Chlorine in PDB 6rl0: Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I

Enzymatic activity of Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I

All present enzymatic activity of Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I:
1.7.2.4;

Protein crystallography data

The structure of Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I, PDB code: 6rl0 was solved by L.Zhang, A.Wuest, B.Prasser, C.Mueller, O.Einsle, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 48.91 / 1.78
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 108.916, 73.372, 136.342, 90.00, 95.07, 90.00
R / Rfree (%) 15.7 / 19.2

Other elements in 6rl0:

The structure of Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I also contains other interesting chemical elements:

Potassium (K) 4 atoms
Calcium (Ca) 4 atoms
Copper (Cu) 24 atoms
Sodium (Na) 4 atoms

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I (pdb code 6rl0). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I, PDB code: 6rl0:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in 6rl0

Go back to Chlorine Binding Sites List in 6rl0
Chlorine binding site 1 out of 4 in the Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl702

b:22.8
occ:0.68
 CE1 A:HIS382 2.8 20.2 0.5
ND1 A:HIS382 3.0 22.1 0.5
O A:HOH1075 3.1 34.6 1.0
NH1 A:ARG181 3.1 30.5 1.0
ND1 A:HIS382 3.3 21.1 0.5
N A:CYS328 3.3 21.9 1.0
ND2 A:ASN329 3.4 23.1 1.0
CE1 A:HIS382 3.4 21.2 0.5
NH2 A:ARG181 3.5 29.1 1.0
CB A:ASN241 3.6 21.1 1.0
CA A:GLY327 3.7 23.0 1.0
O A:CYS328 3.7 23.8 1.0
CZ A:ARG181 3.7 30.2 1.0
CG A:ASN241 3.7 25.7 1.0
CG A:ASN329 3.9 23.0 1.0
OD1 A:ASN329 3.9 21.0 1.0
C A:GLY327 4.0 21.7 1.0
C A:CYS328 4.1 23.6 1.0
NE2 A:HIS382 4.1 18.9 0.5
OD1 A:ASN241 4.2 31.5 1.0
CE1 A:HIS433 4.2 33.0 1.0
ND2 A:ASN241 4.2 27.4 1.0
CA A:CYS328 4.3 22.4 1.0
CG A:HIS382 4.3 20.1 0.5
O A:HOH1122 4.3 20.3 1.0
CG A:HIS382 4.6 20.6 0.5
NE2 A:HIS382 4.7 21.7 0.5
ND1 A:HIS433 4.7 29.4 1.0
CB A:CYS328 4.8 24.3 1.0
CD2 A:HIS382 4.9 21.1 0.5
N A:ASN329 4.9 20.4 1.0
N A:GLY327 4.9 21.5 1.0
CA A:ASN241 5.0 19.4 1.0

Chlorine binding site 2 out of 4 in 6rl0

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Chlorine binding site 2 out of 4 in the Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cl702

b:28.1
occ:0.84
 CD2 B:HIS382 3.0 25.2 0.5
NE2 B:HIS382 3.0 25.2 0.5
NH1 B:ARG181 3.1 33.1 1.0
O B:HOH1026 3.2 39.2 1.0
ND1 B:HIS382 3.3 21.1 0.5
N B:CYS328 3.3 20.3 1.0
ND2 B:ASN329 3.3 24.9 1.0
CE1 B:HIS382 3.4 21.9 0.5
NH2 B:ARG181 3.5 34.1 1.0
CA B:GLY327 3.6 22.6 1.0
CB B:ASN241 3.6 25.6 1.0
O B:CYS328 3.7 22.0 1.0
CZ B:ARG181 3.8 32.7 1.0
CG B:ASN241 3.8 26.4 1.0
CG B:ASN329 3.9 25.7 1.0
OD1 B:ASN329 3.9 24.7 1.0
C B:GLY327 4.0 21.1 1.0
C B:CYS328 4.0 24.9 1.0
OD1 B:ASN241 4.2 38.2 1.0
CE1 B:HIS433 4.2 36.6 1.0
CA B:CYS328 4.3 22.7 1.0
ND2 B:ASN241 4.3 28.1 1.0
CE1 B:HIS382 4.3 24.7 0.5
CG B:HIS382 4.3 24.8 0.5
O B:HOH1066 4.5 23.8 1.0
CG B:HIS382 4.6 20.6 0.5
NE2 B:HIS382 4.7 22.8 0.5
ND1 B:HIS433 4.7 34.7 1.0
CB B:CYS328 4.8 24.1 1.0
N B:ASN329 4.8 21.3 1.0
N B:GLY327 4.9 21.1 1.0
ND1 B:HIS382 5.0 24.7 0.5

