Chlorine in PDB 6j1c: Photoswitchable Fluorescent Protein Gamillus, N150C/T204V Double Mutant, Off-State

The structure of Photoswitchable Fluorescent Protein Gamillus, N150C/T204V Double Mutant, Off-State, PDB code: 6j1c was solved by R.Nakashima, H.Shinoda, T.Matsuda, T.Nagai, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.77 / 2.09
Space group	I 21 3
Cell size a, b, c (Å), α, β, γ (°)	162.021, 162.021, 162.021, 90.00, 90.00, 90.00
R / Rfree (%)	16.1 / 17.7

The binding sites of Chlorine atom in the Photoswitchable Fluorescent Protein Gamillus, N150C/T204V Double Mutant, Off-State (pdb code 6j1c). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 3 binding sites of Chlorine where determined in the Photoswitchable Fluorescent Protein Gamillus, N150C/T204V Double Mutant, Off-State, PDB code: 6j1c:
Jump to Chlorine binding site number: 1; 2; 3;

Chlorine binding site 1 out of 3 in the Photoswitchable Fluorescent Protein Gamillus, N150C/T204V Double Mutant, Off-State


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Photoswitchable Fluorescent Protein Gamillus, N150C/T204V Double Mutant, Off-State within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl301 b:35.0 occ:1.00 O A:HOH443 3.0 26.4 1.0 O A:HOH545 3.0 37.9 1.0 N A:TRP62 3.2 26.0 1.0 CD1 A:TRP62 3.7 30.4 1.0 CA A:SER61 3.9 26.4 1.0 CB A:TRP62 3.9 28.5 1.0 CB A:LYS142 4.0 38.9 1.0 C A:SER61 4.0 27.6 1.0 CA A:TRP62 4.1 27.6 1.0 O A:HOH532 4.2 46.7 1.0 OG A:SER61 4.2 27.8 1.0 CG A:LYS142 4.2 40.1 1.0 CG A:TRP62 4.2 28.7 1.0 O A:HOH445 4.4 46.7 1.0 CB A:SER61 4.5 29.0 1.0 O A:HOH516 4.5 35.6 1.0 CD A:LYS142 4.6 48.0 1.0 CA A:LYS142 4.6 34.6 1.0 N A:VAL63 4.7 27.8 1.0 O A:MET60 4.7 29.1 1.0 O A:LYS142 4.8 32.8 1.0 NE1 A:TRP62 4.9 29.6 1.0 N A:SER61 4.9 30.1 1.0 C A:TRP62 5.0 24.8 1.0

Chlorine binding site 2 out of 3 in the Photoswitchable Fluorescent Protein Gamillus, N150C/T204V Double Mutant, Off-State


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Photoswitchable Fluorescent Protein Gamillus, N150C/T204V Double Mutant, Off-State within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl302 b:38.0 occ:1.00 O A:HOH439 3.2 47.5 1.0 N A:LYS198 3.2 29.2 1.0 N A:THR84 3.3 29.4 1.0 OG1 A:THR84 3.6 44.3 1.0 CA A:PRO197 3.7 27.9 1.0 CG A:LYS198 3.8 53.3 1.0 CA A:ILE83 3.8 29.1 1.0 C A:PRO197 3.9 32.3 1.0 CB A:LYS198 4.0 36.5 1.0 C A:ILE83 4.0 32.3 1.0 CB A:THR84 4.0 38.0 1.0 CG2 A:ILE83 4.1 30.6 1.0 CB A:PRO197 4.1 28.0 1.0 CA A:LYS198 4.2 35.1 1.0 CA A:THR84 4.2 32.6 1.0 O A:ASP82 4.3 36.1 1.0 CD A:LYS198 4.5 70.5 1.0 CB A:ILE83 4.5 27.7 1.0 N A:ILE83 4.9 35.3 1.0 O A:GLN196 4.9 29.5 1.0 O A:LYS198 4.9 28.6 1.0 N A:PRO197 5.0 27.2 1.0 C A:ASP82 5.0 40.5 1.0

Chlorine binding site 3 out of 3 in the Photoswitchable Fluorescent Protein Gamillus, N150C/T204V Double Mutant, Off-State


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Photoswitchable Fluorescent Protein Gamillus, N150C/T204V Double Mutant, Off-State within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl303 b:39.8 occ:1.00 O A:HOH523 3.0 25.0 1.0 NE2 A:HIS74 3.3 26.3 1.0 CD1 A:QYG70 3.5 35.6 1.0 SG A:CYS184 3.6 34.9 1.0 NH2 A:ARG99 3.7 33.2 1.0 CE1 A:HIS74 3.7 31.5 1.0 CD2 A:LEU164 3.8 41.4 1.0 CB2 A:QYG70 3.9 33.3 1.0 CG2 A:QYG70 4.2 33.1 1.0 O2 A:QYG70 4.3 28.4 1.0 CB A:LEU164 4.3 29.8 1.0 CD A:GLN182 4.4 32.1 1.0 CB A:CYS184 4.4 34.0 1.0 NE2 A:GLN182 4.5 29.4 1.0 CD2 A:HIS74 4.5 26.9 1.0 CG A:GLN182 4.6 29.8 1.0 CG1 A:VAL152 4.6 29.4 1.0 CE1 A:QYG70 4.6 38.7 1.0 CG A:LEU164 4.7 34.4 1.0 CG2 A:VAL152 4.8 26.9 1.0 OE1 A:GLN182 4.8 28.7 1.0 CZ A:ARG99 4.8 32.7 1.0 OG A:SER166 5.0 29.3 1.0

H.Shinoda, K.Lu, R.Nakashima, T.Wazawa, K.Noguchi, T.Matsuda, T.Nagai. Acid-Tolerant Reversibly Switchable Green Fluorescent Protein For Super-Resolution Imaging Under Acidic Conditions. Cell Chem Biol V. 26 1469 2019.
ISSN: ESSN 2451-9456
PubMed: 31422907
DOI: 10.1016/J.CHEMBIOL.2019.07.012