Chlorine in PDB 6nju: Mouse Endonuclease G Mutant H97A Bound to A-Dna

The structure of Mouse Endonuclease G Mutant H97A Bound to A-Dna, PDB code: 6nju was solved by C.M.Vander Zanden, E.N.Ho, R.S.Czarny, A.B.Robertson, P.S.Ho, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	53.99 / 2.35
Space group	P 65 2 2
Cell size a, b, c (Å), α, β, γ (°)	107.983, 107.983, 357.419, 90.00, 90.00, 120.00
R / Rfree (%)	22.2 / 29.2

The structure of Mouse Endonuclease G Mutant H97A Bound to A-Dna also contains other interesting chemical elements:

Magnesium

(Mg)

4 atoms

The binding sites of Chlorine atom in the Mouse Endonuclease G Mutant H97A Bound to A-Dna (pdb code 6nju). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the Mouse Endonuclease G Mutant H97A Bound to A-Dna, PDB code: 6nju:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in the Mouse Endonuclease G Mutant H97A Bound to A-Dna


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Mouse Endonuclease G Mutant H97A Bound to A-Dna within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl302 b:54.8 occ:1.00 NE1 A:TRP106 3.2 53.2 1.0 ND1 A:HIS104 3.2 39.3 1.0 CD A:ARG59 3.5 41.4 0.5 CE1 A:HIS104 3.7 36.2 1.0 CG A:ARG59 4.0 43.2 0.5 CA A:SER36 4.0 35.1 1.0 CD1 A:TRP106 4.0 49.2 1.0 CG A:ARG59 4.1 43.2 0.6 CB A:SER36 4.1 37.4 1.0 CE2 A:TRP106 4.1 53.5 1.0 NE A:ARG59 4.2 35.8 0.5 CB A:ARG59 4.3 46.1 0.5 O A:GLU35 4.3 40.0 1.0 CB A:ARG59 4.3 46.1 0.6 CG A:HIS104 4.4 43.9 1.0 O A:SER36 4.5 32.5 1.0 CZ2 A:TRP106 4.5 45.0 1.0 CD A:ARG59 4.6 47.3 0.6 NE A:ARG59 4.7 42.8 0.6 C A:SER36 4.7 37.1 1.0 NH1 A:ARG59 4.8 32.2 0.5 CZ A:ARG59 4.8 34.0 0.5 O A:GLN54 4.8 33.2 1.0 CB A:HIS104 4.9 33.1 1.0 CB A:ALA110 4.9 38.1 1.0 N A:SER36 5.0 41.9 1.0

Chlorine binding site 2 out of 4 in the Mouse Endonuclease G Mutant H97A Bound to A-Dna


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Mouse Endonuclease G Mutant H97A Bound to A-Dna within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl302 b:57.3 occ:1.00 NE1 C:TRP106 3.1 45.0 1.0 ND1 C:HIS104 3.2 28.9 1.0 CD C:ARG59 3.6 36.8 1.0 CA C:SER36 3.8 33.5 1.0 CD1 C:TRP106 3.8 37.7 1.0 CE1 C:HIS104 3.8 29.9 1.0 O C:GLU35 4.0 40.0 1.0 CB C:SER36 4.0 35.5 1.0 CE2 C:TRP106 4.0 41.0 1.0 CG C:HIS104 4.4 35.4 1.0 CG C:ARG59 4.4 43.6 1.0 CB C:ARG59 4.5 39.8 1.0 CZ2 C:TRP106 4.5 46.5 1.0 O C:SER36 4.5 32.8 1.0 NE C:ARG59 4.5 50.6 1.0 CB C:ALA110 4.5 43.7 1.0 C C:SER36 4.6 34.6 1.0 NH1 C:ARG59 4.7 48.1 1.0 C C:GLU35 4.7 39.2 1.0 N C:SER36 4.8 31.1 1.0 CB C:HIS104 4.8 35.2 1.0 CZ C:ARG59 5.0 54.0 1.0 CG C:TRP106 5.0 37.6 1.0

Chlorine binding site 3 out of 4 in the Mouse Endonuclease G Mutant H97A Bound to A-Dna


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Mouse Endonuclease G Mutant H97A Bound to A-Dna within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl303 b:53.6 occ:1.00 N C:HIS78 3.4 23.0 1.0 CB C:HIS78 3.6 30.2 1.0 ND1 C:HIS81 4.1 27.9 1.0 CA C:HIS78 4.1 24.6 1.0 O D:HOH403 4.3 31.4 1.0 CG1 C:VAL77 4.3 24.2 1.0 CA C:VAL77 4.3 26.6 1.0 C C:VAL77 4.3 25.6 1.0 NH2 C:ARG32 4.6 44.4 1.0 CD1 D:LEU233 4.7 29.1 1.0 CG C:GLN30 4.7 71.6 1.0 CB C:VAL77 4.9 28.6 1.0 CG C:HIS78 4.9 29.8 1.0 CB C:PRO43 4.9 26.7 1.0 CD C:GLN30 5.0 84.0 1.0 CE1 C:HIS81 5.0 27.1 1.0 CB C:HIS81 5.0 29.7 1.0

Chlorine binding site 4 out of 4 in the Mouse Endonuclease G Mutant H97A Bound to A-Dna


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Mouse Endonuclease G Mutant H97A Bound to A-Dna within 5.0Å range: probe atom residue distance (Å) B Occ D:Cl302 b:51.6 occ:1.00 N D:GLY175 3.0 27.3 1.0 CA D:ILE174 3.7 21.0 1.0 O D:HOH414 3.8 26.6 1.0 C D:ILE174 3.8 23.3 1.0 CA D:GLY175 3.9 25.6 1.0 O D:HOH420 4.1 26.4 1.0 CB D:ILE174 4.1 19.4 1.0 C D:GLY175 4.6 29.2 1.0 O D:VAL173 4.6 20.4 1.0 CG2 D:ILE174 4.7 22.9 1.0 O D:GLY175 4.7 24.4 1.0 N D:ILE174 4.9 26.8 1.0

C.M.Vander Zanden, E.N.Ho, R.S.Czarny, A.B.Robertson, P.S.Ho. Structural Adaptation of Vertebrate Endonuclease G For 5-Hydroxymethylcytosine Recognition and Function To Be Published.