Chlorine in PDB 6os0: Structure of Synthetic Nanobody-Stabilized Angiotensin II Type 1 Receptor Bound to Angiotensin II

The structure of Structure of Synthetic Nanobody-Stabilized Angiotensin II Type 1 Receptor Bound to Angiotensin II, PDB code: 6os0 was solved by L.M.Wingler, D.P.Staus, M.A.Skiba, C.Mcmahon, A.L.W.Kleinhenz, R.J.Lefkowitz, A.C.Kruse, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	37.20 / 2.90
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	135.530, 37.830, 111.920, 90.00, 94.41, 90.00
R / Rfree (%)	25.9 / 31.8

The binding sites of Chlorine atom in the Structure of Synthetic Nanobody-Stabilized Angiotensin II Type 1 Receptor Bound to Angiotensin II (pdb code 6os0). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Structure of Synthetic Nanobody-Stabilized Angiotensin II Type 1 Receptor Bound to Angiotensin II, PDB code: 6os0:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Structure of Synthetic Nanobody-Stabilized Angiotensin II Type 1 Receptor Bound to Angiotensin II


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Structure of Synthetic Nanobody-Stabilized Angiotensin II Type 1 Receptor Bound to Angiotensin II within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl2002 b:73.3 occ:1.00 CA A:HIS132 3.4 75.1 1.0 CG A:LYS135 3.5 67.4 1.0 CB A:HIS132 3.5 85.5 1.0 N A:LYS135 3.6 67.9 1.0 C A:HIS132 3.7 59.5 1.0 CB A:LYS135 3.8 82.2 1.0 CD A:LYS135 3.9 78.0 1.0 N A:MET134 4.0 67.2 1.0 N A:PRO133 4.1 56.9 1.0 CB A:MET134 4.2 62.7 1.0 ND1 A:HIS132 4.3 82.9 1.0 O A:HIS132 4.3 51.1 1.0 CA A:LYS135 4.4 72.8 1.0 CD A:PRO133 4.4 61.8 1.0 CG A:HIS132 4.4 77.7 1.0 CE A:LYS135 4.4 75.4 1.0 CA A:MET134 4.4 54.0 1.0 C A:MET134 4.5 60.5 1.0 O A:VAL131 4.5 70.7 1.0 N A:HIS132 4.7 64.4 1.0 CG A:MET134 4.7 70.7 1.0 C A:PRO133 4.9 61.0 1.0

Chlorine binding site 2 out of 2 in the Structure of Synthetic Nanobody-Stabilized Angiotensin II Type 1 Receptor Bound to Angiotensin II


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Structure of Synthetic Nanobody-Stabilized Angiotensin II Type 1 Receptor Bound to Angiotensin II within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl2003 b:78.5 occ:1.00 N D:TYR109 3.3 64.9 1.0 CB A:LYS1308 3.4 91.6 1.0 N A:LYS1308 3.4 77.2 1.0 N A:PHE1309 3.6 92.0 1.0 OD1 D:ASP108 3.6 73.7 1.0 CA A:GLY1306 3.6 66.1 1.0 N D:ASP110 3.7 74.7 1.0 CA A:LYS1308 3.8 77.3 1.0 C A:GLY1306 3.8 66.0 1.0 CA D:ASP108 4.0 47.1 1.0 N A:LYS1307 4.0 56.8 1.0 CD2 D:TYR109 4.0 56.5 1.0 CA D:TYR109 4.1 60.2 1.0 C D:ASP108 4.1 63.8 1.0 C A:LYS1308 4.1 94.3 1.0 CB D:ASP110 4.2 84.9 1.0 CB D:TYR109 4.2 49.6 1.0 C D:TYR109 4.3 70.3 1.0 CG D:ASP108 4.3 72.6 1.0 O A:GLY1306 4.4 67.2 1.0 CB A:PHE1309 4.4 75.6 1.0 C A:LYS1307 4.5 86.2 1.0 CG D:TYR109 4.6 57.9 1.0 CA A:PHE1309 4.6 74.3 1.0 SD A:MET57 4.6 98.8 1.0 CA D:ASP110 4.6 71.2 1.0 CB D:ASP108 4.8 61.8 1.0 O D:PHE107 4.8 53.0 1.0 CA A:LYS1307 4.9 81.6 1.0 N A:GLY1306 5.0 65.7 1.0 N D:ASP108 5.0 53.3 1.0 CE2 D:TYR109 5.0 52.6 1.0

L.M.Wingler, M.A.Skiba, C.Mcmahon, D.P.Staus, A.L.W.Kleinhenz, C.-M.Suomivuori, N.R.Latorraca, R.O.Dror, R.J.Lefkowitz, A.C.Kruse. Angiotensin and Biased Analogs Induce Structurally Distinct Active Conformations Within A Gpcr Science 2020.
ISSN: ESSN 1095-9203
DOI: 10.1126/SCIENCE.AAY9813