Chlorine in PDB 6pft: RSEGFP2 with A Chlorinated Chromophore in the Non-Fluorescent Off- State

The structure of RSEGFP2 with A Chlorinated Chromophore in the Non-Fluorescent Off- State, PDB code: 6pft was solved by J.Chang, M.G.Romei, S.G.Boxer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	34.31 / 1.45
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	51.014, 62.716, 68.619, 90.00, 90.00, 90.00
R / Rfree (%)	15 / 17.6

The binding sites of Chlorine atom in the RSEGFP2 with A Chlorinated Chromophore in the Non-Fluorescent Off- State (pdb code 6pft). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the RSEGFP2 with A Chlorinated Chromophore in the Non-Fluorescent Off- State, PDB code: 6pft:

Chlorine binding site 1 out of 1 in the RSEGFP2 with A Chlorinated Chromophore in the Non-Fluorescent Off- State


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of RSEGFP2 with A Chlorinated Chromophore in the Non-Fluorescent Off- State within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl68 b:26.7 occ:1.00 CL A:OHD68 0.0 26.7 1.0 CE2 A:OHD68 1.7 19.5 1.0 CZ A:OHD68 2.6 24.2 1.0 CD2 A:OHD68 2.7 19.0 1.0 OH A:OHD68 2.9 26.4 1.0 CB A:LEU202 3.5 16.1 1.0 CB A:THR204 3.7 16.0 1.0 O A:LEU202 3.7 20.4 1.0 CG2 A:THR204 3.8 19.8 1.0 CE1 A:OHD68 3.9 24.2 1.0 C A:LEU202 4.0 15.1 1.0 CG2 A:VAL151 4.0 20.5 1.0 CG2 A:OHD68 4.0 19.3 1.0 CG1 A:VAL225 4.0 16.2 1.0 CB A:VAL151 4.2 20.2 1.0 N A:THR204 4.4 15.3 1.0 CD1 A:LEU202 4.4 17.7 1.0 CD1 A:OHD68 4.4 22.7 1.0 CA A:LEU202 4.4 18.8 1.0 C A:SER203 4.5 17.3 1.0 N A:SER203 4.6 17.5 1.0 OG1 A:THR204 4.6 20.4 1.0 CG A:LEU202 4.6 16.7 1.0 CA A:THR204 4.6 13.7 1.0 CE1 A:HIS149 4.7 21.5 1.0 CA A:SER203 4.8 18.6 0.4 O A:SER203 4.8 19.0 1.0 CA A:SER203 4.9 18.1 0.6 CG2 A:VAL225 4.9 16.8 1.0 O A:HIS149 5.0 22.4 1.0 CD2 A:LEU70 5.0 16.4 1.0 N A:VAL151 5.0 17.4 1.0

J.Chang, M.G.Romei, S.G.Boxer. Structural Evidence of Photoisomerization Pathways in Fluorescent Proteins. J.Am.Chem.Soc. V. 141 15504 2019.
ISSN: ESSN 1520-5126
PubMed: 31533429
DOI: 10.1021/JACS.9B08356