Chlorine in PDB 6p4b: HYHEL10 Fab Variant HYHEL10-4X (Heavy Chain Mutations L4F, Y33H, S56N, and Y58F) Bound to Hen Egg Lysozyme Variant HEL2X-Flex (Mutations R21Q, R73E, C76S, and C94S)

Enzymatic activity of HYHEL10 Fab Variant HYHEL10-4X (Heavy Chain Mutations L4F, Y33H, S56N, and Y58F) Bound to Hen Egg Lysozyme Variant HEL2X-Flex (Mutations R21Q, R73E, C76S, and C94S)

All present enzymatic activity of HYHEL10 Fab Variant HYHEL10-4X (Heavy Chain Mutations L4F, Y33H, S56N, and Y58F) Bound to Hen Egg Lysozyme Variant HEL2X-Flex (Mutations R21Q, R73E, C76S, and C94S):
3.2.1.17;

Protein crystallography data

The structure of HYHEL10 Fab Variant HYHEL10-4X (Heavy Chain Mutations L4F, Y33H, S56N, and Y58F) Bound to Hen Egg Lysozyme Variant HEL2X-Flex (Mutations R21Q, R73E, C76S, and C94S), PDB code: 6p4b was solved by D.B.Langley, D.Christ, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 34.91 / 1.90
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 66.397, 65.722, 137.128, 90.00, 98.48, 90.00
R / Rfree (%) 20.3 / 24.4

Chlorine Binding Sites:

The binding sites of Chlorine atom in the HYHEL10 Fab Variant HYHEL10-4X (Heavy Chain Mutations L4F, Y33H, S56N, and Y58F) Bound to Hen Egg Lysozyme Variant HEL2X-Flex (Mutations R21Q, R73E, C76S, and C94S) (pdb code 6p4b). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 9 binding sites of Chlorine where determined in the HYHEL10 Fab Variant HYHEL10-4X (Heavy Chain Mutations L4F, Y33H, S56N, and Y58F) Bound to Hen Egg Lysozyme Variant HEL2X-Flex (Mutations R21Q, R73E, C76S, and C94S), PDB code: 6p4b:
Jump to Chlorine binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9;

Chlorine binding site 1 out of 9 in 6p4b

Go back to Chlorine Binding Sites List in 6p4b
Chlorine binding site 1 out of 9 in the HYHEL10 Fab Variant HYHEL10-4X (Heavy Chain Mutations L4F, Y33H, S56N, and Y58F) Bound to Hen Egg Lysozyme Variant HEL2X-Flex (Mutations R21Q, R73E, C76S, and C94S)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of HYHEL10 Fab Variant HYHEL10-4X (Heavy Chain Mutations L4F, Y33H, S56N, and Y58F) Bound to Hen Egg Lysozyme Variant HEL2X-Flex (Mutations R21Q, R73E, C76S, and C94S) within 5.0Å range:
probe atom residue distance (Å) B Occ
L:Cl301

b:56.2
occ:1.00
 NH2 L:ARG61 3.4 40.5 1.0
CB L:PRO59 3.4 41.5 1.0
N L:ARG61 3.4 36.6 1.0
CG L:ARG61 3.6 36.9 1.0
N L:SER60 3.6 45.6 1.0
CB L:ARG61 3.8 35.3 1.0
C L:PRO59 3.9 47.6 1.0
CA L:PRO59 4.0 43.4 1.0
CD L:ARG61 4.1 39.1 1.0
CA L:ARG61 4.2 36.8 1.0
CA L:SER60 4.3 42.8 1.0
C L:SER60 4.3 38.3 1.0
CB L:SER60 4.4 47.8 1.0
CZ L:ARG61 4.5 37.6 1.0
CG L:PRO59 4.6 43.7 1.0
O L:PRO59 4.7 41.6 1.0
NE L:ARG61 4.7 39.1 1.0
OE2 L:GLU79 5.0 48.8 1.0
O L:HOH406 5.0 45.7 1.0
O L:HOH454 5.0 42.9 1.0

