Chlorine in PDB 6p1m: Binary Complex of Human Dna Polymerase Mu with 1-Nt Gapped Substrate Containing Template 8OG

All present enzymatic activity of Binary Complex of Human Dna Polymerase Mu with 1-Nt Gapped Substrate Containing Template 8OG:
2.7.7.7;

The structure of Binary Complex of Human Dna Polymerase Mu with 1-Nt Gapped Substrate Containing Template 8OG, PDB code: 6p1m was solved by A.M.Kaminski, L.C.Pedersen, K.Bebenek, K.K.Chiruvella, D.A.Ramsden, T.A.Kunkel, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	26.13 / 1.65
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	59.929, 68.263, 110.413, 90.00, 90.00, 90.00
R / Rfree (%)	16.6 / 19

The structure of Binary Complex of Human Dna Polymerase Mu with 1-Nt Gapped Substrate Containing Template 8OG also contains other interesting chemical elements:

Potassium	(K)	1 atom
Bromine	(Br)	2 atoms

The binding sites of Chlorine atom in the Binary Complex of Human Dna Polymerase Mu with 1-Nt Gapped Substrate Containing Template 8OG (pdb code 6p1m). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Binary Complex of Human Dna Polymerase Mu with 1-Nt Gapped Substrate Containing Template 8OG, PDB code: 6p1m:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Binary Complex of Human Dna Polymerase Mu with 1-Nt Gapped Substrate Containing Template 8OG


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Binary Complex of Human Dna Polymerase Mu with 1-Nt Gapped Substrate Containing Template 8OG within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl503 b:19.6 occ:1.00 O D:HOH111 3.2 43.9 1.0 N A:GLY174 3.4 18.9 1.0 NH1 A:ARG175 3.4 30.7 1.0 N A:ARG175 3.5 16.2 1.0 CB A:SER172 3.5 28.4 1.0 CA A:SER172 3.5 22.1 1.0 C A:SER172 3.6 19.2 1.0 CA A:GLY174 3.7 18.7 1.0 CG A:ARG175 3.9 18.6 1.0 N A:GLU173 3.9 19.9 1.0 O6 D:DG1 4.0 21.8 1.0 O A:SER172 4.0 18.6 1.0 C A:GLY174 4.1 17.0 1.0 CD A:ARG175 4.3 24.3 1.0 OG A:SER172 4.3 34.2 1.0 CB A:ARG175 4.3 16.9 1.0 C6 D:DG1 4.4 19.5 1.0 C A:GLU173 4.4 18.8 1.0 CA A:ARG175 4.5 17.0 1.0 CZ A:ARG175 4.6 31.6 1.0 C5 D:DG1 4.7 19.5 1.0 CA A:GLU173 4.8 20.2 1.0 N7 D:DG1 4.8 19.8 1.0 NE A:ARG175 4.9 29.0 1.0 N4 T:DC4 5.0 21.3 1.0 N A:SER172 5.0 22.8 1.0

Chlorine binding site 2 out of 2 in the Binary Complex of Human Dna Polymerase Mu with 1-Nt Gapped Substrate Containing Template 8OG


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Binary Complex of Human Dna Polymerase Mu with 1-Nt Gapped Substrate Containing Template 8OG within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl505 b:23.9 occ:0.57 O A:HOH659 2.3 37.4 1.0 O T:HOH116 2.6 29.3 1.0 NH1 A:ARG446 3.3 22.1 1.0 CD A:ARG446 3.5 15.8 1.0 C5' T:DC4 3.8 17.6 1.0 CG A:ARG446 3.8 15.7 1.0 O T:HOH118 3.8 19.1 1.0 C4' T:DC4 3.9 17.6 1.0 CG A:LYS450 4.0 24.5 1.0 CE A:LYS450 4.0 30.0 1.0 O D:HOH107 4.0 30.1 1.0 O4' T:DC4 4.1 19.0 1.0 CZ A:ARG446 4.2 19.7 1.0 NE A:ARG446 4.3 17.2 1.0 CD A:LYS450 4.4 28.2 1.0 O T:HOH137 4.6 37.1 0.9 O A:HOH808 4.6 28.6 1.0 CB A:LYS450 5.0 19.6 1.0

A.M.Kaminski, K.K.Chiruvella, D.A.Ramsden, T.A.Kunkel, K.Bebenek, L.C.Pedersen. Unexpected Behavior of Dna Polymerase Mu Opposite Template 8-Oxo-7,8-Dihydro-2'-Guanosine. Nucleic Acids Res. V. 47 9410 2019.
ISSN: ESSN 1362-4962
PubMed: 31435651
DOI: 10.1093/NAR/GKZ680