamillus, Off-State


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Photoswitchable Fluorescent Protein Gamillus, Off-State within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl303 b:30.9 occ:1.00 O A:HOH453 3.0 22.0 1.0 O A:HOH523 3.1 36.1 1.0 N A:TRP62 3.2 19.3 1.0 CD1 A:TRP62 3.7 25.8 1.0 CB A:LYS142 3.8 35.3 1.0 CA A:SER61 3.9 25.1 1.0 CB A:TRP62 4.0 21.3 1.0 C A:SER61 4.0 22.2 1.0 CG A:LYS142 4.0 36.1 1.0 CA A:TRP62 4.1 21.5 1.0 OG A:SER61 4.1 24.6 1.0 CG A:TRP62 4.3 21.0 1.0 O A:HOH435 4.3 44.1 1.0 CB A:SER61 4.5 23.4 1.0 CD A:LYS142 4.5 48.9 1.0 CA A:LYS142 4.6 29.1 1.0 N A:VAL63 4.7 21.4 1.0 O A:LYS142 4.7 28.3 1.0 O A:HOH456 4.7 27.1 1.0 O A:MET60 4.7 23.9 1.0 NE1 A:TRP62 4.9 24.7 1.0 O A:HOH441 4.9 38.2 1.0 N A:SER61 5.0 27.2 1.0 C A:TRP62 5.0 21.3 1.0 C A:LYS142 5.0 28.8 1.0

H.Shinoda, K.Lu, R.Nakashima, T.Wazawa, K.Noguchi, T.Matsuda, T.Nagai. Acid-Tolerant Reversibly Switchable Green Fluorescent Protein For Super-Resolution Imaging Under Acidic Conditions. Cell Chem Biol V. 26 1469 2019.
ISSN: ESSN 2451-9456
PubMed: 31422907
DOI: 10.1016/J.CHEMBIOL.2019.07.012