Chlorine binding site 2 out of 9 in 6p4b

Go back to Chlorine Binding Sites List in 6p4b
Chlorine binding site 2 out of 9 in the HYHEL10 Fab Variant HYHEL10-4X (Heavy Chain Mutations L4F, Y33H, S56N, and Y58F) Bound to Hen Egg Lysozyme Variant HEL2X-Flex (Mutations R21Q, R73E, C76S, and C94S)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of HYHEL10 Fab Variant HYHEL10-4X (Heavy Chain Mutations L4F, Y33H, S56N, and Y58F) Bound to Hen Egg Lysozyme Variant HEL2X-Flex (Mutations R21Q, R73E, C76S, and C94S) within 5.0Å range:
probe atom residue distance (Å) B Occ
L:Cl302

b:55.3
occ:1.00
 OD1 L:ASP165 3.2 57.2 1.0
CE2 L:TYR173 3.6 40.2 1.0
NZ L:LYS103 3.7 49.2 1.0
N L:GLN166 3.7 39.2 1.0
CA L:ASP165 3.7 43.6 1.0
CE L:LYS103 3.9 48.4 1.0
CG L:ASP165 4.1 49.7 1.0
CB L:ASP165 4.2 46.2 1.0
C L:ASP165 4.2 38.9 1.0
CD2 L:TYR173 4.4 39.8 1.0
OH L:TYR173 4.4 42.5 1.0
OE2 L:GLU105 4.4 63.9 1.0
O L:THR164 4.5 38.9 1.0
CZ L:TYR173 4.5 43.0 1.0
CB L:GLN166 4.7 40.7 1.0
CA L:GLN166 4.8 40.1 1.0
N L:ASP165 4.8 41.5 1.0

Chlorine binding site 3 out of 9 in 6p4b

Go back to Chlorine Binding Sites List in 6p4b
Chlorine binding site 3 out of 9 in the HYHEL10 Fab Variant HYHEL10-4X (Heavy Chain Mutations L4F, Y33H, S56N, and Y58F) Bound to Hen Egg Lysozyme Variant HEL2X-Flex (Mutations R21Q, R73E, C76S, and C94S)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of HYHEL10 Fab Variant HYHEL10-4X (Heavy Chain Mutations L4F, Y33H, S56N, and Y58F) Bound to Hen Egg Lysozyme Variant HEL2X-Flex (Mutations R21Q, R73E, C76S, and C94S) within 5.0Å range:
probe atom residue distance (Å) B Occ
L:Cl303

b:56.7
occ:1.00
 NE2 L:GLN124 3.3 38.5 1.0
CA L:GLY129 3.8 47.5 1.0
CD H:LYS143 3.8 41.1 1.0
CG L:GLN124 3.9 34.5 1.0
CD L:GLN124 4.1 36.3 1.0
N L:GLY129 4.3 52.0 1.0
CE H:LYS143 4.3 46.8 1.0
OH H:TYR122 4.4 40.1 1.0
C L:GLY129 4.5 45.6 1.0
NZ H:LYS143 4.5 46.5 1.0
CG H:LYS143 4.7 41.1 1.0
CE2 H:TYR122 4.8 35.1 1.0
N L:ALA130 4.9 39.9 1.0

Chlorine binding site 4 out of 9 in 6p4b

Go back to Chlorine Binding Sites List in 6p4b
Chlorine binding site 4 out of 9 in the HYHEL10 Fab Variant HYHEL10-4X (Heavy Chain Mutations L4F, Y33H, S56N, and Y58F) Bound to Hen Egg Lysozyme Variant HEL2X-Flex (Mutations R21Q, R73E, C76S, and C94S)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of HYHEL10 Fab Variant HYHEL10-4X (Heavy Chain Mutations L4F, Y33H, S56N, and Y58F) Bound to Hen Egg Lysozyme Variant HEL2X-Flex (Mutations R21Q, R73E, C76S, and C94S) within 5.0Å range:
probe atom residue distance (Å) B Occ
L:Cl304

b:65.4
occ:1.00
 OG1 L:THR180 3.6 48.4 1.0
CD1 L:LEU160 3.7 36.8 1.0
CG2 L:THR178 3.7 36.0 1.0
CG2 H:THR176 3.8 29.6 1.0
CB H:THR176 4.0 30.4 1.0
CB L:THR178 4.1 34.1 1.0
CG2 H:VAL169 4.2 31.5 0.5
CG1 H:VAL169 4.3 30.3 0.5
OG1 H:THR176 4.3 30.9 1.0
CB L:LEU160 4.5 34.8 1.0
CG L:LEU160 4.6 35.8 1.0
OG1 L:THR178 4.9 32.2 1.0
CE H:LYS143 5.0 46.8 1.0
CB L:THR180 5.0 42.2 1.0

