Chlorine in PDB 6jgh: Crystal Structure of the F99S/M153T/V163A/T203I Variant of Gfp at 0.94 A

The structure of Crystal Structure of the F99S/M153T/V163A/T203I Variant of Gfp at 0.94 A, PDB code: 6jgh was solved by H.Eki, Y.Tai, K.Takaba, Y.Hanazono, K.Miki, K.Takeda, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.93 / 0.94
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	50.641, 62.506, 68.179, 90.00, 90.00, 90.00
R / Rfree (%)	10.7 / 12.9

The binding sites of Chlorine atom in the Crystal Structure of the F99S/M153T/V163A/T203I Variant of Gfp at 0.94 A (pdb code 6jgh). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of the F99S/M153T/V163A/T203I Variant of Gfp at 0.94 A, PDB code: 6jgh:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Crystal Structure of the F99S/M153T/V163A/T203I Variant of Gfp at 0.94 A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of the F99S/M153T/V163A/T203I Variant of Gfp at 0.94 A within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl301 b:18.1 occ:0.30 CL A:CL301 0.0 18.1 0.3 CL A:CL301 0.6 6.9 0.4 O A:HOH634 2.5 9.2 0.3 H A:TRP57 2.6 5.4 1.0 O A:HOH615 2.7 11.3 1.0 O A:HOH654 2.7 11.3 0.8 HD1 A:TRP57 2.7 5.8 1.0 HD1 A:TYR143 2.8 7.7 1.0 HB2 A:TRP57 2.8 5.5 1.0 HD3 A:PRO58 2.9 5.5 1.0 O A:HOH654 3.1 11.9 0.2 N A:TRP57 3.3 4.5 1.0 O A:HOH546 3.4 11.2 1.0 HA A:PRO56 3.5 5.7 1.0 CD1 A:TRP57 3.5 4.8 1.0 CD1 A:TYR143 3.6 6.5 1.0 O A:HOH634 3.6 11.1 0.5 HD2 A:PRO58 3.6 5.5 1.0 CB A:TRP57 3.6 4.5 1.0 CD A:PRO58 3.7 4.6 1.0 HB3 A:PRO56 3.8 6.2 1.0 HE1 A:TYR143 3.9 7.4 1.0 CG A:TRP57 4.0 4.2 1.0 O A:HOH762 4.0 25.3 0.7 CA A:TRP57 4.1 4.1 1.0 HB2 A:TYR143 4.1 7.9 1.0 CA A:PRO56 4.1 4.8 1.0 CE1 A:TYR143 4.1 6.2 1.0 HZ1 A:LYS209 4.2 11.7 1.0 C A:PRO56 4.2 4.2 1.0 O A:HOH597 4.4 12.9 0.6 HB3 A:TRP57 4.4 5.5 1.0 CB A:PRO56 4.4 5.2 1.0 O A:HOH854 4.5 23.6 0.8 CG A:TYR143 4.5 5.6 1.0 HA A:TYR143 4.6 7.0 1.0 CG A:PRO58 4.7 5.1 1.0 HG2 A:PRO58 4.7 6.1 1.0 N A:PRO58 4.7 4.2 1.0 O A:HOH550 4.7 17.9 0.1 CB A:TYR143 4.7 6.6 1.0 H1 A:HOH600 4.8 10.7 1.0 O A:HOH600 4.8 7.2 1.0 NE1 A:TRP57 4.8 5.1 1.0 HG3 A:PRO58 4.8 6.1 1.0 O A:HOH781 4.8 33.3 0.7 HA A:TRP57 4.8 5.0 1.0 HB2 A:PRO56 4.9 6.2 1.0 C A:TRP57 4.9 4.1 1.0 NZ A:LYS209 5.0 7.8 1.0 HD1 A:HIS139 5.0 10.0 1.0 HZ2 A:LYS209 5.0 11.7 1.0

Chlorine binding site 2 out of 2 in the Crystal Structure of the F99S/M153T/V163A/T203I Variant of Gfp at 0.94 A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of the F99S/M153T/V163A/T203I Variant of Gfp at 0.94 A within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl301 b:6.9 occ:0.41 CL A:CL301 0.0 6.9 0.4 CL A:CL301 0.6 18.1 0.3 O A:HOH634 2.0 9.2 0.3 H A:TRP57 2.4 5.4 1.0 O A:HOH654 2.8 11.3 0.8 HD1 A:TRP57 2.9 5.8 1.0 O A:HOH546 2.9 11.2 1.0 O A:HOH615 3.0 11.3 1.0 HA A:PRO56 3.0 5.7 1.0 HD3 A:PRO58 3.1 5.5 1.0 HB2 A:TRP57 3.1 5.5 1.0 O A:HOH634 3.1 11.1 0.5 N A:TRP57 3.1 4.5 1.0 O A:HOH654 3.2 11.9 0.2 HD1 A:TYR143 3.3 7.7 1.0 HB3 A:PRO56 3.5 6.2 1.0 HD2 A:PRO58 3.6 5.5 1.0 CD1 A:TRP57 3.6 4.8 1.0 CA A:PRO56 3.7 4.8 1.0 CD A:PRO58 3.8 4.6 1.0 CB A:TRP57 3.8 4.5 1.0 C A:PRO56 3.9 4.2 1.0 CA A:TRP57 4.0 4.1 1.0 CB A:PRO56 4.1 5.2 1.0 CG A:TRP57 4.1 4.2 1.0 CD1 A:TYR143 4.1 6.5 1.0 O A:HOH762 4.1 25.3 0.7 O A:HOH597 4.3 12.9 0.6 HE1 A:TYR143 4.4 7.4 1.0 HD1 A:HIS139 4.4 10.0 1.0 O A:HOH550 4.4 17.9 0.1 O A:HOH600 4.4 7.2 1.0 O A:HOH781 4.5 33.3 0.7 HZ1 A:LYS209 4.5 11.7 1.0 H1 A:HOH600 4.5 10.7 1.0 HB2 A:TYR143 4.5 7.9 1.0 HB2 A:PRO56 4.6 6.2 1.0 HB3 A:TRP57 4.6 5.5 1.0 CE1 A:TYR143 4.7 6.2 1.0 HA A:TRP57 4.8 5.0 1.0 N A:PRO58 4.8 4.2 1.0 NE1 A:TRP57 4.8 5.1 1.0 O A:HOH854 4.8 23.6 0.8 HG2 A:PRO58 4.9 6.1 1.0 CG A:PRO58 4.9 5.1 1.0 HA A:TYR143 5.0 7.0 1.0 C A:TRP57 5.0 4.1 1.0 O A:VAL55 5.0 5.4 1.0

K.Takaba, Y.Tai, H.Eki, H.A.Dao, Y.Hanazono, K.Hasegawa, K.Miki, K.Takeda. Subatomic Resolution X-Ray Structures of Green Fluorescent Protein. Iucrj V. 6 387 2019.
ISSN: ESSN 2052-2525
PubMed: 31098020
DOI: 10.1107/S205225251900246X