Chlorine in PDB 6pfr: RSEGFP2 with A Chlorinated Chromophore in the Fluorescent on-State

The structure of RSEGFP2 with A Chlorinated Chromophore in the Fluorescent on-State, PDB code: 6pfr was solved by J.Chang, M.G.Romei, S.G.Boxer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.37 / 1.51
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	50.699, 62.489, 67.591, 90.00, 90.00, 90.00
R / Rfree (%)	18.8 / 21.2

The binding sites of Chlorine atom in the RSEGFP2 with A Chlorinated Chromophore in the Fluorescent on-State (pdb code 6pfr). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the RSEGFP2 with A Chlorinated Chromophore in the Fluorescent on-State, PDB code: 6pfr:

Chlorine binding site 1 out of 1 in the RSEGFP2 with A Chlorinated Chromophore in the Fluorescent on-State


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of RSEGFP2 with A Chlorinated Chromophore in the Fluorescent on-State within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl68 b:34.2 occ:1.00 CL A:OHD68 0.0 34.2 1.0 CE2 A:OHD68 1.8 22.3 1.0 CD2 A:OHD68 2.7 26.8 1.0 CZ A:OHD68 2.7 27.4 1.0 OH A:OHD68 3.0 30.1 1.0 CG1 A:VAL151 3.1 46.6 1.0 CB A:HIS149 3.5 25.0 1.0 ND1 A:HIS149 3.6 25.6 1.0 OG1 A:THR204 3.7 26.7 1.0 CD1 A:ILE168 3.7 33.5 1.0 CE2 A:PHE166 3.8 20.4 1.0 CG A:HIS149 3.9 25.6 1.0 CG2 A:OHD68 4.0 22.7 1.0 CE1 A:OHD68 4.0 27.9 1.0 CG2 A:THR204 4.1 34.0 1.0 CD2 A:PHE166 4.1 21.6 1.0 CB A:THR204 4.4 27.4 1.0 CZ A:PHE166 4.5 23.2 1.0 CB A:VAL151 4.5 42.0 1.0 CD1 A:OHD68 4.5 23.6 1.0 CG2 A:THR63 4.7 20.8 1.0 CE1 A:HIS149 4.7 25.4 1.0 CG2 A:VAL151 4.8 36.0 1.0 CA A:HIS149 4.8 26.2 1.0 CG A:PHE166 4.9 25.4 1.0 CG1 A:ILE168 5.0 39.7 1.0

J.Chang, M.G.Romei, S.G.Boxer. Structural Evidence of Photoisomerization Pathways in Fluorescent Proteins. J.Am.Chem.Soc. V. 141 15504 2019.
ISSN: ESSN 1520-5126
PubMed: 31533429
DOI: 10.1021/JACS.9B08356