Chlorine binding site 5 out of 9 in 6p4b

Go back to Chlorine Binding Sites List in 6p4b
Chlorine binding site 5 out of 9 in the HYHEL10 Fab Variant HYHEL10-4X (Heavy Chain Mutations L4F, Y33H, S56N, and Y58F) Bound to Hen Egg Lysozyme Variant HEL2X-Flex (Mutations R21Q, R73E, C76S, and C94S)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 5 of HYHEL10 Fab Variant HYHEL10-4X (Heavy Chain Mutations L4F, Y33H, S56N, and Y58F) Bound to Hen Egg Lysozyme Variant HEL2X-Flex (Mutations R21Q, R73E, C76S, and C94S) within 5.0Å range:
probe atom residue distance (Å) B Occ
L:Cl305

b:36.4
occ:1.00
 O L:HOH456 3.1 38.3 1.0
O L:HOH448 3.3 38.3 1.0
N L:ASP1 3.4 30.3 1.0
CA L:ASP1 4.9 30.0 1.0

Chlorine binding site 6 out of 9 in 6p4b

Go back to Chlorine Binding Sites List in 6p4b
Chlorine binding site 6 out of 9 in the HYHEL10 Fab Variant HYHEL10-4X (Heavy Chain Mutations L4F, Y33H, S56N, and Y58F) Bound to Hen Egg Lysozyme Variant HEL2X-Flex (Mutations R21Q, R73E, C76S, and C94S)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 6 of HYHEL10 Fab Variant HYHEL10-4X (Heavy Chain Mutations L4F, Y33H, S56N, and Y58F) Bound to Hen Egg Lysozyme Variant HEL2X-Flex (Mutations R21Q, R73E, C76S, and C94S) within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cl201

b:52.3
occ:1.00
 CE2 C:PHE38 3.7 19.7 1.0
O C:HOH384 3.9 44.1 1.0
CZ3 C:TRP123 3.9 25.7 1.0
CD C:ARG5 4.1 30.7 1.0
CB C:ARG5 4.2 28.9 1.0
CZ C:PHE38 4.2 19.6 1.0
CD C:LYS33 4.2 24.7 1.0
NZ C:LYS33 4.2 31.0 1.0
CE C:LYS33 4.4 28.6 1.0
CE3 C:TRP123 4.6 27.8 1.0
CG C:ARG5 4.7 28.4 1.0
NE C:ARG5 4.7 31.1 1.0
CD2 C:PHE38 4.8 20.2 1.0
CH2 C:TRP123 4.9 30.1 1.0
CG C:LYS33 4.9 25.2 1.0

Chlorine binding site 7 out of 9 in 6p4b

Go back to Chlorine Binding Sites List in 6p4b
Chlorine binding site 7 out of 9 in the HYHEL10 Fab Variant HYHEL10-4X (Heavy Chain Mutations L4F, Y33H, S56N, and Y58F) Bound to Hen Egg Lysozyme Variant HEL2X-Flex (Mutations R21Q, R73E, C76S, and C94S)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 7 of HYHEL10 Fab Variant HYHEL10-4X (Heavy Chain Mutations L4F, Y33H, S56N, and Y58F) Bound to Hen Egg Lysozyme Variant HEL2X-Flex (Mutations R21Q, R73E, C76S, and C94S) within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cl202

b:49.0
occ:1.00
 O C:HOH306 3.2 37.7 1.0
CG1 C:ILE88 3.5 26.4 1.0
N C:ILE88 3.6 28.2 1.0
CE1 C:HIS15 3.7 29.8 1.0
CA C:ASP87 4.1 35.6 1.0
NH2 C:ARG14 4.1 44.9 1.0
CZ C:PHE3 4.1 23.9 1.0
O C:SER86 4.2 30.4 1.0
OD1 C:ASP87 4.2 39.3 1.0
CB C:ALA11 4.3 21.7 1.0
CD1 C:ILE88 4.3 26.3 1.0
CB C:ILE88 4.3 26.3 1.0
C C:ASP87 4.3 28.4 1.0
CG2 C:ILE88 4.4 26.4 1.0
NE2 C:HIS15 4.4 31.6 1.0
ND1 C:HIS15 4.5 33.5 1.0
CA C:ILE88 4.5 26.5 1.0
CE2 C:PHE3 4.7 24.9 1.0
CG C:ASP87 4.9 42.9 1.0
CB C:ASP87 4.9 36.3 1.0
C C:SER86 5.0 31.6 1.0
N C:ASP87 5.0 29.5 1.0