Chlorine binding site 3 out of 4 in 6rl0

Go back to Chlorine Binding Sites List in 6rl0
Chlorine binding site 3 out of 4 in the Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cl702

b:26.3
occ:0.72
 CE1 C:HIS382 2.9 20.0 0.5
ND1 C:HIS382 3.1 20.9 0.5
O C:HOH1040 3.1 42.5 1.0
NH1 C:ARG181 3.2 30.0 1.0
N C:CYS328 3.2 22.8 1.0
ND1 C:HIS382 3.2 16.1 0.5
ND2 C:ASN329 3.4 22.6 1.0
CE1 C:HIS382 3.5 17.1 0.5
NH2 C:ARG181 3.5 34.5 1.0
CB C:ASN241 3.6 22.4 1.0
O C:CYS328 3.6 23.9 1.0
CA C:GLY327 3.6 21.5 1.0
CG C:ASN241 3.7 28.8 1.0
CZ C:ARG181 3.8 32.7 1.0
OD1 C:ASN329 3.8 22.6 1.0
CG C:ASN329 3.9 23.5 1.0
C C:GLY327 3.9 23.3 1.0
C C:CYS328 4.0 22.1 1.0
OD1 C:ASN241 4.1 33.9 1.0
CE1 C:HIS433 4.2 39.6 1.0
NE2 C:HIS382 4.2 20.3 0.5
CA C:CYS328 4.2 22.4 1.0
ND2 C:ASN241 4.2 31.7 1.0
O C:HOH1075 4.4 23.4 1.0
CG C:HIS382 4.4 20.3 0.5
CG C:HIS382 4.5 17.3 0.5
ND1 C:HIS433 4.7 34.6 1.0
CB C:CYS328 4.8 21.4 1.0
NE2 C:HIS382 4.8 17.2 0.5
N C:ASN329 4.8 20.2 1.0
N C:GLY327 4.9 20.2 1.0
CD2 C:HIS382 5.0 20.3 0.5
CA C:ASN241 5.0 20.1 1.0

Chlorine binding site 4 out of 4 in 6rl0

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Chlorine binding site 4 out of 4 in the Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Cl702

b:26.7
occ:0.71
 O D:HOH1039 3.1 36.5 1.0
CD2 D:HIS382 3.1 21.4 0.5
NE2 D:HIS382 3.1 21.2 0.5
NH1 D:ARG181 3.2 35.6 1.0
N D:CYS328 3.3 23.8 1.0
CD2 D:HIS382 3.4 19.6 0.6
ND2 D:ASN329 3.4 25.1 1.0
NH2 D:ARG181 3.5 39.4 1.0
CB D:ASN241 3.5 25.3 1.0
CA D:GLY327 3.6 22.2 1.0
O D:CYS328 3.7 26.3 1.0
CG D:ASN241 3.7 29.0 1.0
CZ D:ARG181 3.8 37.8 1.0
NE2 D:HIS382 3.9 23.8 0.6
OD1 D:ASN241 3.9 36.7 1.0
CG D:ASN329 4.0 23.6 1.0
OD1 D:ASN329 4.0 22.2 1.0
C D:GLY327 4.0 23.2 1.0
C D:CYS328 4.1 24.7 1.0
CE1 D:HIS433 4.2 32.6 1.0
CA D:CYS328 4.2 24.3 1.0
ND2 D:ASN241 4.3 27.0 1.0
CG D:HIS382 4.4 22.2 0.5
O D:HOH1027 4.4 24.4 1.0
CE1 D:HIS382 4.4 22.2 0.5
CG D:HIS382 4.6 21.4 0.6
ND1 D:HIS433 4.7 31.3 1.0
CB D:CYS328 4.7 25.9 1.0
N D:ASN329 4.9 22.9 1.0
N D:GLY327 4.9 21.6 1.0
CA D:ASN241 4.9 22.2 1.0
O D:HIS326 5.0 22.9 1.0

Reference:

L.Zhang, A.Wust, B.Prasser, C.Muller, O.Einsle. Functional Assembly of Nitrous Oxide Reductase Provides Insights Into Copper Site Maturation. Proc.Natl.Acad.Sci.Usa V. 116 12822 2019.
ISSN: ESSN 1091-6490
PubMed: 31189605
DOI: 10.1073/PNAS.1903819116
Page generated: Sat Dec 12 13:36:51 2020

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