Chlorine binding site 8 out of 9 in 6p4b

Go back to Chlorine Binding Sites List in 6p4b
Chlorine binding site 8 out of 9 in the HYHEL10 Fab Variant HYHEL10-4X (Heavy Chain Mutations L4F, Y33H, S56N, and Y58F) Bound to Hen Egg Lysozyme Variant HEL2X-Flex (Mutations R21Q, R73E, C76S, and C94S)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 8 of HYHEL10 Fab Variant HYHEL10-4X (Heavy Chain Mutations L4F, Y33H, S56N, and Y58F) Bound to Hen Egg Lysozyme Variant HEL2X-Flex (Mutations R21Q, R73E, C76S, and C94S) within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cl301

b:58.2
occ:1.00
 OG1 B:THR116 3.3 43.7 1.0
N B:THR116 3.4 39.7 1.0
CB B:THR116 3.7 41.8 1.0
CD2 B:LEU11 4.1 31.2 1.0
CA B:LYS115 4.1 38.2 1.0
CA B:THR116 4.2 41.4 1.0
C B:LYS115 4.2 39.5 1.0
O B:ALA114 4.5 35.1 1.0
O B:HOH407 4.5 27.6 1.0
CB B:LYS115 4.9 40.9 1.0
NZ B:LYS13 4.9 41.8 1.0

Chlorine binding site 9 out of 9 in 6p4b

Go back to Chlorine Binding Sites List in 6p4b
Chlorine binding site 9 out of 9 in the HYHEL10 Fab Variant HYHEL10-4X (Heavy Chain Mutations L4F, Y33H, S56N, and Y58F) Bound to Hen Egg Lysozyme Variant HEL2X-Flex (Mutations R21Q, R73E, C76S, and C94S)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 9 of HYHEL10 Fab Variant HYHEL10-4X (Heavy Chain Mutations L4F, Y33H, S56N, and Y58F) Bound to Hen Egg Lysozyme Variant HEL2X-Flex (Mutations R21Q, R73E, C76S, and C94S) within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Cl201

b:42.6
occ:1.00
 N D:ILE88 3.3 23.1 1.0
CG1 D:ILE88 3.7 22.4 1.0
CA D:ASP87 3.7 28.2 1.0
CE1 D:HIS15 3.8 26.6 1.0
NH1 D:ARG14 3.8 26.0 0.5
C D:ASP87 4.0 24.4 1.0
O D:SER86 4.1 28.0 1.0
OD1 D:ASP87 4.1 38.0 1.0
CB D:ILE88 4.2 21.8 1.0
CG2 D:ILE88 4.2 23.1 1.0
CZ D:PHE3 4.3 23.2 1.0
CA D:ILE88 4.3 21.0 1.0
CG D:ASP87 4.4 35.1 1.0
CD1 D:ILE88 4.4 23.1 1.0
CB D:ALA11 4.5 17.2 1.0
ND1 D:HIS15 4.5 34.3 1.0
NE2 D:HIS15 4.6 31.5 1.0
CB D:ASP87 4.6 31.6 1.0
N D:ASP87 4.7 28.0 1.0
C D:SER86 4.8 26.7 1.0
CZ D:ARG14 4.9 27.6 0.5
CE2 D:PHE3 4.9 22.6 1.0

Reference:

D.B.Langley, D.Christ. Resolution of Self-Reactivity Upon Vaccination with A Conformationally Flexible Antigen. To Be Published.
Page generated: Sat Dec 12 13:28:48 2020

Last articles

Zn in 8WB0
Zn in 8WAX
Zn in 8WAU
Zn in 8WAZ
Zn in 8WAY
Zn in 8WAV
Zn in 8WAW
Zn in 8WAT
Zn in 8W7M
Zn in 8WD3
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy